Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,769,955 A→G 6.5% pseudogene (8/87 nt) yibU → pseudogene, H repeat‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,769,9550AG 6.5% 202.5 / 2.7 61pseudogene (8/87 nt)yibUpseudogene, H repeat‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (30/27);  new base G (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCC  >  NC_000913/3769700‑3770236
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                         
tGTAGTGATGATAAAAATATAACATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGAATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGa                                                                                                                                                                                                                                                          >  1:300311/1‑289 (MQ=255)
       atgatAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGAaaaa                                                                                                                                                                                                                                                   >  2:53752/1‑289 (MQ=255)
                                                 ccATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGGAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGaa                                                                                                                                                                                                          <  1:113203/288‑1 (MQ=255)
                                                            tCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATatca                                                                                                                                                                                              >  2:980904/1‑289 (MQ=255)
                                                                       cATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCAATGACCCTACATTGTGATCTATAGCTTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCCCTCCTGCTCTTTTTTGCTTAGAGCCGTCGCAGGAGGAACAACTCATCTCATCATTGCGAt                                                                                                                                                                                    >  1:1187218/1‑288 (MQ=255)
                                                                             tgaatgaTAACTTAATTTTATTTAAAAATATATTATTAACTCACTCTCCAGGAGTTTGTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTcgac                                                                                                                                                                              <  2:300311/288‑1 (MQ=255)
                                                                             tgaatgaTAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTcgacg                                                                                                                                                                             <  1:53752/289‑1 (MQ=255)
                                                                                          aaTTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCa                                                                                                                                                                                                                                                               >  2:294908/1‑194 (MQ=255)
                                                                                          aaTTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCa                                                                                                                                                                                                                                                               <  1:294908/194‑1 (MQ=255)
                                                                                             tttatttaAAAATAATTTAATAACTCACTATCCAGGAGTTTTTTTAACTTAGTTTTATAATGAAAACGATAATTAAAAGGGAAGAAGAAAATAATTGTATACATATATTCCATGACCCTTCATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATcc                                                                                                                                                             <  2:1167084/289‑1 (MQ=255)
                                                                                                 tttaAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGAtct                                                                                                                                                                                  >  1:686922/1‑264 (MQ=255)
                                                                                                 tttaAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTGTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGAtct                                                                                                                                                                                  <  2:686922/264‑1 (MQ=255)
                                                                                                                               ggAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGct                                                                                                                                                                                                                                        >  1:391265/1‑180 (MQ=255)
                                                                                                                               ggAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGct                                                                                                                                                                                                                                        <  2:391265/180‑1 (MQ=255)
                                                                                                                                    ttttttAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGagag                                                                                                                        <  1:980904/287‑1 (MQ=255)
                                                                                                                                               aGTTTTATAATGAATACAATAATTAAAAAGGAAGATGAAAATAGTTGTATACATATAGTCCATGACCCTACATTGTAATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAATCCATCATCGCAATAGATGTAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCAGATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAg                                                                                                           <  2:1187218/289‑1 (MQ=255)
                                                                                                                                                           aaTACGATATTTAAAAGTGGATAAGAAAATATTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAGAAAATCCAATGAGAGCACACTCATCCAGAACTAATTAACa                                                                                               <  1:362/289‑1 (MQ=255)
                                                                                                                                                           aaTACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTACATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGACCTTATTAACa                                                                                               >  2:709596/1‑289 (MQ=255)
                                                                                                                                                           aaTACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATATTCTCATCCTTCGGCAGAACAAGACGGATCAAAAATCAAATGAGACCACACTCATCCAGAACTTATTAACa                                                                                               >  2:637208/1‑289 (MQ=255)
                                                                                                                                                                     ttAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGGTCTATAGATTAAATATTTTTTAAATGTGACTGTCGTCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACttat                                                                                                    <  2:77762/274‑1 (MQ=255)
                                                                                                                                                                     ttAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGGTCTATAGATTAAATATTTTTTAAATGTGACTGTCGTCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACttat                                                                                                    >  1:77762/1‑274 (MQ=255)
                                                                                                                                                                              aagaagaaAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGaa                                                                                                         >  1:822710/1‑260 (MQ=255)
                                                                                                                                                                              aagaagaaAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGaa                                                                                                         <  2:822710/260‑1 (MQ=255)
                                                                                                                                                                                              tataCATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATg                                                            <  1:637208/289‑1 (MQ=255)
                                                                                                                                                                                                     atatTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTcgac                                                                                                                                                                              <  1:261913/168‑1 (MQ=255)
                                                                                                                                                                                                     atatTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTcgac                                                                                                                                                                              >  2:261913/1‑168 (MQ=255)
                                                                                                                                                                                                     atatTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTcga                                                                                                                                                                               >  2:716941/1‑167 (MQ=255)
                                                                                                                                                                                                     atatTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTcga                                                                                                                                                                               <  1:716941/167‑1 (MQ=255)
                                                                                                                                                                                                     atatTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGc                                                                 <  2:340127/277‑1 (MQ=255)
                                                                                                                                                                                                     atatTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGc                                                                 >  1:340127/1‑277 (MQ=255)
