Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 947,066 (T)6→7 coding (306/1755 nt) CYL77_04560 → BCCT family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP025533947,0601.T98.7% 643.6 / 0.2 155coding (300/1755 nt)CYL77_04560BCCT family transporter
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (72/81);  total (73/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 164 total aligned reads displayed.

TGCTTGAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCAT  >  CP025533/946813‑947303
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     
  cttgAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑t                                                                                                                                                                                                                                                   <  2:1552787/249‑1 (MQ=255)
       gaATCCAACTAACCTGGAAGGGAAACTGGCCAACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑ttttta                                                                                                                                                                                                                                              <  1:784530/249‑3 (MQ=255)
         aTCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑tttttatc                                                                                                                                                                                                                                            <  1:1316016/249‑5 (MQ=255)
            aaaccaaccTGGAAGTGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCgtt                                                                                                                                                                                                                                         <  2:1410274/248‑1 (MQ=255)
               cTAACCTGGAAGGGAAACTGTCCGACGCCGAAGATGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCgttgtt                                                                                                                                                                                                                                      >  1:717410/1‑249 (MQ=255)
                naaCCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTa                                                                                                                                                                                                                                     <  1:773811/248‑1 (MQ=255)
                naaCCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTa                                                                                                                                                                                                                                     <  2:1530384/248‑1 (MQ=255)
                 aaCCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTAt                                                                                                                                                                                                                                    <  2:1101985/249‑1 (MQ=255)
                         aGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCtn                                                                                                                                                                                                                            >  1:890023/1‑248 (MQ=255)
                          gggAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAg                                                                                                                                                                                                                           <  2:1531390/249‑1 (MQ=255)
                             aaaCTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTaa                                                                                                                                                                                                                        <  2:661663/249‑1 (MQ=255)
                              aaCTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTaaa                                                                                                                                                                                                                       >  1:1062056/1‑249 (MQ=255)
                                   gCCGACGGCGAAGAGGAAATTAGCCTCCAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCgg                                                                                                                                                                                                                  <  2:379848/249‑1 (MQ=255)
                                    ccGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGc                                                                                                                                                                                                                 >  1:551562/1‑249 (MQ=255)
                                          ccGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTCGTAAATTCGGCACGAtt                                                                                                                                                                                                           <  2:1468279/249‑1 (MQ=255)
                                           cGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTc                                                                                                                                                                                                          <  2:269893/249‑1 (MQ=255)
                                                   aaaTTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGc                                                                                                                                                                                                  >  1:641100/1‑249 (MQ=255)
                                                   aaaTTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGc                                                                                                                                                                                                  >  2:10699/1‑249 (MQ=255)
                                                      ctATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGAGGTGTGGGGTATCGGATTCAAAGATAGCTATACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTTGATCGTTGTTAATGCCGCTAGTAAATTCGGCACGATTTGCTTAGGCCGc                                                                                                                                                                                               >  1:691385/2‑249 (MQ=255)
                                                       tATCCTTGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCa                                                                                                                                                                                              <  1:1156391/249‑1 (MQ=255)
                                                         tCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCAtt                                                                                                                                                                                            <  1:336047/249‑1 (MQ=255)
                                                          ccTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtg                                                                                                                                                                                           <  2:798491/249‑1 (MQ=255)
                                                           cTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtga                                                                                                                                                                                          >  1:117167/1‑249 (MQ=255)
                                                           cTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtga                                                                                                                                                                                          <  2:544789/249‑1 (MQ=255)
                                                            tCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgat                                                                                                                                                                                         >  2:352125/1‑249 (MQ=255)
                                                                  gCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCa                                                                                                                                                                                   <  1:1045245/249‑1 (MQ=255)
                                                                    gAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCAcc                                                                                                                                                                                 >  2:1509270/1‑249 (MQ=255)
                                                                     aaGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCCGCCCTAGTCATTTTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCa                                                                                                                                                                                >  2:800033/1‑249 (MQ=255)
                                                                     aaGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCa                                                                                                                                                                                <  2:1140937/249‑1 (MQ=255)
                                                                     aaGACACCCAGGCCTCACTTAACGGGTCAGTCATCGTTCCAGCTCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCa                                                                                                                                                                                <  1:31521/249‑1 (MQ=255)
                                                                      aGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCag                                                                                                                                                                               <  2:1184768/249‑1 (MQ=255)
                                                                            ccAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTc                                                                                                                                                                         >  2:1057358/1‑249 (MQ=255)
                                                                                   ncACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGgt                                                                                                                                                                  <  1:795071/248‑1 (MQ=255)
                                                                                      cTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTc                                                                                                                                                               <  1:93455/249‑1 (MQ=255)
                                                                                       ntAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCa                                                                                                                                                              <  1:1570732/248‑1 (MQ=255)
                                                                                          aCTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATgg                                                                                                                                                           <  1:1149925/249‑1 (MQ=255)
                                                                                           cTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGa                                                                                                                                                          <  2:1388325/249‑1 (MQ=255)
                                                                                              gtcagtcaTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGAttt                                                                                                                                                       <  1:967033/249‑1 (MQ=255)
                                                                                                   tcaTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatg                                                                                                                                                  >  2:604010/1‑249 (MQ=255)
                                                                                                   tcaTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatg                                                                                                                                                  >  2:623436/1‑249 (MQ=255)
                                                                                                    caTCGTTCCAGCCCTATTCATTGTCCTAGCGATAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatga                                                                                                                                                 <  2:633179/249‑1 (MQ=255)
                                                                                                    caTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatga                                                                                                                                                 <  2:445639/249‑1 (MQ=255)
                                                                                                     aTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatgat                                                                                                                                                >  1:265541/1‑249 (MQ=255)
                                                                                                       cGTTCCAGCCCTAGTCATTTTCCTAGCGACAGTGGTGTGGGGGATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGt                                                                                                                                              <  1:195877/249‑1 (MQ=255)
                                                                                                        gTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TTGTTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGtt                                                                                                                                             <  1:1457265/249‑1 (MQ=255)
                                                                                                         ttCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGttt                                                                                                                                            <  2:1048040/249‑1 (MQ=255)
                                                                                                             aGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTtgctg                                                                                                                                        <  2:567596/249‑1 (MQ=255)
                                                                                                               cccTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTtgctgcn                                                                                                                                      >  1:264698/1‑248 (MQ=255)
                                                                                                                   aGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTa                                                                                                                                  >  2:327930/1‑249 (MQ=255)
                                                                                                                   aGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTa                                                                                                                                  <  1:511609/249‑1 (MQ=255)
                                                                                                                       aTTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCTCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCAGCAGAGTTTCGCACGGTGCCATAGATTTCCATGATGTTTGCTGCAGGTATggg                                                                                                                              >  2:111375/1‑249 (MQ=255)
                                                                                                                           tCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTAtt                                                                                                                          <  2:551562/249‑1 (MQ=255)
                                                                                                                           tCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTAtt                                                                                                                          <  1:152831/249‑1 (MQ=255)
                                                                                                                           tCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTAtt                                                                                                                          <  2:814255/249‑1 (MQ=255)
                                                                                                                              tAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGt                                                                                                                       >  2:1421400/1‑249 (MQ=255)
                                                                                                                                 cGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTg                                                                                                                    <  1:1101498/249‑1 (MQ=255)
                                                                                                                                      gtggtgTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGtt                                                                                                               <  1:1392188/249‑1 (MQ=255)
                                                                                                                                         gtgtGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTa                                                                                                            >  1:1013755/1‑249 (MQ=255)
                                                                                                                                                   tCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGTACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACcaca                                                                                                  <  1:1062637/249‑1 (MQ=255)
                                                                                                                                                      gATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGaa                                                                                               <  2:1012011/249‑1 (MQ=255)
                                                                                                                                                          cAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCtt                                                                                           >  1:388855/1‑249 (MQ=255)
                                                                                                                                                             aGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTaa                                                                                        <  2:516877/249‑1 (MQ=255)
                                                                                                                                                             aGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTaa                                                                                        <  1:637616/249‑1 (MQ=255)
                                                                                                                                                                   cTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCt                                                                                  <  2:101883/249‑1 (MQ=255)
                                                                                                                                                                   cTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCt                                                                                  >  2:791270/1‑249 (MQ=255)
                                                                                                                                                                    nttACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTa                                                                                 <  2:1254083/248‑1 (MQ=255)
