Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,484 C→A 18.9% Q8K (CAA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4840CA18.9% 162.7 / 7.4 76Q8K (CAA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (15/45);  new base A (9/6);  total (25/51)
Fisher's exact test for biased strand distribution p-value = 1.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206249‑3206709
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                    <  2:724441/239‑1 (MQ=255)
     cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACTGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                               <  1:655680/239‑1 (MQ=255)
      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                 <  1:415957/236‑1 (MQ=255)
      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                 <  1:611726/236‑1 (MQ=255)
      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                 <  1:1126239/236‑1 (MQ=255)
       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGACCAATAAAAAAACTGGAGTTGCCATTATGTGGGGAAGTAAATATCAAAAGGCAgg                                                                                                                                                                                                                             >  1:348989/1‑238 (MQ=255)
          ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTAGACAAATACAAAAACAGGAGTTTTCATAGTGAGCGAAAGTAAAAAACAAAAAGCaaggaa                                                                                                                                                                                                                          >  2:1096815/1‑234 (MQ=255)
            aaTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                                                                                                                                                       <  1:1131454/239‑1 (MQ=255)
            aaTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                                                                                                                                                       <  2:1150511/239‑1 (MQ=255)
              ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                                                                                                                                                     <  1:1121145/239‑1 (MQ=255)
                  ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCCAACAAATACAAAAAATAGAGATGCCCTGATGGGGGAAAGTAATTAGCAAAAAGGAAGGAACAAGTTgt                                                                                                                                                                                                                >  1:329533/1‑238 (MQ=255)
                   cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACAGGGGGTGGCATGTTGAGCGAAAGAAATAAAAAAAAGACAGGGAATaaagttag                                                                                                                                                                                                                >  1:781108/1‑233 (MQ=255)
                   cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                 <  1:918535/223‑1 (MQ=255)
                         tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                         <  1:149676/238‑1 (MQ=255)
                         tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                        <  2:857712/239‑1 (MQ=255)
                             aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTATACAAATACAAAAAAAGGGGTTGGCCTGATGAGGGGAAGTAATAAGAAAAAGACAGAGAATAAGATGACAGAGATTTTc                                                                                                                                                                                                    >  1:529893/1‑239 (MQ=255)
                                  cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAACAAAAAATGAGGTTGCCATGATGAGCGGAAGTAATAAACAAAAAGCAGTGAAAAAGTTGACAGAGATTGTGGCTa                                                                                                                                                                                                >  1:469486/1‑238 (MQ=255)
                                          aTTTCGCAAAACATTGCCATCCCCGTCTCTGGTGAAATATTCATTCTTATTTTCGCGTTGATTCAGGATCTCTTAATTACATATTCACGGTGACAAAAAATATAAAACCACATTTTTAGTTGGAGTTTGTGGCGGTGAATTTTCCCAGAAAAATACAAAACGTGGGGTCCCCTTGATGAGCAAAAGTATTAAGCAACAGGCAGTGAATAAGTCGCCAGATATGGTGCCTATCttgtcc                                                                                                                                                                                        <  2:329533/238‑5 (MQ=255)
                                            ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGAAAAAAACAAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAACAAAAAGCAGCGAATAAAGTTGCAGAGATTGTCGCTAAATTTatcac                                                                                                                                                                                      >  2:711860/1‑234 (MQ=255)
                                                 acagcATTGCCATTCCCTGCTCTGGCGCAATATTCATTTTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                                                                                                                                                               <  1:1143804/235‑1 (MQ=255)
                                                 aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTATTTTTTCACACAAATAAAAAAAATCGGGTTGCCATTATGAGGGAGAGTAATAAACAAACCGCAGTGAATAAGGTGACAGGGATTGTCGTCAACCTTCACGCCCTGAt                                                                                                                                                                               >  1:968500/1‑239 (MQ=255)
                                                           cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                     <  1:192075/239‑1 (MQ=255)
                                                           cATTCCCTGCTCGGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                                                                                                                                                                      <  1:966616/238‑1 (MQ=255)
