Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,634,3440CT52.0% 1.2 / 23.5 25F41F (TTC→TTT) dtpBdipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (5/7);  new base T (13/0);  total (18/7)
Fisher's exact test for biased strand distribution p-value = 1.65e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAATCTATTCACCGCATCAATATTAAGAATCTCTGACAGATGTAAACTTTTTCGCGCGTTATCCCTTACGCGTTCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGT  >  CP009273/3634110‑3634571
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   
aTTAATCTATTCACCGCATCAATATTAAGAATCTCTGACAGATGTAAACTTTTTCGCGCGTTATCCCTTACGCGTTCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTaa                                                                                                                                                                                                                                <  2:191898/240‑1 (MQ=255)
            aCCGCATCAATATTAAGAATCTCTGACAGATGTAAACTTTTTCGCGCGTTATCCCTTACGCGTTCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGAtt                                                                                                                                                                                                                    <  1:417647/240‑1 (MQ=255)
              cGCATCAATATTAAGAATCTCTGACAGATGTAAACTTTTTCGCGCGTTATCCCTTACGCGTTCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGtt                                                                                                                                                                                                                                  >  1:2728/1‑224 (MQ=255)
                                                                         gTTCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAACAGCTTTGa                                                                                                                                                                                                                      >  1:148300/1‑177 (MQ=255)
                                                                          ttCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTtaaacgc                                                                                                                                                                                                                           >  1:233077/1‑167 (MQ=255)
                                                                                           ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCCTTtaa                                                                                                                                                                                                                               >  1:103500/1‑150 (MQ=255)
                                                                                                  gAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGacc                                                                                                                              <  1:332815/240‑1 (MQ=255)
                                                                                                    aGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGaccac                                                                                                                            <  1:169141/240‑1 (MQ=255)
                                                                                                     ggTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCTTTAAAc                                                                                                                                                                                                                              >  2:220952/1‑141 (MQ=255)
                                                                                                       ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTtttttc                                                                                                                                                                                                                                     >  1:32820/1‑132 (MQ=255)
                                                                                                       ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAACTTGGGTtttc                                                                                                                                                                                                                 >  2:169141/1‑152 (MQ=255)
                                                                                                       ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTaaa                                                                                                                                                                                                                               >  1:390796/1‑138 (MQ=255)
                                                                                                       ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTTTTTAACa                                                                                                                                                                                                                             >  1:63312/1‑140 (MQ=255)
                                                                                                         aataaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTaaa                                                                                                                                                                                                                               >  1:150640/1‑136 (MQ=255)
                                                                                                                             cacCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTtaaaa                                                                                                                                                                                                                             >  1:254966/1‑116 (MQ=255)
                                                                                                                                  aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTTTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTCTTTGTTTAACAGCTTTGATTTTCGc                                                                                                                                                                                                               >  2:230818/1‑127 (MQ=255)
                                                                                                                                  aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTGTTTAACaac                                                                                                                                                                                                                           >  1:183935/1‑113 (MQ=255)
                                                                                                                                  aTGGGGATGCTGCAGCAACCTCGCCCA‑TTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTTTTAAAAAACTTGGGTTTTCGCaa                                                                                                                                                                                                             >  1:345208/1‑128 (MQ=255)
                                                                                                                                          gctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTGTTTAACACCTTgg                                                                                                                                                                                                                       >  2:142605/1‑111 (MQ=255)
                                                                                                                                            tgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTaaa                                                                                                                                                                                                                               >  1:262197/1‑101 (MQ=255)
                                                                                                                                               agcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGCGCAGGGCGTACTGGCGGCTTTTTTTGTTCAACAg                                                                                                                                                                                                                            >  2:214967/1‑101 (MQ=255)
                                                                                                                                                     cTCGCCCATTTTTCATGATCTGTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGa                                                                           <  1:341518/240‑1 (MQ=255)
                                                                                                                                                                                             gCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAACAGCTTgg                                                                                                                                                                                                                       >  2:199721/1‑60 (MQ=255)
                                                                                                                                                                                                              gCGTGCAGGGCGTACTGGCGGTTTTCTTTGCTAAACAGCTTTGATTCTCGCaa                                                                                                                                                                                                             >  1:125462/1‑53 (MQ=255)
                                                                                                                                                                                                                  gCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTggg              <  2:290554/240‑1 (MQ=255)
                                                                                                                                                                                                                              ggCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGt  <  1:358717/240‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   
ATTAATCTATTCACCGCATCAATATTAAGAATCTCTGACAGATGTAAACTTTTTCGCGCGTTATCCCTTACGCGTTCATACTTTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGT  >  CP009273/3634110‑3634571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