                                                                                                                                                                       aCCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCg                                                                              >  2:1057030/1‑249 (MQ=255)
                                                                                                                                                                        ccAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGc                                                                             <  1:550903/249‑1 (MQ=255)
                                                                                                                                                                            cttacgactttCTGCGTTGTCACATGGAGTTGATCATTCCGACTTGGACCATATTATGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGATGTTAGCGCCGCTAGTAATTTCGGCAAGAATCGCTTAGGCCGCAATGATGAAGAACCAGAGTGTCGTACGGTGTCATGGATTTCCATGATGTTTGCTGCATGAATGGGTATTCGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTGCCGCAATg                                                                         <  2:1082475/240‑1 (MQ=255)
                                                                                                                                                                              ttGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGGATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGgt                                                                       >  2:865360/1‑249 (MQ=255)
                                                                                                                                                                                 cTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGAGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTACTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTa                                                                    >  2:1465909/1‑249 (MQ=255)
                                                                                                                                                                                                   cagTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAacac                                                  >  1:559353/1‑249 (MQ=255)
                                                                                                                                                                                                     gtagtTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACaa                                                >  1:1377127/1‑249 (MQ=255)
                                                                                                                                                                                                        gtTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGt                                             <  1:1463358/249‑1 (MQ=255)
                                                                                                                                                                                                         ttGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGtt                                            <  1:92027/249‑1 (MQ=255)
                                                                                                                                                                                                          tGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTCTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTg                                           >  1:858599/1‑249 (MQ=255)
                                                                                                                                                                                                             cAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCg                                        >  1:643217/1‑249 (MQ=255)
                                                                                                                                                                                                              aaTCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGt                                       <  1:429302/249‑1 (MQ=255)
                                                                                                                                                                                                               aTCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGtt                                      <  2:1191009/249‑1 (MQ=255)
                                                                                                                                                                                                                  ncGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCt                                   <  2:1100510/248‑1 (MQ=255)
                                                                                                                                                                                                                    ggCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTAt                                 <  1:143774/249‑1 (MQ=255)
                                                                                                                                                                                                                    ggCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTAt                                 >  2:1185041/1‑249 (MQ=255)
                                                                                                                                                                                                                    ggCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTAt                                 <  2:685393/249‑1 (MQ=255)
                                                                                                                                                                                                                     gCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATg                                <  2:1240777/249‑1 (MQ=255)
                                                                                                                                                                                                                         ggCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCa                            <  1:1404871/249‑1 (MQ=255)
                                                                                                                                                                                                                          gCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCAc                           <  1:1514509/249‑1 (MQ=255)
                                                                                                                                                                                                                           ccTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACg                          <  1:1509270/249‑1 (MQ=255)
                                                                                                                                                                                                                              tCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACa                       <  2:162848/249‑1 (MQ=255)
                                                                                                                                                                                                                              tCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACa                       <  2:890023/249‑1 (MQ=255)
                                                                                                                                                                                                                                  tttgtttgGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGt                   >  2:289724/1‑249 (MQ=255)
                                                                                                                                                                                                                                    tgtttgGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTc                 >  2:1329423/1‑249 (MQ=255)
                                                                                                                                                                                                                                    tgcttgGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGACTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCAATGTCCACAAACATGTTc                 >  2:1434510/4‑249 (MQ=255)
                                                                                                                                                                                                                                        tgGCACAGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACt             >  2:722707/1‑249 (MQ=255)
                                                                                                                                                                                                                                           cacaGTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGa          <  2:157891/249‑1 (MQ=255)
                                                                                                                                                                                                                                               gTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTTGCGTTGCTATGTCCACGACAATGTTCCACTGGACCtt      >  2:1339695/1‑249 (MQ=255)
                                                                                                                                                                                                                                               gTCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGAACtt      >  1:844714/1‑249 (MQ=255)
                                                                                                                                                                                                                                                tCTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTg     >  1:408863/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 nnttGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGc    <  1:180316/247‑1 (MQ=255)
                                                                                                                                                                                                                                                 cTTTGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGc    >  1:1520739/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   ttGTATTT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCAt  >  2:512670/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     
TGCTTGAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTT‑ATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCAT  >  CP025533/946813‑947303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