                                                             ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGATTTTTTTGCTCCATATACACAAAAACCAGGTGTCATGTTTTGGCAAAGTAAAAAAACACAGACACAGAATAAGTTTACAGCGATTGTCGTCAACTTTTCCGCCCTCATTTTTACCCTAAt                                                                                                                                                                   >  2:26330/1‑239 (MQ=255)
                                                               cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACACAAAAACAAAAACACGAAGTGTCATGATGGGCGAAAAGAAAAAACAACAAGCAAGGAATAAAATGACAAAGAATTTCGCTAACTTTACAGCCATGATTTTTAACCGAATg                                                                                                                                                                  >  1:618797/1‑238 (MQ=255)
                                                                ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATACAAAAACTAGAGGTGGCATGATGAGGGAAAGAAATAAAAAACAAACAGGGAATAAAATGACAAAGAATTTTGCCAAATTTTTCCCCCTAATTTTTACCCCaacttt                                                                                                                                                                >  2:1143804/1‑235 (MQ=255)
                                                                     tcgggacaattatTAATTGTTATTTCCCGTTTCATCCACAAAGTTTTAATTACATTTTCATGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTGGTTTGGGGCGTTAAATTTTTCCAGCAAAATAAAAAAACGGGAGTTGCAATGATGGGCGAAAGTAATAAGCAACAGGCAGTAAATAAGGTGACAGGGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                                                                                                                                                            <  2:920714/228‑1 (MQ=255)
                                                                         gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCGCCAAAAAAAAAAAATGGAGTTGTCATGATGAGCGGAAGGAATAAAGAACAAGCAATGAATAAGTTGACAAAGAGTTTCGCTAACTTTACCGCCCTGATTTCTAACCGCATGCCtgctga                                                                                                                                                          >  1:325980/1‑236 (MQ=255)
                                                                         gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGGAAAAAAAAAAAAATTGAGGTGGCCAGATGAGAGGAAGTAAAAAAAAAAAAGAAAGGGAAAAATTGAAAGAGAATTGTGCTTAACTTTACGCCCATATTTTTACCCCAAAGCCctatgaagg                                                                                                                                                       >  1:561769/1‑236 (MQ=255)
                                                                               tatttattCTTATTTTGGCTTTGATTCACGAACTGTTAATTAAATATTCGGGGTGGAAAAAAATAAAAAACCACATTTTAAGTGGTATTTGGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGAGGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATGGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                 <  2:267027/239‑1 (MQ=255)
                                                                               tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                 <  1:1096815/239‑1 (MQ=255)
                                                                               tatGCATGCTTTTTTGCGCGTTGATTCACGAACTATTAATTAAATATTCACGGTGGAAAAAAATAAAAAACCACATTTTGAGTGGTAGTTTGGGGGGGTAAATTTTTCAAAAAAAAAAAAAAACCGGGAGTTGCCATGAGGAGGGAAAGAAATAAGAAACAGGCAGTAAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                 <  2:1161809/239‑1 (MQ=255)
                                                                                       cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                         <  2:1053611/239‑1 (MQ=255)
                                                                                         tATTTTCGTTTTGATTCACGAACTGTTAATTAAATATTCACGGTGACAAAAAATATAAAACCACATTTTTGGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAAAAAAACGGGGGTGGCCATGATGGGCGAAAGAAATAAGAAACAGGAAGTAAATAAGTGGAAAGGGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  2:164482/239‑1 (MQ=255)
                                                                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  1:990616/239‑1 (MQ=255)
                                                                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGCGGAAGGAAAAAACAACAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGCCATAATTTCTACCCGAATTCCTGATGACGGGGGGGAtaaaataaaa                                                                                                                                       >  1:516344/1‑239 (MQ=255)
                                                                                         tATTTACGCGTTGATTCACGATCTTCTAATCACATATGCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  1:903891/239‑1 (MQ=255)
                                                                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCATAACAAAAAAAAAAATAGGGGTGCCCTGATGAAGAAAAGTAAAAAAAAAAAAGCACGGAATAAAATTACAAAAAAGGTTGCCTACTTTAACCCCACAATTTTTACCCCAAATGCTGTTGAAGGGGTGGGTAAAACaaaaa                                                                                                                                      >  2:731474/1‑238 (MQ=255)
                                                                                                 cttttaTTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTAACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa                                                                                                                               <  2:529893/234‑1 (MQ=255)
                                                                                                           acgaattttAAATACAATATTCAGGGTGGAAAAAAATAAAAAACAACATTTTTAGGGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACGGGCATTGAATAAGTGGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGa                                                                                                                      <  2:618797/232‑1 (MQ=255)
                                                                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAGAAATAAACAAAAAGCCGGGAAAAAAATGACAAAGAATGTTGCTAACTTTACCTCCAATGATTTTTCCCCGAAGGCTGATTATGCGGGGGTGAAAATAAAAAACCTAAAAGGTGCAGCAAcgg                                                                                                                 >  2:96564/1‑237 (MQ=255)
