| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,550,108 | T→C | 5.6% | Q789R (CAG→CGG) | malP ← | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,550,108 | 0 | T | C | 5.6% | 240.9 / 4.3 | 71 | Q789R (CAG→CGG) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (28/39); new base C (2/2); total (30/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
AGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC > NC_000913/3549839‑3550392 | agcTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCg > 2:169226/1‑289 (MQ=255) tatcTTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt < 1:580211/288‑1 (MQ=255) cgcTGCCCTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGACGTTGGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATAGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAACCGCGAATAGGGAGATACGAGCCaaa > 2:294691/1‑289 (MQ=255) cTGCCCTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACa < 1:294691/289‑1 (MQ=255) accaTCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg < 1:207123/289‑1 (MQ=255) ccaTCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcgggc > 2:554905/1‑289 (MQ=255) gCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcg < 1:324413/289‑1 (MQ=255) ttgTATAAACCAGGACTTGCGGGGCTGGGGTTCCCGCCACTTGCGTGTCGGTACGTTGATGCGTCGCGTAAAGAAAACGCCGTTTGGTTTCGGTGCTAATCGGCTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGTTCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcgc < 2:351740/286‑1 (MQ=255) tGGTATAAACCATGACATTCGTGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcgc < 1:584101/288‑1 (MQ=255) tGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcgc < 1:212637/288‑1 (MQ=255) tGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGcct > 1:578201/1‑288 (MQ=255) tGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGcct > 1:354544/1‑288 (MQ=255) gACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGcctc > 2:373490/1‑288 (MQ=255) cATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGcctcct < 1:509522/288‑1 (MQ=255) tCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTTGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAACGCGAACCGAACTAAACATACCCCAGCCGGCGGTATTGAGGAACGCCGCGCTAGTGCCGGCCTGCTggg > 2:126/1‑287 (MQ=255) cGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg < 1:169226/289‑1 (MQ=255) aCTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCgg < 2:578201/288‑1 (MQ=255) tGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTAc > 1:163964/1‑289 (MQ=255) gCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCa > 2:192131/1‑235 (MQ=255) gCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCa < 1:192131/235‑1 (MQ=255) tACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGcac > 1:349562/1‑288 (MQ=255) tACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGcac < 1:568047/288‑1 (MQ=255) cACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCCAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCCCGAATAGAGCGATCCGAGCTAAACATACCCCAGCGGGCGGCATTGAGGATCGCCCCCCGGATCCAGGCCTCCTGGTCGCGGTaaacgcctccccc > 2:320685/1‑276 (MQ=255) aCTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCa > 1:100025/1‑286 (MQ=255) cTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCt > 1:426335/1‑288 (MQ=255) tttCGGGGCGGTACGTTGATGCTGCGCGTCAAGCAAAGGCGGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGc < 2:387853/289‑1 (MQ=255) tCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACACCCACCTGCtt > 1:519439/1‑289 (MQ=255) ggTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGcc < 1:373490/289‑1 (MQ=255) ggTACGTGGATGCAGCGCGTGAAGGAAACGCCGTTTGGTTTGTGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGc < 2:519439/288‑1 (MQ=255) gTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGAGcc < 2:349562/288‑1 (MQ=255) tACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCct < 1:554905/288‑1 (MQ=255) aaaCGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGc < 2:466687/287‑1 (MQ=255) aaCGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCAt < 1:474518/289‑1 (MQ=255) cGCCGTTGGGTTTCGCGCCTAATCGACTGCGGTTTACCGTGGGCCTTGATGTAATTGGGCATAATCGCGGCCGCAGGCGGGGCAGTAGCCAGACGTTTGGTATCCATCGAGCGTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCa < 2:455294/289‑1 (MQ=255) tcggTTTGGTTTCGTCGCTAATCGACTGCGGTTTACCGTCGGCATTGATGTAATTGGAGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTAGTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCAc < 1:560453/286‑1 (MQ=255) cGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCg > 1:232206/1‑289 (MQ=255) gTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGcc < 1:581560/230‑1 (MQ=255) gTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGcc > 2:581560/1‑230 (MQ=255) tttACAGTGGGCATTGGTGTAATTGGGGGTAATCCCCGCCGCCTGCTCGGCATTATCCAGACGTTTGCTTTCCTGCGAGCTTCCTTA‑GCGTTTTGCGTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGGGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGGACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCGCTGTTTGCCGATa < 2:298407/289‑1 (MQ=255) aCCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg > 1:234940/1‑196 (MQ=255) aCCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg < 2:234940/196‑1 (MQ=255) cGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACtgtg < 2:163964/289‑1 (MQ=255) gggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTg > 1:159767/1‑271 (MQ=255) gggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTg < 2:159767/271‑1 (MQ=255) ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCa > 2:84888/1‑245 (MQ=255) ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCa < 1:84888/245‑1 (MQ=255) aTTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGc > 2:231148/1‑263 (MQ=255) aTTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGc < 1:231148/263‑1 (MQ=255) gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCAc < 2:169369/231‑1 (MQ=255) gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCAc > 1:169369/1‑231 (MQ=255) gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCAt > 1:140194/1‑287 (MQ=255) cgccgcCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCg > 2:519953/1‑289 (MQ=255) gccAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGc < 2:426335/289‑1 (MQ=255) cATTAGCCGGACGTTTGCTTTCCGTCGGTCTTCCTGACGCTTTTTGCC‑GCCTGATACGGGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTAc < 2:80935/288‑1 (MQ=255) cGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGGGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGc < 2:140194/288‑1 (MQ=255) tGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCttt > 1:141266/1‑289 (MQ=255) tGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCCGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg > 2:208656/1‑134 (MQ=255) tGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCCGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg < 1:208656/134‑1 (MQ=255) tttCCATCGAGCGTCCTTA‑GCGTTTGGCCTGCCAGATACGGGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCaa < 2:232206/289‑1 (MQ=255) gAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGcc > 1:263970/1‑153 (MQ=255) gAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGcc < 2:263970/153‑1 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCTCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCCCTGTATTTACCGCTTTCCAGCTCTTTCAATCCTGCGTCAAGCCCCTTAtcttt > 2:298675/1‑289 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGc < 2:376290/220‑1 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGc > 1:376290/1‑220 (MQ=255) ttGCCTGCCAGATACGAGCCCGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTAtcttt > 2:44176/1‑289 (MQ=255) tgcctgccAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTAtctt > 2:20297/1‑287 (MQ=255) ctgccAGATACGAGCCCGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttcttc < 1:44176/289‑1 (MQ=255) gccAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCg < 1:320685/289‑1 (MQ=255) gCCTGGTAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAcc < 2:141266/288‑1 (MQ=255) gCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAcc < 1:298675/288‑1 (MQ=255) gCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAcc < 1:519953/288‑1 (MQ=255) | AGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTA‑GCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC > NC_000913/3549839‑3550392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |