BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP025533	1,611,226	(C)_7→8	noncoding (80/85 nt)	CYL77_07745 →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	1,611,219	1	.	C	89.3%	468.7 / 41.2	141	noncoding (73/85 nt)	CYL77_07745	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (2/13); new base C (72/53); total (74/67)
Fisher's exact test for biased strand distribution p-value = 1.70e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑C‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTT  >  CP025533/1610971‑1611460

aTCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGACCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGt                                                                                                                                                                                                                                                       >  2:142430/1‑249 (MQ=255)

       ggAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑c‑ccc‑c‑cc                                                                                                                                                                                                                                            <  2:695681/249‑1 (MQ=255)

       ggAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑c‑ccc‑c‑cc                                                                                                                                                                                                                                            <  2:741015/249‑1 (MQ=255)

        gAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTGCTATTAACGGCCGTGGGGGTTCAAGT‑c‑cac‑c‑ccc                                                                                                                                                                                                                                           >  1:217934/1‑248 (MQ=255)

         aaTTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑c‑ccc‑c‑cccg                                                                                                                                                                                                                                          <  2:374197/249‑3 (MQ=255)

          aTTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑C‑CCC‑C‑CCcgg                                                                                                                                                                                                                                         <  2:79063/249‑1 (MQ=255)

             cATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGca                                                                                                                                                                                                                                       >  2:1225428/1‑249 (MQ=255)

               tttGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGcaca                                                                                                                                                                                                                                     <  1:1278066/249‑1 (MQ=255)

                   tCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACtgt                                                                                                                                                                                                                                 <  1:179304/249‑1 (MQ=255)

                    cATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACtgtg                                                                                                                                                                                                                                <  1:1212923/249‑1 (MQ=255)

                      tGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGtt                                                                                                                                                                                                                              <  1:614938/249‑1 (MQ=255)

                      tGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACAATTTGtt                                                                                                                                                                                                                              >  2:1130304/1‑249 (MQ=255)

                       ggCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACtgtagtg                                                                                                                                                                                                                             >  2:691228/1‑245 (MQ=255)

                             aaCACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAg                                                                                                                                                                                                                       <  1:1404209/249‑1 (MQ=255)

                             aaCACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAg                                                                                                                                                                                                                       <  1:431371/249‑1 (MQ=255)

                              acacTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGGAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGt                                                                                                                                                                                                                      <  2:1519654/249‑1 (MQ=255)

                               cacTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTa                                                                                                                                                                                                                     >  1:573229/1‑249 (MQ=255)

                                acTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTAt                                                                                                                                                                                                                    <  1:1563876/249‑1 (MQ=255)

                                  naCCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTAttt                                                                                                                                                                                                                  <  2:574546/248‑1 (MQ=255)

                                   aCCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTa                                                                                                                                                                                                                 >  2:175270/1‑249 (MQ=255)

                                    ccGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTaa                                                                                                                                                                                                                <  1:913928/249‑1 (MQ=255)

                                        aGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGAct                                                                                                                                                                                                            <  1:1432535/249‑1 (MQ=255)

                                          cTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGActct                                                                                                                                                                                                          >  2:572113/1‑249 (MQ=255)

                                           tttATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTATTTAAATAGTATTTAacacgatg                                                                                                                                                                                                         >  2:559388/1‑245 (MQ=37)

                                            ttATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGGTTAGACAGTATTTAAGACTCTGa                                                                                                                                                                                                        >  2:434974/1‑249 (MQ=255)

                                                  ggACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTa                                                                                                                                                                                                  >  1:1346557/1‑249 (MQ=255)

                                                     cAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGGTTAGACCGTATTTAACACTCTGAACTTTAtta                                                                                                                                                                                               >  2:1329264/1‑248 (MQ=255)

                                                        tACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAg                                                                                                                                                                                            >  1:1425543/1‑249 (MQ=255)

                                                         aCTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGt                                                                                                                                                                                           <  1:1002890/249‑1 (MQ=255)

                                                          cTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTc                                                                                                                                                                                          <  2:806937/249‑1 (MQ=255)

                                                          cTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTc                                                                                                                                                                                          <  2:1101873/249‑1 (MQ=255)

                                                           ttACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACCGTATTGAGACGATCCTTAAGACACTGAACTCTATTCAAGTcc                                                                                                                                                                                         >  2:133685/1‑249 (MQ=37)

                                                             aCAAAGCTGTAAGTACTACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTccnn                                                                                                                                                                                       >  1:613198/1‑247 (MQ=255)

                                                             aCAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTccnn                                                                                                                                                                                       >  1:1154100/1‑247 (MQ=255)

                                                               aaaGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCagag                                                                                                                                                                                     <  2:976791/249‑1 (MQ=255)

                                                               aaaGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTATTTAGACCATATTTAACACTCTGAACTTTATTCCAGGCCaaag                                                                                                                                                                                     >  2:23781/1‑249 (MQ=37)

                                                                        aGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcctt                                                                                                                                                                            <  1:179158/249‑1 (MQ=255)

                                                                         gTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGGTGAGACAGCATTTAAGACTCTGAACTTTATTCAAGTCCAGTGTCTTAcctta                                                                                                                                                                           >  2:1439229/1‑249 (MQ=255)

                                                                           aCAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatg                                                                                                                                                                         >  1:310819/1‑249 (MQ=255)

                                                                              aCTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcct                                                                                                                                                                      <  2:1100691/249‑1 (MQ=255)

                                                                              aCTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcct                                                                                                                                                                      <  1:982432/249‑1 (MQ=255)

                                                                               cTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcctt                                                                                                                                                                     <  1:1249741/249‑1 (MQ=255)

                                                                                 ttGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACGCTGAATTTTATTAAAGTCCAGAGTCTTActttatgtattat                                                                                                                                                                   >  2:771681/1‑249 (MQ=255)

                                                                                     cTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGttt                                                                                                                                                               >  1:408565/1‑249 (MQ=255)

                                                                                      tttttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGAGTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGTCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑C‑CCC‑CGCCGGGCACGCTGTGTTTAGACAGTATTTAAGATTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGtttt                                                                                                                                                              >  2:1465950/1‑249 (MQ=255)

                                                                                      nttttAGCTAATGACCTGCAGATTTGCGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAATCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGTGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTGAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGtttt                                                                                                                                                              <  1:695836/248‑1 (MQ=255)

                                                                                      ntgttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGtttt                                                                                                                                                              <  2:1257151/246‑1 (MQ=255)

                                                                                        tttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGACCTTTATTCAAGTCCAGAGTCTTACCTTATGCCTGATGTTTTAc                                                                                                                                                            >  2:1364644/1‑249 (MQ=255)

                                                                                        tttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTAc                                                                                                                                                            >  1:1322771/1‑249 (MQ=255)

                                                                                         ttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGTCTATTGTTTATCTCGTGCCAAAGGCAATAAGCCCTAATCACCCAGGCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGTTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGTGTCTTACCTTATGCCTTATGTTTTACt                                                                                                                                                           <  2:853947/249‑1 (MQ=255)

                                                                                         ttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACt                                                                                                                                                           <  1:832446/249‑1 (MQ=255)

                                                                                          tAGCTAATGACCTGCAGATTTTTGTGCAGGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGCGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACGTTATTCAAGGCCAGAGTCTTACCTTATGCCTTATGTTTTACTc                                                                                                                                                          <  2:471412/249‑1 (MQ=255)

                                                                                          tAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTTAGACCATATTTTAGACGCTTACTTTTTTTCAAGTCTGATGTTTTAtcttatgtcttgttgtttaata                                                                                                                                                          >  2:166677/1‑238 (MQ=21)

                                                                                              tAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGGTTAAACCGTATTTTAGAATCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACtaatgc                                                                                                                                                      >  2:1334377/1‑244 (MQ=37)

                                                                                               aaTGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCCTACTTTTTGTCCTATTTTTTACTcacaca                                                                                                                                                     >  2:762938/1‑249 (MQ=255)

                                                                                                 tGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTTAAATAGTCTTGTAGACGCTGAACTTTATTCAAGTGCAGATTTTTGCTTTATGTCTTATGTTTTACTcatgcatt                                                                                                                                                   >  2:1237017/1‑243 (MQ=25)

                                                                                                  gACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACAccc                                                                                                                                                  <  1:1143638/249‑1 (MQ=255)

                                                                                                      tGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGGCCAAAGTCTTACCTTTTCCCTTATGTTTTACTCACACACCCAGcc                                                                                                                                              >  2:330764/1‑249 (MQ=255)

                                                                                                       gCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGccc                                                                                                                                             >  1:362601/1‑249 (MQ=255)

                                                                                                             ttGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTGATGTTTTACTCACACACCCAGCCCGGGTgc                                                                                                                                       >  2:556200/1‑248 (MQ=255)

                                                                                                                 gtTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGa                                                                                                                                   >  2:418985/1‑249 (MQ=255)

                                                                                                                  ttCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGaa                                                                                                                                  <  1:1009649/249‑1 (MQ=255)

                                                                                                                  ttCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGaa                                                                                                                                  <  1:650659/249‑1 (MQ=255)

                                                                                                                   tCAAGGAAAGATTTGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAAt                                                                                                                                 <  1:1024959/249‑1 (MQ=255)

                                                                                                                         aaaGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGa                                                                                                                           <  1:1400537/249‑1 (MQ=255)

                                                                                                                               tCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCt                                                                                                                     <  2:1179783/249‑1 (MQ=255)

                                                                                                                                    cTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTTAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAACCCGGGTAGCGGAATGGCAGACGCGCTAGCtt                                                                                                                >  2:923440/1‑249 (MQ=255)

                                                                                                                                     tATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTTAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACATTCAGCACGGGTGGCAGAATGGCAGACGCGcttgcttg                                                                                                               >  2:870803/1‑249 (MQ=255)

                                                                                                                                         gTTTATCGCTTGCCAAGGGCAAAAAGCCCTAAACACCCAGCCCGTGTTGGTGAATGGCAGACGCGCTAGCTTGAAGTGCTAGTGCAGTATGAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTGAGGCTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTAAGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGt                                                                                                           <  1:1465087/249‑1 (MQ=37)

                                                                                                                                           ttATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCCTATGTTTTACTCATACATCCAGCCCGGGGGGCGGTATGGCAGACGCGCTAGCTTGAGGTGc                                                                                                         >  2:1542239/1‑249 (MQ=255)

                                                                                                                                            tATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCt                                                                                                        >  1:973196/1‑249 (MQ=255)

                                                                                                                                             aTCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTa                                                                                                       <  1:1481160/249‑1 (MQ=255)

                                                                                                                                             aTCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGACCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCtn                                                                                                       >  1:796317/1‑248 (MQ=255)

                                                                                                                                              tctcGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACCGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAg                                                                                                      >  1:1544857/1‑249 (MQ=255)

                                                                                                                                                    gCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCt                                                                                                <  1:572113/249‑1 (MQ=255)

                                                                                                                                                      cAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACTCAGCCCTGGTGGCGGAACAGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTAt                                                                                              >  2:434622/1‑249 (MQ=255)

                                                                                                                                                            cAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACgg                                                                                        >  1:1167140/1‑249 (MQ=255)

                                                                                                                                                                aaGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGt                                                                                    >  1:119667/1‑249 (MQ=255)

                                                                                                                                                                    ccTAAACACCCCTCCGGGGTGGAGGAATGGCAGACGCACTAGCTTGGGGTGCTTGTGTCGTATTTACGGACGTGGGGGTTCAAAT‑CACCC‑C‑CCGTGCACAGTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATCCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTgggg                                                                                <  2:446896/249‑1 (MQ=37)

                                                                                                                                                                      naaaCACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGt                                                                              <  1:130678/248‑1 (MQ=255)

                                                                                                                                                                       aaaCACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGtt                                                                             <  1:973887/249‑1 (MQ=255)

                                                                                                                                                                         acacCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCa                                                                           <  1:1293666/249‑1 (MQ=255)

                                                                                                                                                                             ccAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTc                                                                       >  1:262268/1‑249 (MQ=255)

                                                                                                                                                                                                 gCAGACGCGCTAGCTTCAGGGGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAg                                                   <  1:429538/249‑1 (MQ=255)

                                                                                                                                                                                                        cgcTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATc                                            >  1:919871/1‑249 (MQ=255)

                                                                                                                                                                                                                tGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCGATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTa                                    <  1:1255335/249‑1 (MQ=255)

                                                                                                                                                                                                                  aGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACa                                  <  1:166609/249‑1 (MQ=255)

                                                                                                                                                                                                                  aGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACa                                  <  1:476221/249‑1 (MQ=255)

                                                                                                                                                                                                                   ggTTCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTCTTTAAGACTATGAATTTTATTCAAGTCTGGGGTCTTATCTTATGGATTATGGTTTACTAACACACCTAGTCCGGGGGGTGGTATGGCAGACGCGGTAGCTTGAGGTGGTAGTGTCGTATTAACTGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTTGATCActattgacaa                                 >  2:13728/1‑240 (MQ=255)

                                                                                                                                                                                                                           tgtCCTATTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTActt                         <  1:287556/249‑1 (MQ=255)

                                                                                                                                                                                                                                 aTTAACGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACTCAACCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTGGTGTCCTATTAACGTACGTCGGGGTTGAAGTCCCCCCCCGGGCCCATATTGTTTTACTCGCACTGTATAAGTTTGcttctatta                   >  2:1429146/1‑245 (MQ=37)

                                                                                                                                                                                                                                    aaCGGACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAg                >  1:1585439/1‑249 (MQ=255)

                                                                                                                                                                                                                                        gACGTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTg            >  2:1353560/1‑249 (MQ=255)

                                                                                                                                                                                                                                        gACGTGGCGGTTCAAGC‑C‑CCCTC‑CCCGTCACACTGAGTTGACTCCATATTTAAGTCCATGAACTTTATTCAAGTACAGAGTATTTCCTTATGCCTTATGTTTTCCTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTg            <  2:80878/249‑1 (MQ=25)

                                                                                                                                                                                                                                           gTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtt         >  1:165789/1‑249 (MQ=255)

                                                                                                                                                                                                                                           gTGGGGGTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAAACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCACGCCCCCCCCCGGGCACAAATTAGTTAATTCACCCTGTACAAGATTACTTATTGTACAGGGTGAtt         >  1:1305901/1‑249 (MQ=255)

                                                                                                                                                                                                                                              ggggTTCAAGT‑CACCC‑T‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTc      <  2:1285359/249‑1 (MQ=255)

                                                                                                                                                                                                                                               gggTTCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCt     <  1:1090865/249‑1 (MQ=255)

                                                                                                                                                                                                                                                  ttCAAGTCC‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGtt  >  2:343954/1‑249 (MQ=255)

                                                                                                                                                                                                                                                  ttCAAGT‑C‑CCCTC‑CCGGGCACACTGTGTTGAGACAGTATTTAAGTCTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGtt  <  1:497293/249‑1 (MQ=255)

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑C‑CCC‑C‑CCGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTT  >  CP025533/1610971‑1611460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`