                                                                                                                      ttACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTATTGATAGTTGGTGGCGGTGAATTTGTCCAGACAAAAAAAAAAACTGGATTTGCAATGAGAAGCGAAATTAATAAGCAACAGGCAGGAAATAATTTGACAGAGATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGTATGCCGAAACGTCGTCGAt                                                                                                          <  1:165772/239‑1 (MQ=255)
                                                                                                                            ttttCAGGGTGGAAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGAAGTTGCAAAGATGAGCAAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:844547/237‑1 (MQ=255)
                                                                                                                            tatTCGGGGTGGAAAAAAATATAAAACCACATTGTTAGTGGTAGTTTGTGGGGGTAAATTTTTCCAGAAAAAAAAAAAAACTGGATTTGCCAGAATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTAACAGAGATGGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:52520/239‑1 (MQ=255)
                                                                                                                            tatTCACGGTGGAAAAAAAAAAAAACCCACATTTTTAGTGGTATTTGGGGGCGGGAATTTTTCCCAAAAAAATAAAAAAGCGGGAGTTGCCATGAGGAGTAAAAAAAAAAAACAACAGGAATTGAATAATTTGACAGGGATTGTCCCTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:503169/239‑1 (MQ=255)
                                                                                                                                   ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCATAAAAATATAAAAAAATGAGTTGACATGATGAGCGAAAGGAATAAGAAACAAGCAGTGAAAAAGATGAGAAAGAATTTCTCTAAATTTTACCCCCTGATTTTTATCCGAATCCCCTGTGACGTGGTGGATAAACAAAAACAGCTAAAGAATACCGAAAATACGTCGATGGGGAAAAatat                                                                                              >  1:539887/1‑239 (MQ=255)
                                                                                                                                    gTGGCAAAAAATATAAAACCACATTTTTAGTGGAAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:96564/238‑1 (MQ=255)
                                                                                                                                    gTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAAACAAAAACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:538784/238‑1 (MQ=255)
                                                                                                                                    gTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTGGAGGCGGTAAATTTTTCCAGACAAAAACAAAAACGGGAGTGGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGTTGCAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  2:1131230/238‑1 (MQ=255)
                                                                                                                                     tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGGTGCCATGATGAGCGGAAAGAATAAAAAAAAGGCAGAGAATAAGATGACAGAGATTGTCGCTAACTTTTCCGCCATGATTTCTACCCCCATGCCTGATGACGTGGTGGATAAACTAAAACAACTAAAAGATGCCGAAAAGTCGTCGCTGGGGGAAATTAttt                                                                                            >  1:52520/1‑237 (MQ=255)
                                                                                                                                     tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGTTTCCATGATGGGGGAAAGTAATAAAAAACAGGCAGTGGATAAAGTGACAGAGATTGTTGCTAAATTTTCCCCCCTGGTTTTTACCCCAAAGCCTGGTGACCGGGTGGATTAACTAAAACAACTAAATGATGCCGGAACGTCGTCGCTGGGGGAAAtttttt                                                                                            >  1:1177000/1‑235 (MQ=255)
                                                                                                                                     tGGAAAAAAATATAAAACCACATTTTAAGTGTGAGTTTGTGGCGGTGAATTTTTCCAAAAAAATAAAAAAACGGGAGTTGCCAGGAAAAGCAAAAGAAATAAGCAACAGTCATTAAATAAGTTAACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                             <  2:959124/238‑1 (MQ=255)
                                                                                                                                     gggaaaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTGCAAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                             <  1:1101985/234‑1 (MQ=255)
                                                                                                                                           aaaaTAAAAAACCACATTTTTAGTGGTAGTTGGGGGCGGGAAATTTTCCCAGAAAAATAAAAAAACTGGAGTTGCAAGGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                      <  1:416227/239‑1 (MQ=255)
                                                                                                                                             aaTATAAAACAAAATTTTTAGGGGTAGGTGGGGGGGGGGAATTTTACCAGAAAAAACAAAAACGGGAAGTGGCCAGGATGAGCAAAAGAAAAAAGCAACAGGCAGGAAATAAGTTGAAAGGAATGGTCGTAATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                    <  2:968500/239‑1 (MQ=255)
                                                                                                                                             aaTAAAAACCCACATTTTAAGTGGGATTTTGGGGGGGTAAATTTTACAAAAGAAATAAAAAAAAGGGAGTTGCCATAAAGAGCAAAAGTAATAAGAAACAGGCATGAAAAAATTAGACAAAGTTTGTCGCAAATTGTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                    <  1:1051917/239‑1 (MQ=255)
                                                                                                                                                        acaTTTTAAGGGGTAGTTTGTGGCGATAATTTTTCCCAATAAATAAGAAAAACGGAAGTTGCAGAGATGAACAAAAGAAATAAGCAACAGGCAGAGAATAGGTTGACAGAAATTGTCAAAAAGTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                         <  1:568561/239‑1 (MQ=255)
                                                                                                                                                            ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAAATGAGTTGCCCTTATGAGCGGAAGGAATAAACAAAAAGCAGAGAATAAATTGACAGAGAATTTCGCCTACTTTTACGCCCTGATTTCTACCCGAAAGCCTTATGACGTGGTGGGTAAAATAAAACAACTAAAAGATGCCGAAACGACGGCGATGGGGAAAAATATTTACCAAACGATGTTTGaccacct                                                                      >  1:219713/1‑236 (MQ=255)
