Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 947,066 (T)6→7 coding (306/1755 nt) CYL77_04560 → BCCT family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP025533947,0601.T99.1% 454.4 / ‑3.9 110coding (300/1755 nt)CYL77_04560BCCT family transporter
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (58/51);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 116 total aligned reads displayed.

TTGCTTGAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCAT  >  CP025533/946812‑947303
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
ntgcttgAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTa                                                                                                                                                                                                                                                       <  2:640344/248‑1 (MQ=255)
   cttgAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑t                                                                                                                                                                                                                                                  >  2:846069/1‑249 (MQ=255)
    ttgAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGTGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑tt                                                                                                                                                                                                                                                 >  1:131115/1‑249 (MQ=255)
       agaATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTGCATTTTGGTTGGCACAGTATTTGTA‑tt‑ttttt                                                                                                                                                                                                                                              >  2:265699/1‑248 (MQ=255)
         aaTCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑tttttat                                                                                                                                                                                                                                            <  2:732669/249‑4 (MQ=255)
            ccAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTACCTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCgt                                                                                                                                                                                                                                          <  1:168970/249‑1 (MQ=255)
                         aaGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCt                                                                                                                                                                                                                             >  1:667997/1‑249 (MQ=255)
                          aGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTa                                                                                                                                                                                                                            <  1:257700/249‑1 (MQ=255)
                              aaaCTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTaa                                                                                                                                                                                                                        >  2:380990/1‑249 (MQ=255)
                               aaCTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTAAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGCTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTAGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACACTCTTTGTATTT‑TTTTATCGTTGTTATCGCAGCTAGTaag                                                                                                                                                                                                                       >  2:507388/1‑248 (MQ=255)
                                aCTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAAt                                                                                                                                                                                                                      <  1:817245/249‑1 (MQ=255)
                                  tGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTc                                                                                                                                                                                                                    >  1:811363/1‑249 (MQ=255)
                                     ccGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGc                                                                                                                                                                                                                 <  1:845146/249‑1 (MQ=255)
                                      cGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCa                                                                                                                                                                                                                <  2:804979/249‑1 (MQ=255)
                                        aCGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACg                                                                                                                                                                                                              <  1:94143/249‑1 (MQ=255)
                                        aCGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACg                                                                                                                                                                                                              >  1:705441/1‑249 (MQ=255)
                                         cGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGa                                                                                                                                                                                                             >  2:222671/1‑249 (MQ=255)
                                                 agGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTa                                                                                                                                                                                                     <  2:812973/249‑1 (MQ=255)
                                                    aaaTTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGc                                                                                                                                                                                                  >  1:831317/1‑249 (MQ=255)
                                                           ccTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtg                                                                                                                                                                                           >  1:1254218/1‑249 (MQ=255)
                                                              nnaaGGCGAAGACACCCAGGCCTCACTTAACGCGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgatg                                                                                                                                                                                        <  2:98026/247‑1 (MQ=255)
                                                              cGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgatg                                                                                                                                                                                        >  1:626843/1‑249 (MQ=255)
                                                              cGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgatg                                                                                                                                                                                        <  2:823663/249‑1 (MQ=255)
                                                               gAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGGTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgatga                                                                                                                                                                                       >  2:1198824/1‑249 (MQ=255)
                                                                aaGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgatgan                                                                                                                                                                                      >  1:150952/1‑248 (MQ=255)
                                                                 aGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAg                                                                                                                                                                                     <  2:526209/249‑1 (MQ=255)
                                                                  ggCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGc                                                                                                                                                                                    <  1:259285/249‑1 (MQ=255)
                                                                   gCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCa                                                                                                                                                                                   <  2:881370/249‑1 (MQ=255)
                                                                    cGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCAc                                                                                                                                                                                  <  1:897121/249‑1 (MQ=255)
                                                                        gACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCagn                                                                                                                                                                              >  2:802112/1‑248 (MQ=255)
                                                                          cacCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGt                                                                                                                                                                            >  2:1249317/1‑249 (MQ=255)
                                                                          cacCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCAAGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGt                                                                                                                                                                            <  2:670105/249‑1 (MQ=255)
                                                                              cAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCg                                                                                                                                                                        <  1:805848/249‑1 (MQ=255)
                                                                                 gCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCAc                                                                                                                                                                     <  1:821980/249‑1 (MQ=255)
                                                                                   cTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACgg                                                                                                                                                                   <  1:236607/249‑1 (MQ=255)
                                                                                     cACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGgtg                                                                                                                                                                 >  2:1264159/1‑249 (MQ=255)
                                                                                     cACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTCATGAAGCACCAGAGTTTCGCACGgtg                                                                                                                                                                 >  2:157439/1‑249 (MQ=255)
                                                                                     cACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGATTAGGCCGCATAGATGAAGCACCAGAGCTTCGCACGgtg                                                                                                                                                                 >  2:157440/1‑249 (MQ=255)
                                                                                        ttAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCa                                                                                                                                                              >  1:883556/1‑249 (MQ=255)
                                                                                         tAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCAt                                                                                                                                                             <  1:886636/249‑1 (MQ=255)
                                                                                          aaCTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATg                                                                                                                                                            >  2:632931/1‑249 (MQ=255)
                                                                                             nggTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGAt                                                                                                                                                         <  1:235824/248‑1 (MQ=255)
                                                                                              ggTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGAtt                                                                                                                                                        <  2:235898/249‑1 (MQ=255)
                                                                                               gtcagtcaTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGAttt                                                                                                                                                       <  1:594011/249‑1 (MQ=255)
                                                                                                   gtcaTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCat                                                                                                                                                   <  2:493797/249‑1 (MQ=255)
                                                                                                     caTCGTTCCAGCCCAAGTCATTGTCCTAGCGACAGTGGTGTGTGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatga                                                                                                                                                 <  1:687595/249‑1 (MQ=255)
                                                                                                       tCGTTCCATCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTAGCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatgatg                                                                                                                                               <  1:507526/249‑1 (MQ=255)
                                                                                                             cAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTtgct                                                                                                                                         >  1:221926/1‑249 (MQ=255)
                                                                                                              aGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTtgctg                                                                                                                                        <  2:469080/249‑1 (MQ=255)
                                                                                                              aGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTtgctg                                                                                                                                        >  2:1230539/1‑249 (MQ=255)
                                                                                                                 ccTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAg                                                                                                                                     <  1:462534/249‑1 (MQ=255)
                                                                                                                        aTTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATggg                                                                                                                              <  1:308558/249‑1 (MQ=255)
                                                                                                                            tCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTAtt                                                                                                                          <  2:46327/249‑1 (MQ=255)
                                                                                                                                aGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGtt                                                                                                                      >  1:590818/1‑249 (MQ=255)
                                                                                                                                 gCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGttt                                                                                                                     <  1:320040/249‑1 (MQ=255)
                                                                                                                                 gCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGttt                                                                                                                     <  2:953689/249‑1 (MQ=255)
                                                                                                                                 gCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGttt                                                                                                                     >  1:360544/1‑249 (MQ=255)
                                                                                                                                     cAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATg                                                                                                                 >  1:148771/1‑249 (MQ=255)
                                                                                                                                      aGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGt                                                                                                                >  1:744204/1‑249 (MQ=255)
                                                                                                                                       gtggtgTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGtt                                                                                                               <  1:412628/249‑1 (MQ=255)
                                                                                                                                              ggggTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGTCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGa                                                                                                        >  1:591270/1‑249 (MQ=255)
                                                                                                                                                    tCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACcaca                                                                                                  <  2:173334/249‑1 (MQ=255)
                                                                                                                                                     cGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAg                                                                                                 <  2:1226681/249‑1 (MQ=255)
                                                                                                                                                      ggATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGa                                                                                                >  1:275958/1‑249 (MQ=255)
                                                                                                                                                          tCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCt                                                                                            >  1:1189441/1‑249 (MQ=255)
                                                                                                                                                          tCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCt                                                                                            <  1:558274/249‑1 (MQ=255)
                                                                                                                                                           cAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCtt                                                                                           >  1:1121866/1‑249 (MQ=255)
                                                                                                                                                               gATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAAc                                                                                       <  2:53717/249‑1 (MQ=255)
                                                                                                                                                                    cTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCt                                                                                  <  1:248276/249‑1 (MQ=255)
                                                                                                                                                                      ttACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTAc                                                                                <  2:861461/249‑1 (MQ=255)
                                                                                                                                                                      ttACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTAc                                                                                >  1:285351/1‑249 (MQ=255)
                                                                                                                                                                      ttACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTAc                                                                                <  1:765497/249‑1 (MQ=255)
                                                                                                                                                                       tACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTAcc                                                                               >  1:671190/1‑249 (MQ=255)
                                                                                                                                                                         ccAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGc                                                                             >  1:728770/1‑249 (MQ=255)
                                                                                                                                                                              tttGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATgg                                                                        >  2:429693/1‑249 (MQ=255)
                                                                                                                                                                               ttGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAATCTTCTACCGCAATGgt                                                                       >  2:1193953/1‑249 (MQ=255)
                                                                                                                                                                               ttGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGgt                                                                       >  1:869866/1‑249 (MQ=255)
                                                                                                                                                                               ttGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAACTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGgt                                                                       >  2:587130/1‑249 (MQ=255)
                                                                                                                                                                                tGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGgtg                                                                      >  1:790850/1‑249 (MQ=255)
                                                                                                                                                                                   tAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTAc                                                                   >  1:659019/1‑249 (MQ=255)
                                                                                                                                                                                     gtttttcgatGTCAGCAGTAGTTGACCAACCGAGCATGGCCTGTATTTTGTTTAGCACAGAGTTTGTA‑TTGTACGCTCGTTGTTATCGCCGCTAGTAAACTCGACACGATTCGCTTATGCCGCATTGATGACTCACCAGAGTTTCGCACGGTGTCATGGATAACCATGATGTTTGCCGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTATACCt                                                                 <  2:90190/240‑1 (MQ=255)
                                                                                                                                                                                     gTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCt                                                                 >  2:790221/1‑249 (MQ=255)
                                                                                                                                                                                        nngCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTAATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGa                                                              <  2:1108908/247‑1 (MQ=255)
                                                                                                                                                                                               gTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACatgatg                                                       >  2:199307/1‑249 (MQ=255)
                                                                                                                                                                                                  agcagTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAac                                                    >  2:386454/1‑249 (MQ=255)
                                                                                                                                                                                                             aCAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGc                                         <  1:832154/249‑1 (MQ=255)
                                                                                                                                                                                                              cAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCg                                        <  2:606019/249‑1 (MQ=255)
                                                                                                                                                                                                              cAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCg                                        >  1:743956/1‑249 (MQ=255)
                                                                                                                                                                                                                 tctcGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTg                                     >  1:406062/1‑249 (MQ=255)
                                                                                                                                                                                                                     ggCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTAt                                 <  1:973044/249‑1 (MQ=255)
                                                                                                                                                                                                                                  ttttGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATg                    >  1:975084/1‑249 (MQ=255)
                                                                                                                                                                                                                                   tttgtttgGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGt                   <  1:632931/249‑1 (MQ=255)
                                                                                                                                                                                                                                      gtttgGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTcc                <  1:1239305/249‑1 (MQ=255)
                                                                                                                                                                                                                                       tttgGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCa               >  2:632012/1‑249 (MQ=255)
                                                                                                                                                                                                                                         tgGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACt             <  1:222671/249‑1 (MQ=255)
                                                                                                                                                                                                                                          ggCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTg            <  1:713929/249‑1 (MQ=255)
                                                                                                                                                                                                                                            cacaGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGa          >  1:590173/1‑249 (MQ=255)
                                                                                                                                                                                                                                               aGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCt       <  2:148771/249‑1 (MQ=255)
                                                                                                                                                                                                                                                   tttGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCa   >  2:385331/1‑249 (MQ=255)
                                                                                                                                                                                                                                                    ttGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCAt  >  1:489650/1‑249 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
TTGCTTGAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGCAT  >  CP025533/946812‑947303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