                                                                                                                                                                                                           cATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACATCTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTaaa                                                                                 >  1:988547/1‑255 (MQ=255)
                                                                                                                                                                                                           cATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACATCTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTaaa                                                                                 <  2:988547/255‑1 (MQ=255)
                                                                                                                                                                                                             tGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATATTAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCtttt                                                                                                                                                                                                                                   >  1:898761/1‑107 (MQ=255)
                                                                                                                                                                                                             tGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAg                                                                                                           >  1:573340/1‑227 (MQ=255)
                                                                                                                                                                                                             tGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAg                                                                                                           <  2:573340/227‑1 (MQ=255)
                                                                                                                                                                                                             tGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTAATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCtttt                                                                                                                                                                                                                                   <  2:898761/107‑1 (MQ=255)
                                                                                                                                                                                                             tGACCCTACATTGTGATCTATAGATTAAATATTTCTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATAGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATCGCCATCCCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTCACATAATGAATATTAAAGAAAAATCATCGAAGTTAATGAAGTAATATGCaaaa                                             >  1:185535/1‑289 (MQ=255)
                                                                                                                                                                                                                   tACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTagag                                                                                                                                                                                                                         >  2:261800/1‑111 (MQ=255)
                                                                                                                                                                                                                   tACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTagag                                                                                                                                                                                                                         <  1:261800/111‑1 (MQ=255)
                                                                                                                                                                                                                   tACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTTCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAAtt                                       >  2:420401/1‑289 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGCGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAACCTAATGAAGTAATATGCAAAAATAATTAGTCCt                                 >  2:725827/1‑288 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGca                                                                                                                      <  1:984153/203‑1 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGca                                                                                                                      >  2:984153/1‑203 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACttatt                                                                                                   >  2:734904/1‑222 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACttatt                                                                                                   <  1:734904/222‑1 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCtt                                >  1:1069262/1‑289 (MQ=255)
                                                                                                                                                                                                                          tgATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCtt                                >  1:453142/1‑289 (MQ=255)
                                                                                                                                                                                                                             tCTATAGATTAAATATTTTTTAAATGGGACGGTAGTCCTTCAGATAATAAAGCCATCATGGCAATAGAAGGAAAAGCACTCCTGCACTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACa                             <  2:1094827/289‑1 (MQ=255)
                                                                                                                                                                                                                               tataGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATg                                                            <  1:1139130/256‑1 (MQ=255)
                                                                                                                                                                                                                               tataGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATg                                                            >  2:1139130/1‑256 (MQ=255)
                                                                                                                                                                                                                                    aTTAAATATTTTTTAAATGTGACTGTCGTCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCACATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAAAAATTAGTCCTTACAAGGAGCa                      >  1:1094827/1‑289 (MQ=255)
                                                                                                                                                                                                                                    aTTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCacac                                                                                                                   <  2:887935/196‑1 (MQ=255)
                                                                                                                                                                                                                                    aTTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCacac                                                                                                                   >  1:887935/1‑196 (MQ=255)
                                                                                                                                                                                                                                      tAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAg                                                                                                                                                                                                                                                  <  2:545942/67‑1 (MQ=255)
                                                                                                                                                                                                                                      tAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAg                                                                                                                                                                                                                                                  >  1:545942/1‑67 (MQ=255)
                                                                                                                                                                                                                                        aaTATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAaataat                                        >  1:1127753/1‑267 (MQ=255)
                                                                                                                                                                                                                                        aaTATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAaataat                                        <  2:1127753/267‑1 (MQ=255)
                                                                                                                                                                                                                                              ttttAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAg                                     <  2:610772/264‑1 (MQ=255)
                                                                                                                                                                                                                                              ttttAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAg                                     >  1:610772/1‑264 (MQ=255)
                                                                                                                                                                                                                                                             tCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGta                                                                                                                                                                        <  2:68766/118‑1 (MQ=255)
                                                                                                                                                                                                                                                             tCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGta                                                                                                                                                                        >  1:68766/1‑118 (MQ=255)
                                                                                                                                                                                                                                                               aTCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATctcccccc  <  2:638453/282‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATctcccccc  >  1:638453/1‑282 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                         
TGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACATAATGAATATTAAAGAAAAATCATCGAAGCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCC  >  NC_000913/3769700‑3770236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: