Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,782,913 | A→C | 18.2% | V250G (GTG→GGG) | gpsA ← | glycerol‑3‑phosphate dehydrogenase (NAD+) |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,782,913 | 0 | A | C | 18.2% | 114.4 / 4.5 | 74 | V250G (GTG→GGG) | gpsA | glycerol‑3‑phosphate dehydrogenase (NAD+) |
Reads supporting (aligned to +/- strand): ref base A (27/32); new base C (4/11); total (31/43) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA > NC_000913/3782679‑3783150 | aGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGTGACTGGTTGT‑CGGTACAGGTAAGCACCA‑g < 1:329126/239‑1 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGGCTTGGTGT‑CGGGTAAGGTAAACACCA‑AATCGCCa > 1:857832/1‑239 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCCTCACTGGTTGT‑CGGGACAGCGAAGCACCA‑AAACGCCa > 2:917818/1‑239 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGGTGT‑CGGTGAAGGGAAGGACCC‑AAACGCCa > 1:574333/1‑239 (MQ=255) tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTTT‑CCGTACCGGGGAGCACCaacacgcccagc > 1:433040/1‑227 (MQ=255) cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCa < 1:1125230/239‑1 (MQ=255) tcgcggcctttttttCCGAATTATAATCCTAGTTAAATTCCCTCTGTTGTTGGCATTTCAACGCGAACGCGATGCGCTCGTTGGCGGACTCCTTCCGTATCGCGCTAGCTTCCCACCACCAGTCCAATCTTCTCCTGCCCCCTTTGTGCATCAAGGCCAGGACCAACCGTCAGCCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGACAAGGTAAGCACCA‑GACCGCCAAGCCCCGCCAt < 2:699977/230‑1 (MQ=255) ccgcgcgcgTTTTTTCCTCAATATAATACTGAAGAAATTTCCTCGTTTATTGGCATTTCACCGCCGAAGCGATGCGCAGGTCCGCGGACTTCTTTCTTATTGCGTTGGCCTCCCACCCCCTGACCAATCTTCTCCGGCACGTTTGTTCCATCCATGCCCTGACCGACCTTCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGTTACAGGTAACCCCCA‑GACCGCCAAGCCCCGCCAt < 2:121969/238‑1 (MQ=255) gTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGT‑CGGTACGGGGTAGCACCA‑GATCCCCAAGCCCCCCCCTGCCCCTaa > 1:460549/1‑239 (MQ=255) gTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCC‑TCCACCACCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGTTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATaa < 1:1029668/238‑1 (MQ=255) ccGCAATATAATACTTGTTAAATTCCCTGGGTTATTGGATTTCCAACGCCGAAGCGATGCGCAAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGCCAAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGGCCAAACCGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GACCGCAAAGCCCCGCCATGCCCATAA‑AGGT‑GGc < 2:940548/239‑1 (MQ=255) atatAATCCTTGTTAAATTTCCTCGTTTTTGGGCATTCCAACGCCGAAGCGATGCGCCGGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GACCGCAAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGt < 2:471277/239‑1 (MQ=255) gctggATAAATTCCCGCGGTTTTTGGCATTCCAACGCCGAACCGATGCGCCAGTTCGCGGCCTTCTTTCTTTTTGCGGTACCCTTCCTCCCCTTGCCCAATTGTCTCCTGCGGGCTTTGTACAGCCATGCCGTGACCGAGCATGTTGCCAAAGCGGGGGTTACGCGACTGGTTGT‑CGGTCCAGGAAAGCCCCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCAcc < 2:969787/235‑1 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTCGTTGT‑CGGGACCGGGAAGCCCCA‑AAACACCAAACCCCCCCCCTCCCACAA‑AGGTGGGCAGGGGC‑‑GCAcccca > 2:143559/1‑236 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑AATCGCCAAGCCCCCCCATGCCCATAA‑AAGG‑GGCAGGGGCG‑GCACCCCg‑c > 1:981734/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGT‑CGGGACAGGGAAGCACCA‑AATCGCCAAGCCCCCCCCTACCCCTAA‑AAGT‑GGCAGGGGCG‑GCACCCAg‑c > 1:172493/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCACTGGGTGT‑CGGGACAGGGAAACACCA‑AAACGCCACACCCCCCCCTGCCCATAC‑AGGC‑GGGAGGGTGG‑GCAccccg > 2:1190638/1‑236 