BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	2,084,508	0	G	T	48.0%	57.4 / inf	252	intergenic (‑10/+191)	dapA/CYL77_09945	4‑hydroxy‑tetrahydrodipicolinate synthase/thymidylate synthase (FAD)
Reads supporting (aligned to +/- strand): ref base G (54/77); new base T (48/73); total (102/150)
Fisher's exact test for biased strand distribution p-value = 8.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

CAACTTCCTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGCCAAAGTTTC  >  CP025533/2084261‑2084755

   cTTCCTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTa                                                                                                                                                                                                                                                       <  2:270596/249‑1 (MQ=255)

          aCGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACcttctt                                                                                                                                                                                                                                                <  1:1131647/249‑1 (MQ=255)

          aCGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACcttctt                                                                                                                                                                                                                                                <  1:1229255/249‑1 (MQ=255)

            gAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTcc                                                                                                                                                                                                                                              <  1:1189249/249‑1 (MQ=255)

              aCGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCt                                                                                                                                                                                                                                            >  1:871520/1‑249 (MQ=255)

                ggCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCa                                                                                                                                                                                                                                          >  2:87995/1‑249 (MQ=255)

                  ccTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑tt                                                                                                                                                                                                                                       <  1:352692/249‑1 (MQ=255)

                   cTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑ttt                                                                                                                                                                                                                                      >  2:1097453/1‑249 (MQ=255)

                    ntGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑tttt                                                                                                                                                                                                                                     <  1:1160409/248‑2 (MQ=255)

                        gCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTT‑tgggg                                                                                                                                                                                                                                >  2:805671/1‑249 (MQ=255)

                            ttCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTaa                                                                                                                                                                                                                            <  2:673937/249‑1 (MQ=255)

                              nnaGTTTTTCAGCGGCGGGTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTaaa                                                                                                                                                                                                                           <  1:1174322/247‑1 (MQ=255)

                               tAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAaaca                                                                                                                                                                                                                         >  1:1244426/1‑249 (MQ=255)

                                  tttttCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAaacaac                                                                                                                                                                                                                       >  2:17589/1‑249 (MQ=255)

                                  tttttCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAAcc                                                                                                                                                                                                                      >  1:579315/1‑249 (MQ=255)

                                            gcggTTGTCGTTGGGGATTCACCAGTGATGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCaaaaaa                                                                                                                                                                                                            >  2:164346/1‑249 (MQ=255)

                                              ggTTGTCGTTGTGGAATCACCAGTGGTCCCGGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGTTTACCTTCTTCCCTCA‑TTT‑TGGGGTTAAACAACCCCGCAAAAAAcc                                                                                                                                                                                                          <  2:196176/249‑1 (MQ=255)

                                                  gTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTaa                                                                                                                                                                                                       <  1:135292/249‑1 (MQ=255)

                                                     gTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTaacaac                                                                                                                                                                                                   >  2:1086010/1‑249 (MQ=255)

                                                       tGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACtt                                                                                                                                                                                                 <  2:500694/249‑1 (MQ=255)

                                                       tGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACtt                                                                                                                                                                                                 <  2:755515/249‑1 (MQ=255)

                                                         gggATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACttt                                                                                                                                                                                                <  2:992495/249‑1 (MQ=255)

                                                          ggATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACtttt                                                                                                                                                                                               >  2:557305/1‑249 (MQ=255)

                                                             ttCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCa                                                                                                                                                                                            >  1:1005926/1‑249 (MQ=255)

                                                              tCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATa                                                                                                                                                                                          >  1:255977/1‑249 (MQ=255)

                                                               cACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATa                                                                                                                                                                                          <  1:1125431/249‑1 (MQ=255)

                                                                 ccAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAAc                                                                                                                                                                                        <  1:235920/249‑1 (MQ=255)

                                                                     tggtgCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATc                                                                                                                                                                                    <  2:1171564/249‑1 (MQ=255)

                                                                     tggtgCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCg                                                                                                                                                                                   <  1:912428/249‑1 (MQ=255)

                                                                      ggtgCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGc                                                                                                                                                                                  <  1:664587/249‑1 (MQ=255)

                                                                            cgcgAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTACACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAg                                                                                                                                                                             >  1:290545/1‑249 (MQ=255)

                                                                             gcgAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGc                                                                                                                                                                            <  2:1013067/249‑1 (MQ=255)

                                                                                  aaCCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACAtt                                                                                                                                                                      >  2:1108211/1‑249 (MQ=255)

                                                                                   aCCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACAtt                                                                                                                                                                      <  1:669832/249‑1 (MQ=255)

                                                                                     cAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTcc                                                                                                                                                                    <  2:302602/249‑1 (MQ=255)

                                                                                      aaaGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGa                                                                                                                                                                  <  1:702585/249‑1 (MQ=255)

                                                                                        aGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATa                                                                                                                                                                <  1:507389/249‑1 (MQ=255)

                                                                                          aaTCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATaa                                                                                                                                                               <  1:31021/249‑1 (MQ=255)

                                                                                           aTCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGa                                                                                                                                                             <  1:994549/249‑1 (MQ=255)

                                                                                            tCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGaa                                                                                                                                                            <  1:1074089/249‑1 (MQ=255)

                                                                                              cAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAAc                                                                                                                                                           <  1:852855/249‑1 (MQ=255)

                                                                                               aaGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTCTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACaa                                                                                                                                                         >  2:641189/1‑249 (MQ=255)

                                                                                                  cccTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAAtt                                                                                                                                                       <  1:1225396/249‑1 (MQ=255)

                                                                                                  cccTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACC‑TCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTg                                                                                                                                                      >  1:26467/1‑249 (MQ=255)

                                                                                                    cTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCTCCCAAGCCACATTCCGATAAGAACAATTGa                                                                                                                                                     >  1:316473/1‑249 (MQ=255)

                                                                                                    cTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGac                                                                                                                                                    <  1:1086010/249‑1 (MQ=255)

                                                                                                      tATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGaca                                                                                                                                                   <  2:612747/249‑1 (MQ=255)

                                                                                                          aaCCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGc                                                                                                                                              <  2:1071051/249‑1 (MQ=255)

                                                                                                            ccAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCa                                                                                                                                             >  2:775629/1‑249 (MQ=255)

                                                                                                              aaaTAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAaga                                                                                                                                          <  1:468923/249‑1 (MQ=255)

                                                                                                                  aaGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGtt                                                                                                                                      >  2:283033/1‑249 (MQ=255)

                                                                                                                   aGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGttt                                                                                                                                     >  2:699754/1‑249 (MQ=255)

                                                                                                                     ccGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGttttt                                                                                                                                   >  2:534689/1‑249 (MQ=255)

                                                                                                                          aCTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgt                                                                                                                               <  2:1024552‑M2/249‑10 (MQ=255)

                                                                                                                                       cagcGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGc                                                                                                                 <  1:148612/249‑1 (MQ=255)

                                                                                                                                        agcGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGcc                                                                                                                >  1:114917/1‑249 (MQ=255)

                                                                                                                                           gATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCaaa                                                                                                             >  1:600921/1‑249 (MQ=255)

                                                                                                                                             tatCGCTGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTATGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCaaaaa                                                                                                           >  2:1230168/1‑249 (MQ=255)

                                                                                                                                             tatCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCaaaaa                                                                                                           <  2:222036/249‑1 (MQ=255)

                                                                                                                                               tCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagac                                                                                                          <  2:723523‑M2/249‑31 (MQ=255)

                                                                                                                                               tCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAAtg                                                                                                         <  2:749217/249‑1 (MQ=255)

                                                                                                                                                   tGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggcc                                                                                                      <  1:383873‑M2/249‑35 (MQ=255)

                                                                                                                                                   tGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggcc                                                                                                      >  1:641364‑M2/1‑215 (MQ=255)

                                                                                                                                                         cGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggc                                                                                                <  2:416198‑M2/249‑41 (MQ=255)

                                                                                                                                                               ccGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgatgct                                                                                          <  2:323733‑M2/249‑47 (MQ=255)

                                                                                                                                                                 gTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGaa                                                                                       <  1:1065555/249‑1 (MQ=255)

                                                                                                                                                                  tGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAt                                                                                      >  2:938574/1‑249 (MQ=255)

                                                                                                                                                                     aTGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaag                                                                                    <  1:44843‑M2/249‑53 (MQ=255)

                                                                                                                                                                     aTGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAtttt                                                                                   <  2:442187/249‑1 (MQ=255)

                                                                                                                                                                     aTGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAtttt                                                                                   >  1:1017147/1‑249 (MQ=255)

                                                                                                                                                                       ggAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAtttttn                                                                                 >  2:685503/1‑248 (MQ=255)

                                                                                                                                                                                    cTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggc                                                                     <  1:49523‑M2/249‑68 (MQ=255)

                                                                                                                                                                                    cTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgcatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggc                                                                     >  2:592061‑M2/1‑182 (MQ=255)

                                                                                                                                                                                    cTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGtgtg                                                                    <  1:723370/249‑1 (MQ=255)

                                                                                                                                                                                     tACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGtgtgn                                                                   >  1:638139/1‑248 (MQ=255)

                                                                                                                                                                                     nacTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCTCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcg                                                                    >  2:316473‑M2/2‑181 (MQ=255)

                                                                                                                                                                                        tCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccg                                                                 <  1:906502‑M2/249‑72 (MQ=255)

                                                                                                                                                                                        tCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCt                                                                >  1:586467/1‑249 (MQ=255)

                                                                                                                                                                                          cAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCttt                                                              <  2:427583/249‑1 (MQ=255)

                                                                                                                                                                                                tGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTa                                                        >  1:704609/1‑249 (MQ=255)

                                                                                                                                                                                                tGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTa                                                        >  2:1047082/1‑249 (MQ=255)

                                                                                                                                                                                                  ccGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTaaa                                                      >  2:729309/1‑249 (MQ=255)

                                                                                                                                                                                                   cGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagttttt                                                      >  2:1148090‑M2/1‑167 (MQ=255)

                                                                                                                                                                                                     aaGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttat                                                    <  2:1251036‑M2/249‑85 (MQ=255)

                                                                                                                                                                                                            nnactACGGTCTTAGCTGTTAAACCTGTTCTCATAGAGTTCAATTATACCATCTTCTCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTCTGCATAACATTCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcc                                             >  2:402610‑M2/3‑158 (MQ=255)

                                                                                                                                                                                                                tCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATAc                                        >  1:1093211/1‑249 (MQ=255)

                                                                                                                                                                                                                   ggTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgcc                                      >  1:805671‑M2/1‑151 (MQ=255)

                                                                                                                                                                                                                      cTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaa                                   <  1:1255489‑M2/249‑102 (MQ=255)

                                                                                                                                                                                                                       ttAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCAAAAAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattggatcacaccgccgaac                                  <  2:458001‑M2/249‑103 (MQ=255)

                                                                                                                                                                                                                       ntAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCttg                                 <  1:322444/248‑1 (MQ=255)

                                                                                                                                                                                                                       ntAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCttg                                 <  2:1219742/248‑1 (MQ=255)

                                                                                                                                                                                                                          gCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCttgttg                              >  1:212274/1‑249 (MQ=255)

                                                                                                                                                                                                                              ttAAACCTGTGCTCATACAGTTCAATGATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatat                           >  1:172740‑M2/1‑140 (MQ=255)

                                                                                                                                                                                                                                 aaCCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcg                        <  2:1194276‑M2/249‑113 (MQ=255)

                                                                                                                                                                                                                                  aCCAGTGCTCATAGAGTTCAAGGTTACCTTCTACCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCTATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGc                      <  2:865065/249‑1 (MQ=255)

                                                                                                                                                                                                                                        gCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttgtattgggtcccaccgccgaactgcatattcgaaccaag                 <  1:777904‑M2/249‑120 (MQ=255)

                                                                                                                                                                                                                                           cATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCt             >  1:736775/1‑249 (MQ=255)

                                                                                                                                                                                                                                              agagTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcgaaccaaggagcct           <  2:919319‑M2/249‑126 (MQ=255)

                                                                                                                                                                                                                                              agagTTCAATTATACCT‑CTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcgaaccaaggagcctc          >  2:26467‑M2/1‑123 (MQ=255)

                                                                                                                                                                                                                                              agagTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGcc          <  2:888948/249‑1 (MQ=255)

CAACTTCCTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGCCAAAGTTTC  >  CP025533/2084261‑2084755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`