Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 2,565,429 Δ1 bp intergenic (‑91/+27) CYL77_12135 ← / ← CYL77_12140 trigger factor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0255332,565,4280T.99.4% 823.0 / ‑3.3 166intergenic (‑90/+28)CYL77_12135/CYL77_12140trigger factor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (94/71);  total (94/72)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

AGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑AAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGTTCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCATCTGAAAA  >  CP025533/2565182‑2565671
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
aGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGtc                                                                                                                                                                                                                                                    <  2:1334234/249‑2 (MQ=255)
  cTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGtcgt                                                                                                                                                                                                                                                  >  1:975861/1‑246 (MQ=255)
   tGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGtcgtc                                                                                                                                                                                                                                                 >  1:1058104/1‑245 (MQ=255)
   tGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGtcgtc                                                                                                                                                                                                                                                 >  1:1381015/1‑245 (MQ=255)
     aCGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCg                                                                                                                                                                                                                                              <  1:147846/249‑1 (MQ=255)
       gcgGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGATCGATCTCTGGGTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGaa                                                                                                                                                                                                                                            <  1:920881/249‑1 (MQ=255)
        cgGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGaaa                                                                                                                                                                                                                                           <  2:1591179/249‑1 (MQ=255)
           tGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCg                                                                                                                                                                                                                                        <  1:199771/249‑1 (MQ=255)
              cTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGc                                                                                                                                                                                                                                     >  1:510751/1‑249 (MQ=255)
                tGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTc                                                                                                                                                                                                                                   >  1:1032433/1‑249 (MQ=255)
                        cGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCan                                                                                                                                                                                                                           >  2:1457244/1‑248 (MQ=255)
                           aaaCCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTc                                                                                                                                                                                                                        >  2:1475692/1‑249 (MQ=255)
                                        ggACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGAt                                                                                                                                                                                                           >  1:658485/1‑249 (MQ=255)
                                         gACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGAta                                                                                                                                                                                                          >  1:714827/1‑248 (MQ=255)
                                            ttGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGa                                                                                                                                                                                                       >  1:1286310/1‑249 (MQ=255)
                                             tgctgcGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGaa                                                                                                                                                                                                      >  1:851249/1‑249 (MQ=255)
                                                ngcgcTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCt                                                                                                                                                                                                   <  2:1596875/248‑1 (MQ=255)
                                                   gcTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCg                                                                                                                                                                                                >  1:1280287/1‑249 (MQ=255)
                                                     nagagCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGAc                                                                                                                                                                                              <  2:1545442/248‑1 (MQ=255)
                                                      agagCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGAcc                                                                                                                                                                                             >  2:93208/1‑249 (MQ=255)
                                                       gagCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGAccc                                                                                                                                                                                            >  2:123092/1‑249 (MQ=255)
                                                            gcgTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGc                                                                                                                                                                                       >  2:713109/1‑249 (MQ=255)
                                                             cgTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCt                                                                                                                                                                                      >  2:1416943/1‑249 (MQ=255)
                                                              gTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTc                                                                                                                                                                                     <  2:988509/249‑1 (MQ=255)
                                                              gTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTc                                                                                                                                                                                     >  1:711068/1‑249 (MQ=255)
                                                                 tGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCGCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCa                                                                                                                                                                                  <  1:373193/249‑1 (MQ=255)
                                                                       gTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAg                                                                                                                                                                            <  2:872525/249‑1 (MQ=255)
                                                                                tGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTAc                                                                                                                                                                   >  1:1575305/1‑249 (MQ=255)
                                                                                nggCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTAc                                                                                                                                                                   <  2:132916/248‑1 (MQ=255)
                                                                                nggCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTAc                                                                                                                                                                   <  2:87807/248‑1 (MQ=255)
                                                                                    ttcagttcagaAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAg                                                                                                                                                               >  2:1366072/1‑249 (MQ=255)
                                                                                      cagttcagaAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCt                                                                                                                                                             >  1:588045/1‑249 (MQ=255)
                                                                                        gttcagaAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTgc                                                                                                                                                           >  1:1052980/1‑249 (MQ=255)
                                                                                              aaaaTGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACg                                                                                                                                                     >  2:59438/2‑249 (MQ=255)
                                                                                               aaaTGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGc                                                                                                                                                    >  1:796834/1‑249 (MQ=255)
                                                                                                aaTGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGcc                                                                                                                                                   <  2:1483473/249‑1 (MQ=255)
                                                                                                 aTGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGccc                                                                                                                                                  <  2:867626/249‑1 (MQ=255)
                                                                                                   ggAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCg                                                                                                                                                <  1:1562780/249‑1 (MQ=255)
                                                                                                    gtaCCTCAACGGTGAACTCTGAACGGGTGTCGCTCAGCTTCTCGACATAACTCTTCACCAGCAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACTAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGa                                                                                                                                               <  1:668381/247‑1 (MQ=255)
                                                                                                    gAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGa                                                                                                                                               <  1:460653/249‑1 (MQ=255)
                                                                                                     aaCCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGat                                                                                                                                              >  2:1161177/1‑249 (MQ=255)
                                                                                                     aaCCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGat                                                                                                                                              >  1:1148182/1‑249 (MQ=255)
                                                                                                     aaCCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGat                                                                                                                                              >  2:164525/1‑249 (MQ=255)
                                                                                                             cGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAAtt                                                                                                                                      <  2:1389236/249‑1 (MQ=255)
                                                                                                                tGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGa                                                                                                                                   >  1:146411/1‑249 (MQ=255)
                                                                                                                   gcTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGAccc                                                                                                                                <  2:1124874/248‑1 (MQ=255)
                                                                                                                              gtgtCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTa                                                                                                                     <  2:1169927/249‑1 (MQ=255)
                                                                                                                                gtCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAac                                                                                                                   <  2:332682/249‑1 (MQ=255)
                                                                                                                                      cAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACtg                                                                                                             <  2:1527444/249‑1 (MQ=255)
                                                                                                                                      cAGCTTCTCGAAAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACtg                                                                                                             <  1:660923/249‑1 (MQ=255)
                                                                                                                                          ntctcGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGAt                                                                                                         <  1:212469/248‑1 (MQ=255)
                                                                                                                                            nncGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGAtct                                                                                                       <  2:1261604/247‑1 (MQ=255)
                                                                                                                                            ctcGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGAtct                                                                                                       >  1:910885/1‑249 (MQ=255)
                                                                                                                                              cGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCa                                                                                                     <  2:1247431/249‑1 (MQ=255)
                                                                                                                                                aCAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCCGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACAGAGCTCGTCCTCACGTCGGGGCGACAGGATTTGAACCTGCGACTCACTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGa                                                                                                   >  1:574255/1‑249 (MQ=255)
                                                                                                                                                 cAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGaa                                                                                                  <  2:45621/249‑1 (MQ=255)
                                                                                                                                                   gAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACa                                                                                                >  2:506581/1‑249 (MQ=255)
                                                                                                                                                    aaCTCTTCACGAGTAAATCTCCTGGATGATCGGGGAAGGGGAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGGTTAATGCGGTTTTTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTTGGCCACGCCCCGATATTTAAATTTACCCCTCGAATACTAAAACACCGTGGTCCCCGAaaaa                                                                                               >  2:418699/1‑249 (MQ=255)
                                                                                                                                                    aaCTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACaa                                                                                               <  2:806801/249‑1 (MQ=255)
                                                                                                                                                     aCTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACaaa                                                                                              >  1:1183182/1‑249 (MQ=255)
                                                                                                                                                        ctTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTc                                                                                           >  1:1324435/1‑249 (MQ=255)
                                                                                                                                                                 tAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGa                                                                                  <  2:1195036/249‑1 (MQ=255)
                                                                                                                                                                  aaaTCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGAc                                                                                 >  1:1390677/1‑249 (MQ=255)
                                                                                                                                                                       nccTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACt                                                                            <  2:1136957/248‑1 (MQ=255)
                                                                                                                                                                        ccTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATCAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTa                                                                           >  1:313596/1‑249 (MQ=255)
                                                                                                                                                                             atgatCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGtct                                                                      <  2:329516/249‑1 (MQ=255)
                                                                                                                                                                              tgatCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGtctc                                                                     >  1:331066/1‑249 (MQ=255)
