Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,718	G→A	6.4%	A600A (GCG→GCA)	rsxC →	SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,718	0	G	A	6.4%	208.7 / 6.0	78	A600A (GCG→GCA)	rsxC	SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base A (3/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

GCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAA  >  NC_000913/1708431‑1709003                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                               gCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGGCCCAAAgcg                                                                                                                                                                                                                                                                                                >  2:728515/1‑288 (MQ=255)   ccAGGATACACAGCAGATTGTGATTAAAGCGGGCGAACGCCCGGATACACGTGGAAGTATTGCAGCACGGGAGGGCCGTAAAGCGCAAGCAAGA‑GCGAAAAAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTAGCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                                                                              <  2:461608/288‑1 (MQ=37)     aGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                                                                                                                                                                                                                                                           >  1:621023/1‑289 (MQ=255)      ggCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCCCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcccgcca                                                                                                                                                                                                                                                                                           >  2:582325/1‑286 (MQ=255)           cacaGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                                                                                                                                                                                                                     >  2:775368/1‑289 (MQ=255)            acaGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCCGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGaa                                                                                                                                                                                                                                                                                    <  1:382046/289‑1 (MQ=255)                ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGc                                                                                                                                                                                                                                                                                >  1:536824/1‑289 (MQ=255)                ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCGGCAACCGc                                                                                                                                                                                                                                                                                >  2:77742/1‑289 (MQ=255)                 cTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCTGTGCCAAAGCGCGCAAGCTGGAACAGCa                                                                                                                                                                                                                                                                               >  1:271244/1‑289 (MQ=255)                 cTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGCCCGTGCCAAACCGCGCCAGCGGGAACAGCa                                                                                                                                                                                                                                                                               >  2:315607/1‑289 (MQ=255)                  taatgggATTAAAGCGGGCGAACGCCCGGATAACAGTGAAATTAGTGGAGCACGGAAAGCCCGAAAAGCGCAAGCCAGC‑GCGAAACAGGAAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                              <  2:461071/283‑1 (MQ=255)                        gATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCTCAACCTGGAACAGCAAACGGCt                                                                                                                                                                                                                                                                        >  2:7644/1‑289 (MQ=255)                        gATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCt                                                                                                                                                                                                                                                                        >  1:699340/1‑289 (MQ=255)                        gATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCCGGAACAGCAACAGGcc                                                                                                                                                                                                                                                                        >  1:53886/1‑288 (MQ=255)                          ttAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCCACGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGCGACCCGCACAAGGCTaa                                                                                                                                                                                                                                                                      >  2:394191/1‑289 (MQ=37)                                   gCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAcc                                                                                                                                                                                                                                                             >  1:187459/1‑289 (MQ=255)                                   gCGAACACGCGGATAACAGTGCAATAATTGGAGAACGGGAAGACCGTAAAACGCAAGCCAGA‑GCGAAACAGGCAGAACGGCAGCAAACTAAAGACGTGGCAACCGGAGCTTATCCACCGAAAACTGCCGTTGAGG‑CAGCTATCGCCCGCGCCGAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc                                                                                                                                                                                                                                                              <  2:271244/288‑1 (MQ=21)                                                     gTGCAATTATTGCAGCGCGGGAAGCCCGTAAAGAGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCCAACTAACGACGCAGCAACCGTCGCTGATCCACGTAATACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CAGGAACAGCAACAGGCTCATGCGGCACCAGAACAACTGGTCCATCCGCGCAAAGCAGCCGCCGCAGCAGCGATTGCCCGTTCTCAGGCGCGAACGCCGGCCCAGCACCAGGCTCTACCGGAGTCAGAAGCACAgctgatc                                                                                                                                                                                                                                             >  2:384539/1‑281 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑cc                                                                                                                                                                                                                                          >  1:441128/1‑289 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAACCGGGAACAGCCACGGGCTAATGCGGAACCCGAAAGACAGGTCGAT‑c                                                                                                                                                                                                                                           >  2:597124/1‑288 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                              <  2:409443/254‑1 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                              >  1:409443/1‑254 (MQ=255)                                                           ttattGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCaa                                                                                                                                                                                                                                    >  1:283291/1‑289 (MQ=255)                                                                gcagcaCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCCAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAgcc                                                                                                                                                                                                                                >  1:615097/1‑288 (MQ=255)                                                                    caCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                                                                              <  2:349386/223‑1 (MQ=255)                                                                    caCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                                                                              >  1:349386/1‑223 (MQ=255)                                                                     aCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTc                                                                                                                                                                                                                          >  1:826498/1‑289 (MQ=255)                                                                      cGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                                                                                                                      <  2:564086/261‑1 (MQ=255)                                                                      cGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                                                                                                                      >  1:564086/1‑261 (MQ=255)                                                                        ggAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGaa                                                                                                                                                                                                                       >  1:348884/1‑289 (MQ=255)                                                                          acacccGTAAAGGGCAAGACAGG‑ACAAAAACGGAAGACCTGCAGAAAACTAACGCGGCCGAAACCCTGGCTGACCCAGGTAAAACTGCCGTTGAAG‑CAGCTATCGCACGGGCCAAAGCGCGGAAG‑CAGGAACAGCAACAGGATAATGCGGAACCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                      <  2:536824/285‑1 (MQ=17)                                                                          aagacgatAAAACGCAGACAAGA‑GCGAAAAGTGCAGAACAAAATGAAAGTAACGACGCAGCAACCGTGGCTGAACCACGAAAAACGGCCGTGGAAG‑CAGATATCACCCGAGGAAAAGCGCGCAAG‑CTGGAACAGCAACAGGCAAATGCGGAACCAGAACAACAGGTCGATCCGCGTAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                      <  2:813086/281‑1 (MQ=17)                                                                          aaGCCCGTAAAGCGAAAGCCAGA‑GCGAAACAGGCAGAACGGCAGCAAACTAAGGACGCAGCAACCGTCGCGGATCCACGTAAAACTGCCGTAGAAG‑CAGCTATCGCCCGGGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAAGCAGCACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                      <  2:361384/288‑1 (MQ=18)                                                                          aaGCCCGCAAAGGGCAAG‑CAGAGGCGAAACAGGGAGAACTGCAGCAAACTAACGACGATACAACGGTGACTAACCCACCAAAAACTGCCGTGGGAG‑CAGCCATGACCGGCGACAAAGCGCGAAAG‑CTGGAACAGCAACAGGATAATACGGAACCAGAACCACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                      <  2:283291/288‑1 (MQ=17)                                                                               cGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTa                                                                                                                                                                                                                <  1:757531/289‑1 (MQ=255)                                                                               cGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTa                                                                                                                                                                                                                <  1:766223/289‑1 (MQ=255)                                                                                  aaaGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTAtt                                                                                                                                                                                                              >  1:361384/1‑288 (MQ=255)                                                                                    aGCGAAAGCAAGA‑GCAAAACAGACAGACCTGAGGGAAACTAACGCCGCAGAACCCCTGGCGGACCCACGTAAAACTGCCGTTGAGG‑CAGCTATCGCCCGGCCCACAGCGGGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc                                                                                                                                                                                                           <  2:699340/289‑1 (MQ=17)                                                                                       gcAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTCGATCCCGCCCAAACCCCCCGTCCAACCCCCTTTTGCCCg                                                                                                                                                                                                         >  1:127434/1‑289 (MQ=21)                                                                                                a‑gCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAAAAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGc                                                                                                                                                                                                >  1:801607/1‑288 (MQ=255)                                                                                                    gAAACAGGCAGAACTGCAGCAAACTAACGACGGAGCACCCGTGGCTGATCCCCGTAAAACTGCCGGACAGG‑CAGCTATCGCACGGGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGcgcg                                                                                                                                                                                             <  2:621023/288‑1 (MQ=14)                                                                                                    gAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                             <  1:352784/288‑1 (MQ=255)                                                                                                    gAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTG‑CCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                             <  1:681613/287‑1 (MQ=255)                                                                                                         aGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                                                                                                                                                       <  1:775368/289‑1 (MQ=255)                                                                                                         aGGAAGAACGGCAGCAAACGAAGTGCAGAGCACCCGGTGCTAACCAAAGTAAAACGGCAGTAGAAGAGAGC‑AGTGCACGAGCAACAGCGCGGAAG‑CTGGAACAACAACAGGCTAATGCGGAACCAGAAGAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                                                                                                                                                       <  2:813787/289‑1 (MQ=11)                                                                                                            cAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc                                                                                                                                                                                        <  1:869199/285‑1 (MQ=255)                                                                                                             aGAACGGCAGCAAAGTAACGGCGCAGGAACAGTAGATGAACCACGAAAAATTGCCGATGAAG‑CAGCTATCGCACGCGCCGAAGCGCGCAAG‑CTGGAACAACAACAGGCTAATGCCGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                                   <  2:615097/289‑1 (MQ=17)                                                                                                                   gcagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                             <  1:692141/289‑1 (MQ=255)                                                                                                                   gcagcaAACTAACGACGCAGCAACCCTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                             <  1:7644/289‑1 (MQ=255)                                                                                                                              aggaagcagcaAACGTTCATAATCAACGTAAAACTGCAGTTGAAG‑AAGCTAGCACCCGCGACAAAGCGCGAAAG‑CTGGAACAGCGACAGGCTAATGCGGAAGCAGAACAAGAGGTCGATCCGCGGAAAGCTGCCGGCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                                  <  2:826498/284‑1 (MQ=17)                                                                                                                                  cgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                                                                                                                                               <  1:582325/288‑1 (MQ=255)                                                                                                                                       aagcggtgagtGATCGACGAATAACTGACGTTGACG‑CAGCTATCGCCGGGGCCAACGCG‑GCGAGTCTGGACCAGAGACAGGCTAATGCGTAAGCGGAACACCTGGCCGATCCGCGTAAAGCTGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAGGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                                                                                                                                                          <  2:349672/278‑1 (MQ=17)                                                                                                                                        aaCCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa                                                                                                                                                        <  1:412003/289‑1 (MQ=255)                                                                                                                                          ccGTTGCTGATCCACGTAAAACTGACGTTGAAG‑CAGCTATCGACCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                                      <  1:766247/289‑1 (MQ=255)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                                                                                                                                                 >  1:853410/1‑289 (MQ=255)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAT‑CCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaa                                                                                                                                                                                                   <  1:438914/239‑1 (MQ=11)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAT‑CCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaa                                                                                                                                                                                                   >  2:438914/1‑239 (MQ=11)                                                                                                                                                aagaacgaggTAAAAGTGCCGTGGATG‑CGGCTATTGACGAGGCCACAGCGCGCAAG‑CCGGCACAGCCACAGGCTAAGGAGGAACCAGAAGAGAAGGTCGGTCCGCGAAAAGCTGCGGTCGAAGCCGCTAGTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                                                                                                                                                 <  2:243464/279‑1 (MQ=14)                                                                                                                                                    tCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                                                                            <  1:736891/289‑1 (MQ=255)                                                                                                                                                     ccACGTATAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt                                                                                                                                                >  1:349672/1‑284 (MQ=255)                                                                                                                                                       aCGAAAAACGACCGTCGAAG‑AAGCGAGCGCCCGCTCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACGGGTCGATCCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAANAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                                                                         <  1:70466/289‑1 (MQ=14)                                                                                                                                                        cGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCACAGCCCCACAGCTGGAACAGCAACCGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                                                                         >  1:781633/1‑288 (MQ=255)                                                                                                                                                        cGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCAGGAACAGCAACAAGCTAATGCCGCGCCAGAACAACAGGTCGATcgg                                                                                                                                         >  1:223549/1‑286 (MQ=255)                                                                                                                                                                                      ccGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                            <  1:480518/288‑1 (MQ=255)                                                                                                                                                                                         cgcCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGa                                                                                                        >  1:86790/1‑289 (MQ=255)                                                                                                                                                                                                      aaG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc                                                                                                                                                                                                                                                              <  2:320575/127‑1 (MQ=40)                                                                                                                                                                                                            ggAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCGAGAAGAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAACCCGCTCTTGCCCGAGCCAAAGCGCGCACACgg                                                                                      >  1:176637/1‑289 (MQ=255)                                                                                                                                                                                                              aaCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGa                                                                                                                                                                                                                                                                <  2:176516/118‑1 (MQ=39)                                                                                                                                                                                                                 aGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACa                                                                                                                                                                                                                                                    >  1:320575/1‑127 (MQ=255)                                                                                                                                                                                                                       gggCAAATGCGGACCCAGCAACACAGGCCGTACCGCGTAAAGCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCaa                                                                             <  1:94443/286‑1 (MQ=14)                                                                                                                                                                                                                        ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                                                                                                                      >  1:176516/1‑118 (MQ=255)                                                                                                                                                                                                                                  gAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCgg                                                                >  1:583110/1‑289 (MQ=255)                                                                                                                                                                                                                                   aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCa                                                                                                                                                                                                                                                                               >  2:482743/1‑82 (MQ=38)                                                                                                                                                                                                                                   aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCa                                                                                                                                                                                                                                                                               <  1:482743/82‑1 (MQ=38)                                                                                                                                                                                                                                        gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCCAAGCCCGCAAACCGGACCAGCAACCGGCTAATGCCGAGCCCGAACAACAGGTCGAACCACCCAAAGCCGCAGTTGCACCCCCTATTGCCCGCACCAAAGCCCACAAACGGGCACCGCAACCGGCCGATGCGGAACCaa                                                          >  2:817989/1‑288 (MQ=14)                                                                                                                                                                                                                                                     aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGa                                             >  1:782116/1‑289 (MQ=255)                                                                                                                                                                                                                                                      tCCGGGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGATAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAt                                            <  1:597124/289‑1 (MQ=255)                                                                                                                                                                                                                                                                    ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCACGCAAAGCTg                               >  1:134888/1‑288 (MQ=255)                                                                                                                                                                                                                                                                           agcagcCATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                        <  1:315607/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                 tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCt                 >  1:710184/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                         gggCCAAAGCGCGCAAGCGGGAACAGCAACAGGCAAAAGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcg         <  1:618922/287‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGCGCAAACGGGCACCGCAACCGGCTCATGCGGACCCAGATGAACCGGTTGACCCGCGCAAAGCTGCCGTCGCAGCCGCTATCGCAccc        >  1:865824/1‑286 (MQ=14)                                                                                                                                                                                                                                                                                                aGCGCGCAAGCTGGAACAGCAACAGGCTAATGAGGAACCAGAACGACAGGTCGAT‑CCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaaa  <  1:77742/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                               GCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAA  >  NC_000913/1708431‑1709003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