Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 2,419,925 A→G Q194Q (CAA→CAG)  CYL77_11475 → HNH endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0255332,419,9250AG81.9% 200.1 / 74.9 150Q194Q (CAA→CAG) CYL77_11475HNH endonuclease
Reads supporting (aligned to +/- strand):  ref base A (19/8);  new base G (74/48);  total (94/56)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 150 total aligned reads displayed.

CAACGGCGGTAATGGTGGCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTCGTGGATCTA  >  CP025533/2419679‑2420156
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
cAACGGCGGTAATGGTGGCAATCCCGGTGGCGGTCCTGATGGTGGCGACTAGGGTGATGACTACTCCGGCGATGATGATCCCGACCCCTTTTTTTTCAAGGCTGAAGACGGCATACGAGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGc                                                                                                                                                                                                                                        <  1:175826/249‑1 (MQ=11)
  aCGGCGGTAATGGTGGCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGa                                                                                                                                                                                                                                      >  2:218022/1‑249 (MQ=21)
      cggTAATGGTGGCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTg                                                                                                                                                                                                                                  >  2:1539782/1‑249 (MQ=21)
       ggTAATGGTGGCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTgg                                                                                                                                                                                                                                 >  2:1301963/1‑249 (MQ=21)
        gTAATGGTGGCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGgc                                                                                                                                                                                                                                <  1:864688/249‑1 (MQ=21)
             ggtggCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCAcc                                                                                                                                                                                                                           >  1:183355/1‑249 (MQ=25)
               tggCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCAcccc                                                                                                                                                                                                                         >  2:1334488/1‑249 (MQ=21)
                   aaTCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCagan                                                                                                                                                                                                                     >  2:962811/1‑248 (MQ=21)
                          aTGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTcc                                                                                                                                                                                                              >  1:437123/1‑249 (MQ=18)
                           tGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCg                                                                                                                                                                                                             <  1:1425971/249‑1 (MQ=18)
                                 tCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTg                                                                                                                                                                                                       <  2:286269/249‑1 (MQ=11)
                                       tggtggCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑Cg                                                                                                                                                                                                <  1:510657/249‑1 (MQ=14)
                                       tggtggCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑Cg                                                                                                                                                                                                <  1:1355031/249‑1 (MQ=14)
                                           ggCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGccac                                                                                                                                                                                            <  1:144092/249‑1 (MQ=14)
                                              gACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAg                                                                                                                                                                                         >  1:709798/1‑249 (MQ=17)
                                               aCTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAgg                                                                                                                                                                                        >  1:567593/1‑249 (MQ=14)
                                                 ncGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTc                                                                                                                                                                                      <  1:521546/248‑1 (MQ=14)
                                                      tgatgaCGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTcaccac                                                                                                                                                                                 >  1:1356330/1‑249 (MQ=11)
                                                       gatgaCGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCaccacc                                                                                                                                                                                >  2:769294/1‑249 (MQ=17)
                                                               cTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACaa                                                                                                                                                                        >  1:277680/1‑249 (MQ=14)
                                                                  cGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACaaaaa                                                                                                                                                                     <  1:22690/249‑1 (MQ=14)
                                                                         gatgaCCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAAcaacccc                                                                                                                                                              >  2:1288637/1‑249 (MQ=14)
                                                                          atgcCCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAAcaacccca                                                                                                                                                             <  2:914093/249‑1 (MQ=11)
                                                                          atgaCCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAAcaacccca                                                                                                                                                             <  1:1252867/249‑1 (MQ=11)
                                                                            gaccccgaccccGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAAcaaccccaac                                                                                                                                                           <  1:364328/249‑1 (MQ=14)
                                                                              ccccgaccccgaACCGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAAcaaccccaaccc                                                                                                                                                         >  1:1538133/1‑249 (MQ=11)
                                                                                  gaccccgaACCGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATc                                                                                                                                                     >  2:1227652/1‑249 (MQ=11)
                                                                                   accccgaACCGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCa                                                                                                                                                    >  2:722265/1‑249 (MQ=255)
                                                                                    ccccgaACCGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCAc                                                                                                                                                   >  2:25514/1‑249 (MQ=11)
                                                                                        gaACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCGCCACCGCGAACAAAAACAACCCCAACCCCATCACCGcc                                                                                                                                               >  2:1462166/1‑249 (MQ=14)
                                                                                           ccGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATg                                                                                                                                            >  2:482825/1‑249 (MQ=11)
                                                                                            cGGACAAGCCTGAAGACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAAAAGACCTCCGCACGT‑GCTACCGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCACGa                                                                                                                                           >  1:848061/1‑249 (MQ=11)
                                                                                            cGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGa                                                                                                                                           >  2:480137/1‑249 (MQ=14)
                                                                                             ggACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGaa                                                                                                                                          >  2:41684/1‑249 (MQ=14)
                                                                                               aCAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGaaaa                                                                                                                                        <  2:535735/249‑1 (MQ=14)
                                                                                                 aaGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGACAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCCCCGCCATGAAAAgg                                                                                                                                      >  1:1199588/1‑249 (MQ=14)
                                                                                                 aaGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAgg                                                                                                                                      >  2:825615/1‑249 (MQ=14)
                                                                                                   gCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCGCCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGgcg                                                                                                                                    <  1:1072569/249‑1 (MQ=14)
                                                                                                   gCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGgcg                                                                                                                                    >  2:1072881/1‑249 (MQ=11)
                                                                                                      ngAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTa                                                                                                                                 <  1:583464/248‑1 (MQ=14)
                                                                                                       gaagaaGGCAAACCTGATAGTGATAAGCCCCGTATGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTAc                                                                                                                                <  2:689007/249‑1 (MQ=14)
                                                                                                       gAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTAc                                                                                                                                <  1:1255722/249‑1 (MQ=14)
                                                                                                         aGACGGCATACGAGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACct                                                                                                                              <  1:806428/249‑1 (MQ=9)
                                                                                                         aGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACct                                                                                                                              >  1:291139/1‑249 (MQ=14)
                                                                                                          gACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctc                                                                                                                             <  2:565437/249‑1 (MQ=255)
                                                                                                          gACGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctc                                                                                                                             >  2:1169467/1‑249 (MQ=11)
                                                                                                           aCGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctcg                                                                                                                            >  2:1310489/1‑248 (MQ=11)
                                                                                                           aCGGCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctcg                                                                                                                            >  1:1189123/1‑248 (MQ=11)
                                                                                                           aCGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctct                                                                                                                            <  1:70249/249‑1 (MQ=14)
                                                                                                             ggCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCa                                                                                                                          <  1:669018/249‑1 (MQ=14)
                                                                                                              gCAAACCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctcgctg                                                                                                                         >  2:938755/1‑245 (MQ=11)
                                                                                                               cAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGt                                                                                                                        <  1:760738/249‑1 (MQ=14)
                                                                                                                aaaCCTGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACctcgctgta                                                                                                                       <  1:1042298/249‑7 (MQ=11)
                                                                                                                  aCCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAgg                                                                                                                     >  2:1484093/1‑249 (MQ=14)
                                                                                                                    cTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTa                                                                                                                   >  1:1139608/1‑249 (MQ=14)
                                                                                                                     tGATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCACGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCGCTGTAGGTaa                                                                                                                  >  1:919668/1‑249 (MQ=11)
                                                                                                                     ngATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTaa                                                                                                                  <  2:1140003/248‑1 (MQ=17)
                                                                                                                     ngATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTaa                                                                                                                  <  2:277680/248‑1 (MQ=14)
                                                                                                                      gATGGTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCGCTGTAGGTaaa                                                                                                                 <  2:1328374/249‑1 (MQ=11)
                                                                                                                      gATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTaaa                                                                                                                 >  2:1386752/1‑249 (MQ=14)
                                                                                                                       atggTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCGCTGTAGGTAAAc                                                                                                                <  1:233522/246‑1 (MQ=11)
                                                                                                                       aTAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAAc                                                                                                                >  2:1036224/1‑249 (MQ=17)
                                                                                                                          gTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCGCTGTAGGTAAACCCg                                                                                                             >  2:1014414/1‑249 (MQ=11)
                                                                                                                          gTGATAAGCCTCGTGGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAGCTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCACGTCACCACCGCCAATAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCGCTGTAGGTAAACCCg                                                                                                             >  1:46634/1‑249 (MQ=11)
                                                                                                                          gTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCg                                                                                                             <  2:254166/249‑1 (MQ=11)
                                                                                                                            gATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGAt                                                                                                           <  1:897355/249‑1 (MQ=14)
                                                                                                                               aaGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGcc                                                                                                        >  2:62782/1‑249 (MQ=14)
                                                                                                                                  ccccGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGac                                                                                                     >  2:457582/1‑249 (MQ=14)
                                                                                                                                       tAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACAt                                                                                                <  1:1327741/249‑1 (MQ=14)
                                                                                                                                         ggCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCCCGTGGCTA‑CACCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCGTGAAAGGGCGCTACCTCGCAGCAGGCAAACCCAACGCCGACAACATgg                                                                                              >  2:1157868/1‑249 (MQ=11)
                                                                                                                                           ccACGTATCAGCGCGGAAAAACACGCCATCATCACCGCCGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGCACTACGCACACAAGCCCTGAGTCAGGCGATCTGGCGCAACCCAGAAGACCTCCGCACGTGGCTT‑CGCCAACAGGTCACCACCGCGAACAAAACAAACCCCAACCCCATCACCGCACTGAAAAGGCGCCACCTCTCAGTAGGTAAACCCGCTGCCGCAAACCTgggg                                                                                            >  2:96182/1‑247 (MQ=11)
                                                                                                                                            cACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTcc                                                                                           >  1:728608/1‑249 (MQ=14)
                                                                                                                                             aCGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCg                                                                                          <  1:898782/249‑1 (MQ=14)
                                                                                                                                              cGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGc                                                                                         >  2:1174863/1‑249 (MQ=14)
                                                                                                                                                gATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCAt                                                                                       <  2:310125/249‑1 (MQ=14)
                                                                                                                                                   cAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAg                                                                                    <  1:1377206/249‑1 (MQ=11)
                                                                                                                                                         ggAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCt                                                                              >  1:102560/1‑249 (MQ=17)
                                                                                                                                                                cacGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGccc                                                                       >  1:1408042/1‑249 (MQ=14)
                                                                                                                                                                 acGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCg                                                                      <  1:1087945/249‑1 (MQ=11)
                                                                                                                                                                      atcatcACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGcc                                                                 >  1:452377/1‑249 (MQ=14)
                                                                                                                                                                           caCCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCgc                                                            >  2:1361934/1‑249 (MQ=14)
                                                                                                                                                                              cgacgaACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATAGTCCACATCAGCGGCCTCGTGCCCGCAGCCACCgcagc                                                         >  2:1140588/1‑249 (MQ=14)
                                                                                                                                                                                acgaACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCAc                                                       >  1:840203/1‑249 (MQ=14)
                                                                                                                                                                                 cgaACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACt                                                      >  1:919118/1‑249 (MQ=14)
                                                                                                                                                                                 cgaACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACt                                                      >  2:634229/1‑249 (MQ=14)
                                                                                                                                                                                  gaACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGCCCGCGTCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTg                                                     >  2:1040110/1‑249 (MQ=14)
                                                                                                                                                                                    aCTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGAt                                                   >  1:328951/1‑249 (MQ=14)
                                                                                                                                                                                     cTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATc                                                  <  1:661694/249‑1 (MQ=14)
                                                                                                                                                                                     cTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATc                                                  >  2:1057184/1‑249 (MQ=14)
                                                                                                                                                                                       cgcccgccTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCAc                                                >  1:439421/1‑249 (MQ=14)
                                                                                                                                                                                                 aaCCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAAcacc                                      >  2:1486831/1‑249 (MQ=14)
                                                                                                                                                                                                 aaCCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAAcacc                                      >  2:864643/1‑249 (MQ=14)
                                                                                                                                                                                                          aCCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTa                             <  1:1241648/249‑1 (MQ=14)
                                                                                                                                                                                                                       ccGAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCa                >  1:1441059/1‑249 (MQ=17)
                                                                                                                                                                                                                         gAGGAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAAc              >  2:1087242/1‑249 (MQ=17)
                                                                                                                                                                                                                            gAACTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTc           <  1:1572529/249‑1 (MQ=18)
                                                                                                                                                                                                                              aCTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTCGt         >  1:797653/1‑249 (MQ=17)
                                                                                                                                                                                                                               cTACGCACCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTCGTg        >  2:1290980/1‑249 (MQ=17)
                                                                                                                                                                                                                                tACGCACCCAAGCCCTGTGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTCGTgg       >  1:867379/1‑249 (MQ=17)
                                                                                                                                                                                                                                     aCCCAAGCCCTGAGTCAGGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTCGTGGATCTa  >  2:1406531/1‑249 (MQ=11)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
CAACGGCGGTAATGGTGGCAATCCCGATGGCGGTCCTGATGGTGGCGACTCGGGTGATGACGACTCCGGCGATGATGACCCCGACCCCGAACCGGACAAGCCTGAAGACGGCAAACCTGATAGTGATAAGCCCCGTAGGCCACGGATCAGCGCGGAAAAACACGCCATCATCACCGACGAACTCGCCCGCCTCAACCCGAATACCACACCCAGCGCCGAGGAACTACGCACCCAAGCCCTGAGTCAAGCGATCTGGCGCACCCCAGAAGACCTCCGCACGTGGCTA‑CGCCACCAGGTCACCACCGCGAACAAAAACAACCCCAACCCCATCACCGCCATGAAAAGGCGCTACCTCTCAGTAGGTAAACCCGATGCCGACAACATGGTCCGCATCAGCGGCCTCGTGCCCGCAGCCACCGCAGCACTGATCACCGCGAACACCGCACCGTTAACCAAACGTGGCAACCTCGTGGATCTA  >  CP025533/2419679‑2420156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