BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP025533	2,442,561	Δ1 bp	pseudogene (54/2037 nt)	CYL77_11575 ←	peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	2,442,558	0	A	.	99.4%	860.9 / ‑3.7	173	pseudogene (57/2037 nt)	CYL77_11575	peptidase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (80/92); total (80/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

ATGCGCGGAGAGTTTCGGCGCGATAGTGGCTGCGATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCGAAAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACCGGTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGC‑AAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGCAAGCACGA  >  CP025533/2442311‑2442801

    gcgGAGAGTTTCGGCGCGATAGTGGCTGCGATTTCATCCCTTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAGTGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCTGGCGGGAAATCAGCCACCTTGATCGCTGCTaaatcc                                                                                                                                                                                                                                                 >  2:1152594/1‑246 (MQ=255)

    gcgGAGAGTTTCGGCGCGATAGTGGCTGCGATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCGgaatcg                                                                                                                                                                                                                                                 >  1:1122398/1‑246 (MQ=255)

        agagTTTCGGCGCGATAGTGGCTGCGATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGcn                                                                                                                                                                                                                                            >  2:561129/1‑248 (MQ=255)

               cGGCGCGATAGTGGCTGCGATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGtn                                                                                                                                                                                                                                     >  2:326765/1‑248 (MQ=255)

                       tAGTGGCTGCGATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAgg                                                                                                                                                                                                                             >  1:1218501/1‑249 (MQ=255)

                                 gATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCtt                                                                                                                                                                                                                   >  1:386524/1‑249 (MQ=255)

                                 gATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCtt                                                                                                                                                                                                                   >  1:1349332/1‑249 (MQ=255)

                                      cATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGcn                                                                                                                                                                                                              >  1:260101/1‑248 (MQ=255)

                                       aTCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAg                                                                                                                                                                                                             <  1:972075/249‑1 (MQ=255)

                                       aTCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAg                                                                                                                                                                                                             >  1:573521/1‑249 (MQ=255)

                                          cATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGgtg                                                                                                                                                                                                          <  2:371581/249‑1 (MQ=255)

                                             ttCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTc                                                                                                                                                                                                       >  1:263980/1‑249 (MQ=255)

                                                aGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAAc                                                                                                                                                                                                    >  1:1395499/1‑249 (MQ=255)

                                                   ggaggaATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGt                                                                                                                                                                                                 >  1:1052048/1‑249 (MQ=255)

                                                      ggaATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCAt                                                                                                                                                                                              >  1:3252/1‑249 (MQ=255)

                                                       gaATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGCGCAGGTGTTCAACAGTCAtn                                                                                                                                                                                             >  2:1125442/1‑248 (MQ=255)

                                                       gaATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCAtg                                                                                                                                                                                             <  1:771909/249‑2 (MQ=255)

                                                        aaTCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTCGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAg                                                                                                                                                                                            >  1:885443/1‑249 (MQ=255)

                                                          tCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTg                                                                                                                                                                                          <  1:940729/249‑1 (MQ=255)

                                                          tCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTg                                                                                                                                                                                          <  1:332249/249‑1 (MQ=255)

                                                             gTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGAcc                                                                                                                                                                                       >  1:708746/1‑249 (MQ=255)

                                                              tGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCg                                                                                                                                                                                      <  2:704374/249‑1 (MQ=255)

                                                                      aaGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGt                                                                                                                                                                              >  2:1544510/1‑249 (MQ=255)

                                                                       aGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGtt                                                                                                                                                                             <  1:815077/249‑1 (MQ=255)

                                                                       aGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGtt                                                                                                                                                                             <  2:84709/249‑1 (MQ=255)

                                                                       aGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCGCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGtt                                                                                                                                                                             <  2:171318/249‑1 (MQ=255)

                                                                           gaagaaTACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCtn                                                                                                                                                                         >  1:827560/1‑248 (MQ=255)

                                                                            aagaaTACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTag                                                                                                                                                                        <  2:464655/249‑1 (MQ=255)

                                                                             agacTAGGGCGGCGACGGGGTTGAGGGACAGGTCTGCGCGTTCCAGGGCCTCAACGGTGTTCTCGCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTaga                                                                                                                                                                       <  1:913555/249‑1 (MQ=255)

                                                                              gaaTACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTagag                                                                                                                                                                      <  2:1300748/249‑1 (MQ=255)

                                                                                aTACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGtt                                                                                                                                                                    >  2:1054013/1‑249 (MQ=255)

                                                                                aTACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGtt                                                                                                                                                                    >  2:1462589/1‑249 (MQ=255)

                                                                                 tACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGttt                                                                                                                                                                   <  1:1267242/249‑1 (MQ=255)

                                                                                  aCGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGAGACTAGTTGCTAGAGtttt                                                                                                                                                                  >  2:1046759/1‑249 (MQ=255)

                                                                                   cggcggCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTtc                                                                                                                                                                 >  1:594857/1‑249 (MQ=255)

                                                                                          gACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGt                                                                                                                                                          >  2:640129/1‑249 (MQ=255)

                                                                                           aCGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTc                                                                                                                                                         <  2:1398882/249‑1 (MQ=255)

                                                                                               ggTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGcc                                                                                                                                                     >  1:445692/1‑249 (MQ=255)

                                                                                                     gggACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACctct                                                                                                                                               <  2:876276/249‑1 (MQ=255)

                                                                                                          aGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGt                                                                                                                                          <  1:298305/249‑1 (MQ=255)

                                                                                                           ggCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTc                                                                                                                                         >  2:111217/1‑249 (MQ=255)

                                                                                                              cTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCAcc                                                                                                                                      >  1:1402220/1‑249 (MQ=255)

                                                                                                               ngcgcGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACCg                                                                                                                                     <  1:1225039/248‑1 (MQ=255)

                                                                                                                  gcgTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACCgttg                                                                                                                                  <  1:802088/249‑4 (MQ=255)

                                                                                                                    gTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAGTTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGaa                                                                                                                               >  2:1468146/1‑249 (MQ=255)

                                                                                                                     ttCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAAt                                                                                                                              <  1:1204517/249‑1 (MQ=255)

                                                                                                                       ccAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGc                                                                                                                            <  1:640758/249‑1 (MQ=255)

                                                                                                                          gggCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCAGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATGGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATGATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCcg                                                                                                                         >  1:695359/1‑249 (MQ=255)

                                                                                                                            gCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCcggc                                                                                                                       >  2:941710/1‑249 (MQ=255)

                                                                                                                            gCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCcggc                                                                                                                       >  2:934393/1‑249 (MQ=255)

                                                                                                                               ncAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCggg                                                                                                                    <  1:1315942/248‑1 (MQ=255)

                                                                                                                                  aTGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAAc                                                                                                                 <  1:1377732/249‑1 (MQ=255)

                                                                                                                                    ggTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCa                                                                                                               >  1:834185/1‑249 (MQ=255)

                                                                                                                                         tctcCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCAcc                                                                                                          <  1:1388664/249‑1 (MQ=255)

                                                                                                                                          ctcCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCg                                                                                                         >  2:1095574/1‑249 (MQ=255)

                                                                                                                                          ctcCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCg                                                                                                         >  1:728355/1‑249 (MQ=255)

                                                                                                                                                 gTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGcc                                                                                                  <  2:1574945/249‑1 (MQ=255)

                                                                                                                                                 gTAGGAGCGCCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCGTGAACGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGcc                                                                                                  <  2:199790/249‑1 (MQ=255)

                                                                                                                                                       gCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCatta                                                                                            <  2:959657/249‑1 (MQ=255)

                                                                                                                                                         gTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCattatt                                                                                          <  1:487580/249‑1 (MQ=255)

                                                                                                                                                                ttGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTc                                                                                   >  2:1320925/1‑249 (MQ=255)

                                                                                                                                                                    tAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGAtt                                                                               >  2:1086250/1‑249 (MQ=255)

                                                                                                                                                                    tAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGAtt                                                                               <  2:659192/249‑1 (MQ=255)

                                                                                                                                                                    tAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGAtt                                                                               <  2:145131/249‑1 (MQ=255)

                                                                                                                                                                        gATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCt                                                                           >  1:352447/1‑249 (MQ=255)

                                                                                                                                                                        gATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCt                                                                           <  1:666229/249‑1 (MQ=255)

                                                                                                                                                                            gCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGc                                                                       >  2:767044/1‑249 (MQ=255)

                                                                                                                                                                            gCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGc                                                                       <  2:1208797/249‑1 (MQ=255)

                                                                                                                                                                                     tCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGgcgcg                                                              >  1:880711/1‑249 (MQ=255)

                                                                                                                                                                                         cATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCg                                                          >  2:532698/1‑249 (MQ=255)

                                                                                                                                                                                          aTCGTGTTCAGCTAATGCGAGTTCGAAGGCGGCCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGCGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTCTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCGGCCCGACTCCCTACGCAAAGGCGCGCCCGt                                                         >  1:1423198/1‑249 (MQ=255)

                                                                                                                                                                                               gtTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCt                                                    >  2:1591097/1‑249 (MQ=255)

                                                                                                                                                                                                ttCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTg                                                   <  2:190363/249‑1 (MQ=255)

                                                                                                                                                                                                 tCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGa                                                  >  1:275925/1‑249 (MQ=255)

                                                                                                                                                                                                  cAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGAt                                                 >  1:1587088/1‑249 (MQ=255)

                                                                                                                                                                                                    gCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGc                                               >  2:826417/1‑249 (MQ=255)

                                                                                                                                                                                                    gCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGc                                               >  1:1337723/1‑249 (MQ=255)

                                                                                                                                                                                                       aaTGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGa                                            >  1:951226/1‑249 (MQ=255)

                                                                                                                                                                                                        aTGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGaa                                           <  1:41941/249‑1 (MQ=255)

                                                                                                                                                                                                          gCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGaaaa                                         <  1:1527781/249‑1 (MQ=255)

                                                                                                                                                                                                          gCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGaaaa                                         <  1:876074/249‑1 (MQ=255)

                                                                                                                                                                                                               ttCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAg                                    <  2:445692/249‑1 (MQ=255)

                                                                                                                                                                                                                tCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGa                                   <  1:572508/249‑1 (MQ=255)

                                                                                                                                                                                                                 tgAAGGCGGGCCGGAAATCAGCCACCTTGATCGCTGCGAAAA‑CGGGCAGCTTGTAGGGCAAAGTTGTTGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGaa                                  <  2:830223/248‑1 (MQ=255)

                                                                                                                                                                                                                         ggcgggAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTgcg                          <  1:1080459/249‑1 (MQ=255)

                                                                                                                                                                                                                         ggcgggAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTgcg                          <  2:1550881/249‑1 (MQ=255)

                                                                                                                                                                                                                              gAAATCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTg                     >  2:876824/1‑249 (MQ=255)

                                                                                                                                                                                                                               aaaTCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGa                    >  1:1168732/1‑249 (MQ=255)

                                                                                                                                                                                                                               aaaTCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGa                    <  2:1023271/249‑1 (MQ=255)

                                                                                                                                                                                                                                 aTCAGCCACCTTGCTCGGTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCAGCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATg                  <  2:1497639/249‑1 (MQ=255)

                                                                                                                                                                                                                                 aTCAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGAAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATg                  <  1:455080/249‑1 (MQ=255)

                                                                                                                                                                                                                                   cAGCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCg                >  1:542914/1‑249 (MQ=255)

                                                                                                                                                                                                                                     gCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGAc              >  1:917084/1‑249 (MQ=255)

                                                                                                                                                                                                                                     gCCACCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGAc              <  1:1243191/249‑1 (MQ=255)

                                                                                                                                                                                                                                        aCCTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCgg           <  1:1602423/249‑1 (MQ=255)

                                                                                                                                                                                                                                         ccTTGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGc          <  2:1522936/249‑1 (MQ=255)

                                                                                                                                                                                                                                            tGATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGCAAg       <  2:3252/249‑1 (MQ=255)

                                                                                                                                                                                                                                             gATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGCAAGc      >  2:593396/1‑249 (MQ=255)

                                                                                                                                                                                                                                             gATCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGCAAGc      >  1:1134725/1‑249 (MQ=255)

                                                                                                                                                                                                                                               tCGCTGCG‑AAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACC‑GTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGCAAAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGCAAGCAc    <  2:1168732/249‑1 (MQ=255)

ATGCGCGGAGAGTTTCGGCGCGATAGTGGCTGCGATTTCATCCATTTCAGGGGAGGAATCGGTGCCCTGCAAGTTGAAGAATACGGCGGCGACGCGGTTGAGGGACAGGCCTGCGCGTTCCAGGGCCTCAATGGTGTTCTCCCAGGTAGGAGCGTCCTCATTGGTAGCGATCGCTGTAATTTCAGCATCGTGTTCAGCTAATGCGAGTTCGAAGGCGGGCGGGAAATCAGCCACCTTGATCGCTGCGAAAATCGGGCAGCTGGTAGGGCAAGGTGCTGGGCTTGAGCAGGTGTTCAACAGTCATAGTGACCGATACTAGTTGCTAGAGTTTTCATCATGTCTGCCACCTCTTCCGTCACCGGTTGAATGCCCGGCGGGAACCATCACCGGCGAGCCCCATTATTTCCGCTCGATTCCCTACGC‑AAGGCGCGCCCGTTTGCTGATGCCGAAAAGCTAGAACCCCTGCGCATTGATGCGACCGGCAAGCACGA  >  CP025533/2442311‑2442801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`