                                                                                                                                                                                  cGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCtgtg                                                                 >  2:270578/1‑249 (MQ=255)
                                                                                                                                                                                    ggaaagggaaagTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCt                                                               >  1:365343/1‑249 (MQ=255)
                                                                                                                                                                                     gaaagggaaagTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTg                                                              >  2:1419324/1‑249 (MQ=255)
                                                                                                                                                                                          ggaaagTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAAccc                                                         >  2:616931/1‑249 (MQ=255)
                                                                                                                                                                                          ggaaagTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAAccc                                                         <  1:1515663/249‑1 (MQ=255)
                                                                                                                                                                                                tttCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTgc                                                   >  1:386224/1‑249 (MQ=255)
                                                                                                                                                                                                   cAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCt                                                <  2:110247/249‑1 (MQ=255)
                                                                                                                                                                                                     gATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCtgt                                              >  2:1546426/1‑249 (MQ=255)
                                                                                                                                                                                                      atatTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCtgtg                                             >  1:877007/1‑249 (MQ=255)
                                                                                                                                                                                                      atatTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCtgtg                                             >  2:886665/1‑249 (MQ=255)
                                                                                                                                                                                                        atTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCt                                           >  2:1570157/1‑249 (MQ=255)
                                                                                                                                                                                                         ttACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTc                                          >  1:1226018/1‑249 (MQ=255)
                                                                                                                                                                                                             aaaatCAAGCCCGATTTCCGACGATTAATGCGATTTCTCTC‑TTCGTCCGAAACCGAGCTCGTCCTCAGGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGACACGCCCCGATATTCAATTTCACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTg                                      <  2:1423197/249‑1 (MQ=255)
                                                                                                                                                                                                                aaCAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGtt                                   >  1:1445293/1‑249 (MQ=255)
                                                                                                                                                                                                                aaCAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCCTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGtt                                   >  1:436080/1‑249 (MQ=255)
                                                                                                                                                                                                                 aCAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTc                                  <  2:1055202/249‑1 (MQ=255)
                                                                                                                                                                                                                      cccGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTa                             >  2:1247486/1‑249 (MQ=255)
                                                                                                                                                                                                                       ccGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTan                            >  1:318187/1‑248 (MQ=255)
                                                                                                                                                                                                                       ccGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTan                            >  2:419574/1‑248 (MQ=255)
                                                                                                                                                                                                                         ggTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGa                          >  1:488136/1‑249 (MQ=255)
                                                                                                                                                                                                                            ttCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACt                       <  1:1108783/249‑1 (MQ=255)
                                                                                                                                                                                                                              ccGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACttt                     <  2:800327/249‑1 (MQ=255)
                                                                                                                                                                                                                               cgacgaTTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTa                    >  1:636569/1‑249 (MQ=255)
                                                                                                                                                                                                                                 acgaTTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTaca                  <  1:288162/249‑1 (MQ=255)
                                                                                                                                                                                                                                    aTTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTacacac               >  2:242490/1‑249 (MQ=255)
                                                                                                                                                                                                                                         tGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGCCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAACTTCACTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGCATCGCTGACGAACTTTACACACAACCa          >  1:50846/1‑249 (MQ=255)
                                                                                                                                                                                                                                          gCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCAt         <  2:318187/249‑1 (MQ=255)
                                                                                                                                                                                                                                           cGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCATc        >  2:210448/1‑249 (MQ=255)
                                                                                                                                                                                                                                             aTTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACTCATCGAATACTAACACACTGTTATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGAGTAAGACCTTTACACACAACCATCtc      >  1:719390/1‑248 (MQ=255)
                                                                                                                                                                                                                                             aTTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCATCTg      <  2:29019/249‑1 (MQ=255)
                                                                                                                                                                                                                                              tttCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGTGCGACAGGATTTGCACCTGCGACCCCCTGCGCCCAAAGCCGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAGTACTAACCCACTCGGATCTCAGAACAAATTCAGTCAGTGACTTACTAACTCTCTGTGGTGAACCCCTGTGCGCTGGTCTCCTTGGTTCGATTAAGAGATTTACACACAACCATCTGa     >  2:228131/1‑249 (MQ=255)
                                                                                                                                                                                                                                               ttCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCATCTGaa    >  1:862465/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 ctcGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCATCTGaaan  >  2:1025501/1‑248 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
AGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑AAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGTTCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTGCGCCACGCCCCGATATTTAATTTGACCCATCGAATACTAACACACTGTGATCTCAGAACAAATTCAGTAAGTGACTTACTAAGTCTCTGTGCTGAACCCCTGTGCGCTGTGCTCCTTGGTTCGATTAAGAACTTTACACACAACCATCTGAAAA  >  CP025533/2565182‑2565671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