Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 1,244,414 T→C V59A (GTT→GCT)  CYL77_05985 → homoserine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0255331,244,4140TC100.0% 639.8 / NA 157V59A (GTT→GCT) CYL77_05985homoserine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (70/87);  total (70/87)

Only 100 of 160 total aligned reads displayed.

GGACACATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGTTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCTG  >  CP025533/1244168‑1244658
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
 gACACATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTc                                                                                                                                                                                                                                                   <  1:176486/249‑1 (MQ=255)
   cacaTGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTg                                                                                                                                                                                                                                                 >  2:1146993/1‑249 (MQ=255)
   cacaTGTAGGGTGGCCGAAACAAAGAAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTg                                                                                                                                                                                                                                                 <  1:1031193/249‑1 (MQ=255)
      aTGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGata                                                                                                                                                                                                                                              >  1:128946/1‑249 (MQ=255)
            ggTGGCAGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCAGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGACCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCaa                                                                                                                                                                                                                                        >  2:761212/1‑249 (MQ=255)
             gTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCaaa                                                                                                                                                                                                                                       <  2:639373/249‑1 (MQ=255)
                gCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGcc                                                                                                                                                                                                                                    <  2:1242419/249‑1 (MQ=255)
                   gAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACg                                                                                                                                                                                                                                 <  1:902477/249‑1 (MQ=255)
                     aaCAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTg                                                                                                                                                                                                                               <  1:285283/249‑1 (MQ=255)
                     aaCAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTg                                                                                                                                                                                                                               <  2:926926/249‑1 (MQ=255)
                        aaaGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAg                                                                                                                                                                                                                            <  1:605016/249‑1 (MQ=255)
                        aaaGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAg                                                                                                                                                                                                                            <  2:792812/249‑1 (MQ=255)
                         aaGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAgg                                                                                                                                                                                                                           <  1:1180616/249‑1 (MQ=255)
                          aGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGc                                                                                                                                                                                                                          >  1:1063798/1‑249 (MQ=255)
                           gTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCAGTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCg                                                                                                                                                                                                                         <  1:343151/249‑1 (MQ=255)
                            tAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGt                                                                                                                                                                                                                        >  1:1355168/1‑249 (MQ=255)
                               tAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGc                                                                                                                                                                                                                     >  1:1457155/1‑249 (MQ=255)
                               tAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGc                                                                                                                                                                                                                     >  1:1301851/1‑249 (MQ=255)
                                aGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCa                                                                                                                                                                                                                    <  2:129053/249‑1 (MQ=255)
                                aGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCa                                                                                                                                                                                                                    >  2:68902/1‑249 (MQ=255)
                                 ggACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCAc                                                                                                                                                                                                                   >  1:356902/1‑249 (MQ=255)
                                  gACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCAcc                                                                                                                                                                                                                  <  2:1128958/249‑1 (MQ=255)
                                       caacGCTCGACCGCGATTATTGTTGGAGAATCAGGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTTCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAgc                                                                                                                                                                                                             <  2:592764/249‑1 (MQ=255)
                                         acGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAgctg                                                                                                                                                                                                           >  1:383481/1‑249 (MQ=255)
                                         acGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAgctg                                                                                                                                                                                                           <  2:1350527/249‑1 (MQ=255)
                                         acGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAgctg                                                                                                                                                                                                           >  1:260906/1‑249 (MQ=255)
                                          cGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAgctgc                                                                                                                                                                                                          >  1:1131112/1‑249 (MQ=255)
                                             tCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCa                                                                                                                                                                                                       >  2:626780/1‑249 (MQ=255)
                                              cGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCAc                                                                                                                                                                                                      >  1:1333636/1‑249 (MQ=255)
                                                   gcgATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAgg                                                                                                                                                                                                 <  1:538239/249‑1 (MQ=255)
                                                      attattTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACg                                                                                                                                                                                              <  2:118271/249‑1 (MQ=255)
                                                       ttattTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGc                                                                                                                                                                                             >  1:26480/1‑249 (MQ=255)
                                                           ttttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCtttt                                                                                                                                                                                         >  1:1445054/1‑249 (MQ=255)
                                                            tttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTg                                                                                                                                                                                        <  2:524478/249‑1 (MQ=255)
                                                             ttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGc                                                                                                                                                                                       <  1:375387/249‑1 (MQ=255)
                                                             ttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGc                                                                                                                                                                                       >  2:496775/1‑249 (MQ=255)
                                                              tGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCa                                                                                                                                                                                      <  2:1151448/249‑1 (MQ=255)
                                                                gagaATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGGCACGTGAAGACTGTGCACCTGAGCTGCTCTCTGAGGACGCTTCTGCACt                                                                                                                                                                                    >  1:1323658/1‑249 (MQ=255)
                                                                 agaATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTc                                                                                                                                                                                   >  1:539229/1‑249 (MQ=255)
                                                                   aaTCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCAt                                                                                                                                                                                 <  1:1216151/249‑1 (MQ=255)
                                                                     tCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCg                                                                                                                                                                               <  1:1168363/249‑1 (MQ=255)
                                                                      cATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGa                                                                                                                                                                              <  1:1543587/249‑1 (MQ=255)
                                                                        tGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAgc                                                                                                                                                                            <  1:1255881/249‑1 (MQ=255)
                                                                          aCCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAgcgc                                                                                                                                                                          >  2:801521/1‑249 (MQ=255)
                                                                              cAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCCCTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAgg                                                                                                                                                                      >  2:216739/1‑249 (MQ=255)
                                                                                 cATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATg                                                                                                                                                                   >  2:585836/1‑249 (MQ=255)
                                                                                  aTCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGt                                                                                                                                                                  >  2:1396509/1‑249 (MQ=255)
                                                                                  aTCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGt                                                                                                                                                                  <  2:33534/249‑1 (MQ=255)
                                                                                   tCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGtt                                                                                                                                                                 >  2:961625/1‑249 (MQ=255)
                                                                                    cTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTg                                                                                                                                                                >  2:1442180/1‑249 (MQ=255)
                                                                                        cccAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAt                                                                                                                                                            <  2:1063798/249‑1 (MQ=255)
                                                                                         ccAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtc                                                                                                                                                           >  1:1193023/1‑249 (MQ=255)
                                                                                         ccAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtc                                                                                                                                                           <  1:99048/249‑1 (MQ=255)
                                                                                            aGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcgtc                                                                                                                                                        <  2:204468/249‑1 (MQ=255)
                                                                                             gCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcgtcg                                                                                                                                                       <  2:1452067/249‑1 (MQ=255)
                                                                                             gCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcgtcg                                                                                                                                                       <  2:1044300/249‑1 (MQ=255)
                                                                                             gCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcgtcg                                                                                                                                                       <  1:1197187/249‑1 (MQ=255)
                                                                                                 tAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGGACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACCTCGTCGTTgn                                                                                                                                                   >  1:1023851/1‑248 (MQ=255)
                                                                                                 tAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGa                                                                                                                                                   <  1:226588/249‑1 (MQ=255)
                                                                                                   aCCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAgg                                                                                                                                                 <  2:565023/249‑1 (MQ=255)
                                                                                                     cccGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGtt                                                                                                                                               >  1:1544422/1‑249 (MQ=255)
                                                                                                        ggCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATc                                                                                                                                            <  2:1501139/249‑1 (MQ=255)
                                                                                                        ggCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATc                                                                                                                                            <  2:87342/249‑1 (MQ=255)
                                                                                                          cAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATcgg                                                                                                                                          >  2:858800/1‑249 (MQ=255)
                                                                                                                  ccGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTg                                                                                                                                  >  1:596708/1‑249 (MQ=255)
                                                                                                                   cGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTgn                                                                                                                                 >  2:1160792/1‑248 (MQ=255)
                                                                                                                               cGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTgn                                                                                                                     >  1:430242/1‑248 (MQ=255)
                                                                                                                                ggAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAg                                                                                                                    <  2:835441/249‑1 (MQ=255)
                                                                                                                                   aTTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGtn                                                                                                                 >  1:864880/1‑248 (MQ=255)
                                                                                                                                         cTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTtctc                                                                                                           <  1:1347257/249‑1 (MQ=255)
                                                                                                                                              aGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGc                                                                                                      >  2:191024/1‑249 (MQ=255)
                                                                                                                                               ggATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGct                                                                                                     <  1:97832/249‑1 (MQ=255)
                                                                                                                                                gATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGctc                                                                                                    >  1:168893/1‑249 (MQ=255)
                                                                                                                                                 aTTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGctct                                                                                                   <  1:662039/249‑1 (MQ=255)
                                                                                                                                                      gAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAgg                                                                                              <  2:1261556/249‑1 (MQ=255)
                                                                                                                                                      gAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAgg                                                                                              <  1:927918/249‑1 (MQ=255)
                                                                                                                                                       aaCAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGc                                                                                             >  2:1313289/1‑249 (MQ=255)
                                                                                                                                                          aGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCgg                                                                                          >  2:1084371/1‑249 (MQ=255)
                                                                                                                                                           gTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTCGTTCTCGCAGCTCTGAAGGCCGGc                                                                                         >  2:431440/1‑249 (MQ=255)
                                                                                                                                                            tCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCa                                                                                        <  1:1210263/249‑1 (MQ=255)
                                                                                                                                                                      ggTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCtgttgt                                                                              >  1:661125/1‑249 (MQ=255)
                                                                                                                                                                          aTGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTAcc                                                                          <  1:998597/249‑1 (MQ=255)
                                                                                                                                                                           tGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCg                                                                         <  2:34145/249‑1 (MQ=255)
                                                                                                                                                                           tGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGACATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCg                                                                         >  2:1473046/1‑249 (MQ=255)
                                                                                                                                                                                    tgaCCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAgg                                                                >  1:593003/1‑249 (MQ=255)
                                                                                                                                                                                      aCCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGct                                                              <  1:45037/249‑1 (MQ=255)
                                                                                                                                                                                          aGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCttg                                                          <  1:72405/249‑1 (MQ=255)
                                                                                                                                                                                                   atgaACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTc                                                 <  2:376841/249‑1 (MQ=255)
                                                                                                                                                                                                   atgaACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTc                                                 <  2:1301851/249‑1 (MQ=255)
                                                                                                                                                                                                     gaACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGACGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCAc                                               >  1:528710/1‑249 (MQ=255)
                                                                                                                                                                                                      aaCTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCAct                                              >  2:401886/1‑249 (MQ=255)
                                                                                                                                                                                                        cTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCActct                                            >  1:487980/1‑249 (MQ=255)
                                                                                                                                                                                                          tGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCACAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTctgc                                          <  1:1009833/249‑1 (MQ=255)
                                                                                                                                                                                                           gcgcACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTctgct                                         >  2:1246091/1‑249 (MQ=255)
                                                                                                                                                                                                                 cGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCtt                                   <  2:1333636/249‑1 (MQ=255)
                                                                                                                                                                                                                        gtggCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATg                            <  1:809341/249‑1 (MQ=255)
                                                                                                                                                                                                                                     ggTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGTCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGc               <  2:974672/249‑1 (MQ=255)
                                                                                                                                                                                                                                          gTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACg          >  2:1123970/1‑249 (MQ=255)
                                                                                                                                                                                                                                           tGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAACTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGt         >  2:750356/1‑249 (MQ=255)
                                                                                                                                                                                                                                                  gctgctTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCTg  <  1:284388/249‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
GGACACATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGTTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCTG  >  CP025533/1244168‑1244658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