                                                                                                                                                                      ggttgtgggCGGTGAATTTTTCCAAAAAAATACAAAAACGGGAGTGCCGATGATGAGCAAAAGTAATAAGCAGCAGGCGGTGAATAAGTTGACAGAGATGTTCGTTAATTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:26330/232‑1 (MQ=255)
                                                                                                                                                                      attttGTGGCGGTAAATTTTTCCAGAAAAATACAAAAACGGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACGGGAAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:220838/237‑1 (MQ=255)
                                                                                                                                                                      aGTTTGTGGCGGTAAATTTTTCAAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCATTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:856308/239‑1 (MQ=255)
                                                                                                                                                                       ttttGGGGCGGGAAATTTTTCCAGAAAAATACAAAAACGGGATTTGCCAGGATGGGCAAAAGTAAAAACCAACAGGAAGTGAAAAATTGGACAGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAt                                                          <  2:561769/238‑1 (MQ=255)
                                                                                                                                                                                   gAATTTTTCCAGACAAATACAAAAACTGAAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCg                                              <  1:731474/239‑1 (MQ=255)
                                                                                                                                                                                     aTTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAATAAAAAGAAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCg                                              <  2:348989/237‑1 (MQ=255)
                                                                                                                                                                                        tttCCAGACAAATACAAAAACTGGATTTGCCATGATGAGCAAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  1:318781/239‑1 (MQ=255)
                                                                                                                                                                                        tttCCAGAAAAATACAAAAACGGGAGTTGCCAGGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTAATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  1:711860/239‑1 (MQ=255)
                                                                                                                                                                                           acaaGAAAAAAAAAAAAACGGGAGTTGCCATGATGGGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                                       <  2:516344/236‑1 (MQ=255)
                                                                                                                                                                                                aaaaaTACAAAAACGGGAAGTCCCATGAGGAGAAAAAGAAATAAGCAACAGGCGGGAAATAAGTGGAAAGAGATTTTCGCAACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                  <  2:965156/237‑1 (MQ=255)
                                                                                                                                                                                                           aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                        >  2:1038302/1‑239 (MQ=255)
                                                                                                                                                                                                            aaCTGGAGTTCCGATGATGAGAGAAAGTAATAAGCAAAGGGCAGTAAATAAGTGGAAAGGGATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:219713/239‑1 (MQ=255)
                                                                                                                                                                                                            aaCGGGAGTTGCCTTGATGAGCGAAGGAAATAAGAAACAGGCAGTAAATAAGTTGACAGATATTGTCTCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:830309/239‑1 (MQ=255)
                                                                                                                                                                                                                 gAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGCCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGACACCGGGGAGAtt                  >  1:701767/1‑239 (MQ=255)
                                                                                                                                                                                                                       gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt              >  1:612925/1‑238 (MQ=255)
                                                                                                                                                                                                                         gatgatgAGCGAAAGTAATAAGCAACGGGCAGAGAATAAGTGGACAGAGTTTGCCGCTAACTTTACCGCCATTATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc            <  2:781108/237‑1 (MQ=255)
                                                                                                                                                                                                                                  cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  2:572617/1‑239 (MQ=255)
                                                                                                                                                                                                                                  cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  2:1131542/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206249‑3206709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: