BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP025533	1,611,226	(C)_7→8	noncoding (80/85 nt)	CYL77_07745 →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	1,611,219	1	.	C	97.0%	368.0 / 1.3	103	noncoding (73/85 nt)	CYL77_07745	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (59/39); total (59/44)
Fisher's exact test for biased strand distribution p-value = 6.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 109 total aligned reads displayed.

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑CCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTTT  >  CP025533/1610971‑1611461

aTCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGt                                                                                                                                                                                                                                                        >  1:751070/1‑249 (MQ=255)

aTCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGt                                                                                                                                                                                                                                                        <  1:176571/249‑1 (MQ=255)

 tCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑c                                                                                                                                                                                                                                                      <  1:278651/249‑1 (MQ=255)

     ggggAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑cccc‑c                                                                                                                                                                                                                                                 >  1:90722/1‑249 (MQ=255)

         aaTTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGGGGGGGTACAAGT‑cccc‑c‑c‑ccg                                                                                                                                                                                                                                           >  1:930916/1‑247 (MQ=255)

                  gTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACtg                                                                                                                                                                                                                                   >  1:661224/1‑249 (MQ=255)

                      tGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGtt                                                                                                                                                                                                                               >  2:244259/1‑249 (MQ=255)

                         cGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTgag                                                                                                                                                                                                                            <  2:1098077/249‑1 (MQ=255)

                          gAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCGCACtgtggttaaa                                                                                                                                                                                                                           >  2:745383/1‑243 (MQ=255)

                          gAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTTTGTTgaaa                                                                                                                                                                                                                           >  2:835422/1‑247 (MQ=255)

                           aaaaCACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGAc                                                                                                                                                                                                                          >  2:627199/1‑249 (MQ=255)

                             aaCACTACCGCAGCTTTATACGGATAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACTTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAg                                                                                                                                                                                                                        <  2:213847/249‑1 (MQ=255)

                               cacTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTa                                                                                                                                                                                                                      <  2:804927/249‑1 (MQ=255)

                                  naCCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGGCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTAttt                                                                                                                                                                                                                   <  2:1189391/248‑1 (MQ=255)

                                    ccGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACAAGGTGCTGAGAccatccttta                                                                                                                                                                                                                 >  2:164877/1‑240 (MQ=255)

                                      gCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGa                                                                                                                                                                                                               <  2:209947/249‑1 (MQ=255)

                                       cAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAAACCGTCTTTAacac                                                                                                                                                                                                              >  2:259280/1‑249 (MQ=255)

                                       cAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGAc                                                                                                                                                                                                              >  1:1137010/1‑249 (MQ=255)

                                        aGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTTTGGTTAGATAGTCTTTAAGAct                                                                                                                                                                                                             >  2:738801/1‑249 (MQ=255)

                                        aGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAAACCGTCTTTAAGAct                                                                                                                                                                                                             >  2:115984/1‑249 (MQ=255)

                                        aGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGAct                                                                                                                                                                                                             <  1:406869/249‑1 (MQ=255)

                                          cTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAGACAGTATTTAAGActct                                                                                                                                                                                                           >  2:445077/1‑249 (MQ=255)

                                                 cGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACttt                                                                                                                                                                                                    >  1:904759/1‑249 (MQ=255)

                                                        tACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAg                                                                                                                                                                                             <  1:1240467/249‑1 (MQ=255)

                                                        tACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAg                                                                                                                                                                                             <  2:939765/249‑1 (MQ=255)

                                                        tACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGGTTAGACAGTATTTAAGACTCTTACCTTTAGTAAAg                                                                                                                                                                                             >  2:1204046/1‑249 (MQ=255)

                                                          cTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTc                                                                                                                                                                                           <  2:846925/249‑1 (MQ=255)

                                                           ntACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTcc                                                                                                                                                                                          <  1:402089/248‑1 (MQ=255)

                                                            tACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGGTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTccn                                                                                                                                                                                         >  1:663457/1‑248 (MQ=255)

                                                               aaaGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCagag                                                                                                                                                                                      <  1:244259/249‑1 (MQ=255)

                                                                 aGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTTTGTTGAGACAGTACTTAAGACTCTGAACTTTATTCAAGTCCAGAGTc                                                                                                                                                                                    >  1:882263/1‑249 (MQ=255)

                                                                      tAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcc                                                                                                                                                                               <  2:226936/249‑1 (MQ=255)

                                                                        aGTACAATTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcctt                                                                                                                                                                             <  1:966131/249‑1 (MQ=255)

                                                                          tACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTTTGTTTAGATCATATTTTAGACTCTGAACTTTATTCCAGGGCGAAGTGTTAccttat                                                                                                                                                                           >  2:1102998/1‑249 (MQ=25)

                                                                           aCAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAacttatg                                                                                                                                                                          >  1:595101/1‑249 (MQ=255)

                                                                            cAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgc                                                                                                                                                                         >  2:234624/1‑249 (MQ=255)

                                                                              aCTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcct                                                                                                                                                                       <  2:1212413/249‑1 (MQ=255)

                                                                                 ttGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgccttat                                                                                                                                                                    <  1:716959/249‑1 (MQ=255)

                                                                                        tttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTAc                                                                                                                                                             <  1:385220/249‑1 (MQ=255)

                                                                                         ttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGCTCAGACAGTATTTAAGACGCTGAACTTTATTCAAGTCCAGATTCCTACCTCACGCCTTATGGTTTaat                                                                                                                                                            >  2:917477/1‑247 (MQ=37)

                                                                                             cTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTcaca                                                                                                                                                        <  2:501620/249‑1 (MQ=255)

                                                                                              tAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACAATGTGTTGAGACAGTATTTAAGACTCTTAACTTTATTACAGTGCAAAGTTCTACTTTATCTCTTATTTTTTACTcacac                                                                                                                                                       >  2:476186/1‑249 (MQ=25)

                                                                                               aaTGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAGACAATACTTAAGACTCTTACCTCTATTCAAGTCCAGATTCTTACCTCTTCCCTTATGTTTTACTcataca                                                                                                                                                      >  2:19238/1‑249 (MQ=25)

                                                                                                 tGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACAcc                                                                                                                                                    <  2:693875/249‑1 (MQ=255)

                                                                                                 tGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACGCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATCCCTTATGGTTTAATcatacatc                                                                                                                                                    >  2:651234/1‑247 (MQ=255)

                                                                                                    ccTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAg                                                                                                                                                 <  1:791190/249‑1 (MQ=255)

                                                                                                       gtaGATTTGTGGGCAAGGAAAGATTCGGGTTATTGTTTATCTCGGGCCAAGGGCAATAAGCCCTAATCACCCAGCCCGTGGTGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTACCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGccc                                                                                                                                              <  2:1110652/247‑1 (MQ=37)

                                                                                                        cAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCg                                                                                                                                             <  2:593412/249‑1 (MQ=255)

                                                                                                            tttGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAGACAATATTTAAGACTCTGAACTTTATTAAAGGCCAGAGTCTTACCTTATCCCTTATGATTTACTCACACATCCAGTCCggggg                                                                                                                                         >  1:925780/1‑247 (MQ=37)

                                                                                                            tttGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCgggcg                                                                                                                                         >  1:425169/1‑247 (MQ=255)

                                                                                                             ttGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTgg                                                                                                                                        <  2:1174901/249‑1 (MQ=255)

                                                                                                               gtgtTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCg                                                                                                                                      >  1:998621/1‑249 (MQ=255)

                                                                                                               gtgtTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCg                                                                                                                                      >  1:560950/1‑249 (MQ=255)

                                                                                                                  ttCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGaa                                                                                                                                   >  1:325232/1‑249 (MQ=255)

                                                                                                                   tCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTACGGTTTACTCACACACCCAGCCCGGGTGGCGGAAt                                                                                                                                  >  2:83671/1‑249 (MQ=255)

                                                                                                                     aaGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATgg                                                                                                                                <  1:1183951/249‑1 (MQ=255)

                                                                                                                        gAAAGGTTTGGGCTATTTTTTATCACGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAg                                                                                                                             <  2:734783/249‑1 (MQ=255)

                                                                                                                             aTTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAGACAGTCTTTAAGACTCTGAACTTTATTCCAGTCCAGAGTCTTACCTTATGCCTGATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGAcgcg                                                                                                                        >  2:888682/1‑249 (MQ=255)

                                                                                                                             aTTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTTAAATAGTATTTTAGACTCTGAACTTTATTCAAGTGTAGATTCTTACTTTTTCTATTGTGTTTTAATAACACATTCTATCCTGGTGGCGGTACGGCAGAcgtg                                                                                                                        >  2:1006500/1‑247 (MQ=18)

                                                                                                                                cGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGGTTAGACCGTATTTAAGACTCTGAACTTTATTAAAGTCCGGTGTCTTACCTTATCTATTATGGTTTAATCACACATCCTACACGGCGACCGGTATGGCAGActtgacn                                                                                                                     >  2:1192757/1‑243 (MQ=21)

                                                                                                                                 gggCTATTTTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAg                                                                                                                    <  2:901650/249‑1 (MQ=255)

                                                                                                                                   gCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCt                                                                                                                  >  2:956236/1‑249 (MQ=255)

                                                                                                                                    cTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGGTTTACTCACACACCCAGTCCGGGTGGCGGAATGGCAGACGTGCTAGCtt                                                                                                                 >  2:139291/1‑249 (MQ=255)

                                                                                                                                      aTTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGa                                                                                                               <  1:361438/249‑1 (MQ=255)

                                                                                                                                      aTTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGa                                                                                                               <  2:661224/249‑1 (MQ=255)

                                                                                                                                          tttATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTg                                                                                                           <  1:963526/249‑1 (MQ=255)

                                                                                                                                                 cGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTTTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCTAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGCCGGAATGGCAGACGCGCTAGCTTGAGTTGCTAgtat                                                                                                    >  2:956216/1‑247 (MQ=255)

                                                                                                                                                          ggCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAAc                                                                                           >  1:1154190/1‑249 (MQ=255)

                                                                                                                                                           gccattaGCCCTGACCACCCCGCGCGGGTGGCGGTGTGGCAGACGCGCTACCTTGAGGTCCTCGTGCCCTATTAACGGACGTGGGGGTTCATGT‑CCCC‑CTC‑CCTGCTCACTGTGTTGAGACAGTATTTAAGACTCAGAACTTTATCCAAGTCCAGAGTCTTACCTGCCGCCTTATGTTTTCCTTACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACg                                                                                          <  2:46922/243‑1 (MQ=25)

                                                                                                                                                               tAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACg                                                                                      >  1:85829/1‑249 (MQ=255)

                                                                                                                                                                     cTAAACACCCAGCCCGGGTGGCTGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTggggg                                                                                <  1:997977/249‑1 (MQ=255)

                                                                                                                                                                       aaaCACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGtt                                                                              >  1:819407/1‑249 (MQ=255)

                                                                                                                                                                          cacCCCGCCCGAGTGGCGGAATGGCAGACGCACTAGCATGAGGGGCTAGTGTCCTATTAACGTACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCaa                                                                           <  2:284110/249‑1 (MQ=255)

                                                                                                                                                                          cacCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCCAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTTCTAGTGTCCTATTAACGGAAGTGGGGGTTCan                                                                           >  2:761726/1‑248 (MQ=255)

                                                                                                                                                                           acCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAg                                                                          >  1:147113/1‑249 (MQ=255)

                                                                                                                                                                                     ggTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTAACACACCCAGCCCGGGTGCCGGAATGGCAGACGCGCTAGCTTTAGGTTCTAGTGTCCTATTAACTGACTTCGGGGTTCACGTCTCCCCCCg                                                                >  2:649374/1‑249 (MQ=37)

                                                                                                                                                                                            gAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACaa                                                         >  2:766492/1‑249 (MQ=255)

                                                                                                                                                                                                    gACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTa                                                 <  1:627199/249‑1 (MQ=255)

                                                                                                                                                                                                     aCGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAAACCGTATTTAAGACTCTTAACTTTATTCAAGTCCAGAGTCTTACTTTATGTATTATTGTTTACTAATACACCCAGTCCGGCTGGCGGAATGGCAGACGTGCCAGCTTGAGGTGCTAGTGTCCTGTTAACGTACGTGTGTGTTCAAGTCCCCCCCCGGGCAACAATTAGttta                                                >  2:246802/1‑247 (MQ=25)

                                                                                                                                                                                                       gcgcTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑CCCC‑C‑CGCGGGCATACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAAt                                              <  1:1222334/249‑1 (MQ=255)

                                                                                                                                                                                                              cTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGTGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTg                                       >  2:299200/1‑249 (MQ=255)

                                                                                                                                                                                                               ttGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCATTATGTTTTACTCACACACTCAACCATGGTGGCGGAATGGCTGACGCGATAGCTTGAGGTGCTAGTGTCCTATTAACGGACTTTGGGGTTTAAGTCCCCCATCGGGCAAAAATTACTTAAATCACCCTGt                                      >  2:639718/1‑249 (MQ=38)

                                                                                                                                                                                                                 gAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTAc                                    <  1:129925/249‑1 (MQ=255)

                                                                                                                                                                                                                  agttGCTAGTGTCCTATTAACTGACGTGGGGGTTCAAGT‑CCCCTC‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTCTATTCACGTCCACTGCCCTACGTTATGCCTTATGTATTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACa                                   <  2:85829/246‑1 (MQ=37)

                                                                                                                                                                                                                     tGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACannn                                >  2:434355/1‑246 (MQ=255)

                                                                                                                                                                                                                     tGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACannn                                >  1:232036/1‑246 (MQ=255)

                                                                                                                                                                                                                        naGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTa                             <  1:782007/248‑1 (MQ=255)

                                                                                                                                                                                                                         aGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTAc                            >  1:964969/1‑249 (MQ=255)

                                                                                                                                                                                                                          gtgtCGTATTAACTGACGTGGGGGTTAAAATAACTT‑C‑G‑CGGGTACACCGAGTTGAGAGAGTATTTAAGACTCTGAAAATTATCGAAGTCCAGAGTCTTACCTTATGCGTTAAGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTAct                           <  2:830742/249‑1 (MQ=25)

                                                                                                                                                                                                                                tATTAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGt                     >  2:109225/1‑249 (MQ=255)

                                                                                                                                                                                                                                  ttAACGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTAc                   <  1:956236/249‑1 (MQ=255)

                                                                                                                                                                                                                                     aCGGACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAgg                >  2:764263/1‑249 (MQ=255)

                                                                                                                                                                                                                                       ggACGTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGt              <  1:374502/249‑1 (MQ=255)

                                                                                                                                                                                                                                           gTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtt          <  1:1178951/249‑1 (MQ=255)

                                                                                                                                                                                                                                           gTGGGGGTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtt          >  1:966603/1‑249 (MQ=255)

                                                                                                                                                                                                                                               gggTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCt      >  2:621610/1‑249 (MQ=255)

                                                                                                                                                                                                                                                ggTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTg     >  1:694322/1‑249 (MQ=255)

                                                                                                                                                                                                                                                ggTTCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTg     >  1:176728/1‑249 (MQ=255)

                                                                                                                                                                                                                                                   tCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGttt  <  1:372033/249‑1 (MQ=255)

                                                                                                                                                                                                                                                   tCAAGTCCCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGttt  >  2:509933/1‑249 (MQ=255)

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGT‑CCCC‑C‑C‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTTT  >  CP025533/1610971‑1611461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`