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTGGTTGT‑CGGGACAGGTAATGACCA‑AATCCCCCACACACCCCCAGACCACAA‑AGCA‑GGCAGGGTCG‑GGAccacac > 2:570572/1‑235 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTGGTTGT‑CGGGACAGGGAAGCACCA‑AAGCGCCCAACCCCCCCATGCCCCTAA‑AAGG‑GGGAGGGGCG‑GCAccccg > 1:703181/1‑236 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTCACGCGACTTGTGGT‑AGGTGCACGGCAACACCACAATCCCCAAACCCCCCCA‑GCACCTAA‑AACG‑GGCAGGGTGGCGCAcccca > 2:842522/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGCGACCCCAATGGGTGTCCGGTA‑GGAGAAGCACCC‑CATCGCCCAACCCCGCCCCACCCATAA‑AAGA‑CGCCGGggcg‑gccgccac > 2:763724/1‑232 (MQ=255) aTTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTTCGCGACTGGTTGT‑CGGTACAGGTAAGACCCA‑CATCGCCCAACCCCGCCCTGCCCCTAA‑AAGT‑GGGCGGGGCG‑GCACCCCA‑cgccg > 1:1177600/1‑238 (MQ=255) tCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGGACAGGGAAGCACCA‑AATCGCCAAGCCCCCCCATGCCCCTAA‑AGGT‑GGCAGGGGCG‑GGACCCAC‑CGCCGCAc > 1:831939/1‑238 (MQ=255) ttATTGGCATTTCAACCCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGa < 1:1155246/236‑1 (MQ=255) ttATTGGCATTGCAATCCCGAGGCTATCCGCCGGTGCGCGGACTTCGTTCCTATTGCGGTCGCCTCCCACCACCTGACCAATCTTCCCCGGCGCGCTTTGTACACCCTGGCCCGGACCGAGCACCATGCCAAACCGGCGGTTACGCGACTGTTTTT‑CGTTACAGGTAAGCCCCA‑GACCCCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGAcgc < 2:685783/239‑1 (MQ=255) cattcaaaCGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATGGCGGTAGCCTTCCACCCCCGGACCAATCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGACCGACCATCATGCCAAACCGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACAtt < 2:981734/232‑1 (MQ=255) tCAACGCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGCCCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTcagc < 1:906319/239‑1 (MQ=255) tCAACGCCGAAGCGATGCGCCAGTGCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCACCCCCTGCCCAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑GGGTACAGGTAAGCACCA‑GAGCGCAAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTcagc < 1:260333/239‑1 (MQ=255) tCAACCCCGAGGCGATGCGCCTGTTCGCGGCCTTCTTTCGTATTGCGGTACCCTTCCCCCCCCTGCCCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACGGAGCATCAGGCCAAAAGGGCGGTTCCGCGACTGGTGGT‑CGGTACAGGTAAGCCCCA‑GACCGCCAGGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTcagc < 2:845969/239‑1 (MQ=255) gcAACGCCGAAGCTATGCGCCCTTTCGCGGACTTCTTTCGTATTGCGTTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCAAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTcagc < 1:657570/238‑1 (MQ=255) aCGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTcagccag < 1:917818/239‑1 (MQ=255) ccGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGCCTGGGTGT‑CGGTCCAGGGAAGCACCA‑GAAGCCCAAGCCCCGCCATGCCCCTAA‑AGGT‑GGCAGGGGCG‑GCACCCAC‑GGCCGCCCCCAGACGGCACACTTccacccacc > 2:568568/1‑230 (MQ=255) aaGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑AATCGCCAAACCCCCCCCTGCCCCTAA‑AAGG‑GGCAGGGGCG‑GCACCCCG‑CGCCGCACCCAGACGCGACCTTTCACCCAAcccccc > 1:1153798/1‑236 (MQ=255) aaGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGAATGGGTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGG‑GGCAGGGGCG‑GCACCCAG‑CGCCGCACCAAGAAGCGGCAATTCAGCCAACCCACg > 1:959680/1‑239 (MQ=255) cAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAgcg < 1:884660/239‑1 (MQ=255) cGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGCCTGGGTGT‑CGGTACAGGTAAGCACCA‑CAAAGCCCAACCCCGCCATGCCCCTAA‑AGGG‑GGCAGGGGCG‑GGACCCAG‑CGCCGCCCCAACAAGCGCCAATTTCGCCCGCCCACCGGGGATCAGACCCGCACGCGCACTCGCACCAAAAc > 2:310229/1‑238 (MQ=255) acccTCCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑a < 1:310229/237‑1 (MQ=255) caccacCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCCCCCCTGGTTGT‑CGGTAACGGTAAGCCCCC‑GATCGCCAAGCCCCCCCCTGCCCCTAA‑AGGT‑GGCAGGGGCG‑GCACCCAC‑CGCCGCCCCCCGCCCCGACACTTCAGCCAGCCCCCGGGCGGTCTGCGCCCCCCCCCCATTCGCACCACAACCGAT‑ACCGTCg > 2:593136/1‑238 (MQ=255) accaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGC‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGa < 1:171357/239‑1 (MQ=255) cccccTGCCCAATCTTCCCCTGCCCGCTTTGTCCACCCATGCCCTCACCGAGCACCACCCCAAACCGGCGGTCACGCGCTTGGTTGT‑CGTTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGAc < 2:959680/236‑1 (MQ=255) ccacgttcccAATTTTTCCCGGCGCGTTTGTTAAATCCAGGCCCCGCCCGAGCATAATGCCAAAACGGCGTTTCCGCGACTGTTTGT‑CGGTACAGGTAAGCACCA‑AACCCCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGAc < 2:901853/231‑1 (MQ=255) ggcccATTCTTCCCCTGCGCCCTTTGTACACCCATGCCTGGACCGACCACCAGCCAAAAACGGCGGTTCCGCGCCGGGTTGC‑GGGTACAGGTAAGCCCCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATccc < 2:318426/236‑1 (MQ=255) gccagaTCTTCTCCGGCGCGTTTTGTACACCCATGCCCTGACCGAGCATCAGGCAAAAACGGCGGTTCCGCGACGGGTTGC‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATccc < 1:1106378/233‑1 (MQ=255) tcttttCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCaaa < 1:747130/211‑1 (MQ=255) tcttctCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCCACTGGTTGT‑CGGGACCGGTAAACACCA‑GCTCGCCAAGCCCCCCCATTCCCCTTA‑AGCC‑GGCAACGTGG‑GCACCCCGACGCGCAACCACTACCCGCCATTCCA‑CTACCCCACGCGCGGTCAGCCCCCCACACGCATCCGCCCCCAAACCGCA‑ACACCCGGACATCACCccacca > 2:1188485/1‑239 (MQ=255) tcttctCCTGCGCGCTTTGTACACCCAGGCCCTGCCCGAGCATCATGCCAAAACGGCGTTTACGCGCCTGTTTGC‑CGGTACAGGTAAGCCCCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa < 1:944407/216‑1 (MQ=255) cTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGCCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCCCCA‑GACCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCaa < 1:568568/239‑1 (MQ=255) gcgcgcTTTGTCCACCCTTGCCCTGACCGAGCATCATGCCAAAACGGCGTTTACGCGTCTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATa < 1:963057/239‑1 (MQ=255) cgcgcTTTGTACATCCATGCCCTGACCGAGCATGATGCCAAAACGGCGGTTACGCGACCGGGTGT‑CGGTACAGGCAAGCACCA‑GAACGCCCAACCCCGCCCTCCCCCCAA‑AAGG‑GGCAGGGGCG‑GCACCCAC‑CGCCCCACCAACAAGCCACATTTTCTCCAACCCACGGGGGATCAGCGCCCCACGCCCACTCGCCCCCCAACCACG‑ACCGCCGCACATCCCCCCACCACTAGCacaa > 2:1018894/1‑235 (MQ=255) cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTTT‑CGGTACAGGTAAACACCA‑AAAAGCCCAACCCCCCCACGCCCCTAA‑AGGT‑GGCAGGGGCC‑GCACCCAG‑CGCCGCCCCCACAACCGCCACTTTAGCCACCCCACCGGGGGGCAGCGCCGCACGCGCGCTCGCACCCACACCCAT‑ACCGCCCGCCATCCCCGCCCCCATGGcgctc > 2:1106378/1‑234 (MQ=255) cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑TGGGAAAGGGAAGCACCA‑AATCGCCCAGCCCCCCCCCGCCCCTAA‑AAGG‑GGCGGGGGCG‑GCACCCCG‑CGCCCCCCCACGACGCGCCATTTTAGCCAGCCCCCGGGGGATCAGCGCCGTACGCGCCGTCGCCCCCAAACCCCT‑AACGCCGGGAATCCCCccaccaccgcccata > 2:995549/1‑229 (MQ=255) aTCCATGCCCTGCCCGAGCATCATGCAAAAGCGGCGTTTACTCGACTGGTTGT‑CGGTACGGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCGCcg < 1:520/239‑1 (MQ=255) ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTcagc < 1:167100/145‑1 (MQ=255) ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcgcg < 1:905556/239‑1 (MQ=255) ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcgcg < 1:6381/239‑1 (MQ=255) tGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGGACAAGGAACCACCA‑GAACCCCCAACCCCCCCATGCCCCTAA‑AAGG‑GGGCGGGGCG‑GCACCCAC‑CGCCCCCCCCCGACGCGACAATTATACCACCCCACGCGGGGGCACGGCCCGCAGCGCCCTTGCCCCAAAACCAATAACCCACGG‑CATCCCCGCCCCACAGCGAAGAACGCTTTTCACCCcgcc > 2:815248/1‑238 (MQ=255) cccTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAACCACCA‑GAGCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCgccgcc < 1:914199/239‑1 (MQ=255) cccTGACCGAGCATCATGCCAAAACGGCGGCTTAGCCGCTGGTTGT‑CGGGAAAGGTAAACCCCA‑AAACGCCAAACCCCGCCCTTCCCCTAACAGGG‑GGCGGGGCCG‑GC‑CCCAG‑CGCCGCCACCAGACGCGACCTTTCATCCAACCCCCAGGGGAACACCGCCAGAACCGCCATCCCACCAAAACACAC‑ACCGGCCGCCAAACCCGCACCACCGTGGATAACGTATTTCACCCcgccgc > 2:157676/1‑238 (MQ=255) ccTGCCCGAGCATCAGGCCAAACCGGCGGTTGCGCGACTGTTTGG‑CGGTACGGGTAAGCACAA‑GCCCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCa < 2:851479/239‑1 (MQ=255) tGACCGAGCATAATGCCAAACCGGCGTTGCCGCGACTGGTTGT‑CGGTACAGGAAAGCCCCA‑GACCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCCCCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑CCCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAg < 1:842522/239‑1 (MQ=255) aCCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTTT‑CGGGACAGGGAAGCACCA‑AAACGCCCAACCCCCCCACGCCCCTAA‑AAGG‑GGGCGGGTCG‑GCCCCCCG‑CGCCGCACCCAGACGCGACATTTCAGCCCGCCCACGGGGGATCAGCGCCGGTCGCGCATTCGCCCCCAAACCGGT‑ACCGCCGGGCATCCCCCCCCCCAAGGCCATAAAGTTTTTCTCCCCgccgcccaccg > 2:572207/1‑234 (MQ=255) gcGAGCACCAGGCCAAACCGGCGGTCACGCGGTTGGTTGT‑GGGTCCGGGAAGGCACAA‑GACCGCCAGCCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg < 2:831939/238‑1 (MQ=255) gacaaccatGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa < 1:1190638/233‑1 (MQ=255) gAGCATCATGCCAAAACGGCGTTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa < 1:143559/239‑1 (MQ=255) catcatGCCAAACCGGCGGTTACGCGACTGGTTGG‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGa < 1:331642/186‑1 (MQ=255) catcatGCCAAAACGGCGGTTACGCGGCTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGc < 1:681111/239‑1 (MQ=255) caccaggCCAAAACGGGGGTCCCGCGATGGGTTGT‑CGGTACGGGTAAGCCCCA‑GATCGCCAGCCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGc < 2:172493/233‑1 (MQ=255) ccAAAACGGGGGTTACGCGACTGGTGGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATg < 1:1044119/237‑1 (MQ=255) cggTTACGCGGCTGGTTGT‑CGGTACAAGGAAGCACCA‑AAACGCCAAGCCCCCCCATGCCCCTAA‑AAGG‑GGGAGGGTCG‑GCCCCCAG‑CGCCCCCCCAAGACGCGACATTTCAGCCAGCCCACGGGGGATCAACGCCGTACGCCCATTCGCCCCAAAACCGAT‑ACCGTCGGGCAACCCCCCCCCAATGGCAATAACGTTTTTCACCCCCCCGCCAAGCCGCCCGCCACAGCAAACCGGAtt > 2:824376/1‑239 (MQ=255) cggTTACGCGACTGGTGGT‑CGGTACAGGTAAGCCCCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGAt < 1:824376/238‑1 (MQ=255) tgccGGTAAAGATAAGCCCCA‑GCCCGCCCGCCCCCGCCATGCCCATAA‑GGGG‑GGCAGGGTGT‑GCGCCCAG‑CGCCGCACCAACACGCGACATGTCAGCCAGCCCACGGGTGATCAGCTCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaa < 2:1043258/235‑1 (MQ=255) gTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACtttt > 1:563985/1‑239 (MQ=255) gTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGC‑CCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACtttt > 1:1077633/1‑238 (MQ=255) cACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca > 1:613472/1‑239 (MQ=255) | AGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGT‑CGGTACAGGTAAGCACCA‑GATCGCCAAGCCCCGCCATGCCCATAA‑AGGT‑GGCAGGGTCG‑GCACCCAG‑CGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGAT‑ACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA > NC_000913/3782679‑3783150 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |