BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP025533	1,611,226	(C)_7→8	noncoding (80/85 nt)	CYL77_07745 →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	1,611,219	1	.	C	86.8%	422.5 / 49.7	129	noncoding (73/85 nt)	CYL77_07745	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (2/15); new base C (48/64); total (50/79)
Fisher's exact test for biased strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑C‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTTTC  >  CP025533/1610971‑1611462

aTCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑t                                                                                                                                                                                                                                                           >  1:1412221/1‑249 (MQ=255)

   cTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑c‑c‑c                                                                                                                                                                                                                                                     <  2:51983/249‑1 (MQ=255)

   cTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGCTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑c‑c‑c                                                                                                                                                                                                                                                     >  2:362031/1‑249 (MQ=255)

     ggggAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑c‑c‑cc‑c                                                                                                                                                                                                                                                  <  2:536704/249‑1 (MQ=255)

        gAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGAGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑c‑c‑cc‑c‑ccc                                                                                                                                                                                                                                              >  2:940920/1‑248 (MQ=255)

          aTTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑C‑C‑CC‑C‑CC‑cgg                                                                                                                                                                                                                                           >  1:400094/1‑249 (MQ=255)

                       ggCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTg                                                                                                                                                                                                                               <  1:265823/249‑1 (MQ=255)

                         cGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTgag                                                                                                                                                                                                                             <  1:19218/249‑1 (MQ=255)

                         cGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTgag                                                                                                                                                                                                                             <  2:159930/249‑1 (MQ=255)

                          gAAAACACTACCGCAGCTCTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTgaga                                                                                                                                                                                                                            <  1:628436/249‑1 (MQ=255)

                            aaaCACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACa                                                                                                                                                                                                                          <  2:598785/249‑1 (MQ=255)

                                acTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTAt                                                                                                                                                                                                                      <  2:112062/249‑1 (MQ=255)

                                 cTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTAtt                                                                                                                                                                                                                     <  1:138332/249‑1 (MQ=255)

                                 cTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTAtt                                                                                                                                                                                                                     >  1:495044/1‑249 (MQ=255)

                                  naCCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTATAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTAttt                                                                                                                                                                                                                    <  2:47446/248‑1 (MQ=255)

                                  naCCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTAttt                                                                                                                                                                                                                    <  1:812132/248‑1 (MQ=255)

                                    ccGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTaa                                                                                                                                                                                                                  <  2:1127124/249‑1 (MQ=255)

                                    ccGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTaa                                                                                                                                                                                                                  <  1:1499198/249‑1 (MQ=255)

                                       cAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGAc                                                                                                                                                                                                               >  1:1509628/1‑249 (MQ=255)

                                        aGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGAct                                                                                                                                                                                                              >  1:85438/1‑249 (MQ=255)

                                             tataCGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGaa                                                                                                                                                                                                         <  1:469292/249‑1 (MQ=255)

                                              ataCGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAAc                                                                                                                                                                                                        <  2:335556/249‑1 (MQ=255)

                                               taCGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACt                                                                                                                                                                                                       <  1:112314/249‑1 (MQ=255)

                                                aCGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTTTGATGAGACAGTTTTTGACACTTTGAACtt                                                                                                                                                                                                      >  1:621212/1‑249 (MQ=255)

                                                aCGGACAGTACTTACAAAGCCGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACtt                                                                                                                                                                                                      <  1:1247272/249‑1 (MQ=255)

                                                   gACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTAt                                                                                                                                                                                                   <  1:819924/249‑1 (MQ=255)

                                                     cAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTc                                                                                                                                                                                                 <  1:224767/249‑1 (MQ=255)

                                                      aGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCa                                                                                                                                                                                                <  1:1043112/249‑1 (MQ=255)

                                                       gTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCaa                                                                                                                                                                                               <  2:1073331/249‑1 (MQ=255)

                                                        tACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAg                                                                                                                                                                                              >  1:904695/1‑249 (MQ=255)

                                                              cAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCaga                                                                                                                                                                                        <  1:1140665/249‑1 (MQ=255)

                                                              cAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCaga                                                                                                                                                                                        <  1:460866/249‑1 (MQ=255)

                                                               aaaGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCagag                                                                                                                                                                                       >  1:601499/1‑249 (MQ=255)

                                                                aaGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGt                                                                                                                                                                                      <  2:955083/249‑1 (MQ=255)

                                                                  gCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCt                                                                                                                                                                                    <  1:730966/249‑1 (MQ=255)

                                                                     gTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAc                                                                                                                                                                                 <  2:1341863/249‑1 (MQ=255)

                                                                      tAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcc                                                                                                                                                                                >  1:1161179/1‑249 (MQ=255)

                                                                           aCAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatg                                                                                                                                                                           >  1:1213163/1‑249 (MQ=255)

                                                                           aCAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatg                                                                                                                                                                           <  2:1061539/249‑1 (MQ=255)

                                                                            cAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTAAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgc                                                                                                                                                                          <  2:278894/249‑1 (MQ=255)

                                                                              aCTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcct                                                                                                                                                                        <  1:1254424/249‑1 (MQ=255)

                                                                               cTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcctt                                                                                                                                                                       <  2:872203/249‑1 (MQ=255)

                                                                                 ttGTC‑TTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACAGTGTTGAGACAGTAATTAAGACTATGAAGTTAATTGAAGTCGAGAGTCTTAccttatgccttatg                                                                                                                                                                    >  2:100046/1‑249 (MQ=37)

                                                                                    tCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGAAAGTACTTTAGACTCTGAACTTTATTACAGTCCAAAGTCTTACCTTATGCCTTATGtt                                                                                                                                                                  >  1:996453/1‑249 (MQ=255)

                                                                                                 tGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACAcc                                                                                                                                                     >  2:698428/1‑249 (MQ=255)

                                                                                                 tGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAAACAGTATTTAAGAATCTTAACTTTATTCAAGTCCAGAGTCTTACCTTATCCCTTATTTTTTAATaacacatt                                                                                                                                                     >  2:642923/1‑247 (MQ=37)

                                                                                                   aCCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCa                                                                                                                                                   <  2:351661/249‑1 (MQ=255)

                                                                                                     cTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACATTGGTGACACACTACTTAACACGCTTAACTTTATTACAGTCCATGTTCCTACCTTTTTCCCTATGGTttacttactccacgtac                                                                                                                                                 >  2:1105176/1‑240 (MQ=21)

                                                                                                        cAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGGTTAGACAGTATTTTAGACTCTGAACTTTATTCAAGTCCGAAGTTTTACCTTATGTATTATGTTTTACTCACGCACCCTGTCCg                                                                                                                                              >  2:468127/1‑249 (MQ=25)

                                                                                                         aGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCgg                                                                                                                                             <  2:378532/249‑1 (MQ=255)

                                                                                                         aGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACGCTGAACTTTTTTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTGACACATCCAGCCCgg                                                                                                                                             >  1:1335282/1‑249 (MQ=255)

                                                                                                          gATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCggg                                                                                                                                            <  1:858097/249‑1 (MQ=255)

                                                                                                             ttGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTgg                                                                                                                                         <  2:707092/249‑1 (MQ=255)

                                                                                                                   tCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAAt                                                                                                                                   <  2:169596/249‑1 (MQ=255)

                                                                                                                    cAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTATCGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATg                                                                                                                                  >  1:484133/1‑249 (MQ=255)

                                                                                                                          aaGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGAc                                                                                                                            <  2:790618/249‑1 (MQ=255)

                                                                                                                          aaGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGAc                                                                                                                            <  1:1070405/249‑1 (MQ=255)

                                                                                                                             aTTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGAcgcg                                                                                                                         >  1:123769/1‑249 (MQ=255)

                                                                                                                                cGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTa                                                                                                                      <  1:135914/249‑1 (MQ=255)

                                                                                                                                cGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTa                                                                                                                      <  1:698428/249‑1 (MQ=255)

                                                                                                                                   gCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCt                                                                                                                   >  2:551868/1‑249 (MQ=255)

                                                                                                                                           ttATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGc                                                                                                           >  1:528713/1‑249 (MQ=255)

                                                                                                                                                ncGTGCCAAGGGCAATAAGCCTAAAACACCCGGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGCGTTC‑AAG‑T‑CTC‑CC‑C‑CC‑GGGCAGAGTGTGTTGAGACAGTATTTAAGGCTCTGAACTTTATTCAAGTCCAGGTTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAgtg                                                                                                      <  1:1002803/248‑1 (MQ=37)

                                                                                                                                                   tGCCAAGGGCAATAAGCCCTAAAGACCCAGGCCGGGTGGAGGAATGGTAGAGGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGTACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGTCTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTCTGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTcc                                                                                                   <  2:1037607/249‑1 (MQ=38)

                                                                                                                                                       aaGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACTCTGCCCGGGTGGCGGTATGGCAGACGCGCTAGCTTTATGTTCTAGTGTCCTAtt                                                                                               >  2:894152/1‑249 (MQ=255)

                                                                                                                                                       aaGGGCAATAAGCCCGAAACACCCCGGCCGGGTGGAGGAATGGCGGACGAGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCGAAT‑A‑C‑C‑CC‑T‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCAGAACAGTATTCAAGTCCAGTGTCTTACCTGATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTAtt                                                                                               <  2:774343/249‑1 (MQ=37)

                                                                                                                                                                 aGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACAGTGTTTAGATAGTATTTTAGACTCTGAACTTTATTCAAGGGCAGAGTCTTACCTTATCTCTTATTGTTTACAAACACACTCTGTACTGCGGGCGGTATGGCAGATTTGCTTGCTTGGGGTTCGAGTATCCAGTTAActtatgtc                                                                                     >  2:824938/1‑242 (MQ=12)

                                                                                                                                                                   cccTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTggg                                                                                   >  1:288782/1‑249 (MQ=255)

                                                                                                                                                                       aaaCACCCAGGCCGGGTGGCTGAATGGCAGCCGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACTGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGtt                                                                               <  2:445370/249‑1 (MQ=255)

                                                                                                                                                                        aaCACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTGGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGGGCCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAATGTCCTATTAACGGACGTGGGGGTTc                                                                              >  1:615343/1‑249 (MQ=255)

                                                                                                                                                                            cccAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGt                                                                          <  1:1447990/249‑1 (MQ=255)

                                                                                                                                                                                    gggTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAACCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGTTGCTAGTGTCCTGTTAACGGACTTCGGGGTTCAAGTcccccccc                                                                  >  2:841966/1‑249 (MQ=255)

                                                                                                                                                                                      gTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTTAGACAGTATTTAAGACGCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCgg                                                                >  2:335730/1‑249 (MQ=255)

                                                                                                                                                                                              aTGGCAGACGCGCTAGCTTGAGGTGCTTGTGTCCTAATAACGGACGTGGCGGTTC‑AAGCC‑C‑C‑CC‑C‑CC‑GGGCACACCGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAAt                                                        <  1:1228910/249‑1 (MQ=255)

                                                                                                                                                                                               tGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAAtt                                                       >  1:214596/1‑249 (MQ=255)

                                                                                                                                                                                                  cagccgagacaGCTCGAGATGCTAATATGCAATTAACGGACGTGGCTGTTC‑AAA‑T‑C‑C‑CC‑CTCC‑GGGCTTACTGTATTGAGTCAGTATTTAAGACTAAGAAATTTATTCAAGTCCAGAGTCTTACCTTCTGCGTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGt                                                    <  2:350048/239‑1 (MQ=21)

                                                                                                                                                                                                   aGACGCGCTAGCTTGAGGTGCTTGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑C‑C‑CC‑CTCC‑GGGCACACTGTGTTGAGACAGTATTTAAGTCTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGtt                                                   <  1:100046/249‑1 (MQ=255)

                                                                                                                                                                                                   aGACGAGCTAGCTTGAGGTGCTAGTGTCCTATGAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCAAACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGtt                                                   <  1:335624/249‑1 (MQ=255)

                                                                                                                                                                                                     aCGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTaa                                                 <  2:1509628/249‑1 (MQ=255)

                                                                                                                                                                                                      cgcgCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTaaa                                                <  1:877206/249‑1 (MQ=255)

                                                                                                                                                                                                             gCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCt                                         >  1:1474593/1‑249 (MQ=255)

                                                                                                                                                                                                                    gTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAg                                  >  1:1258238/1‑249 (MQ=255)

                                                                                                                                                                                                                     tGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACannn                                 >  1:435002/1‑246 (MQ=255)

                                                                                                                                                                                                                        tAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTa                              >  1:426598/1‑249 (MQ=255)

                                                                                                                                                                                                                        naGTGTCCTATTCACGGACGTGGGGGTTC‑AAG‑T‑C‑C‑CCTC‑CC‑GGGCACACTGTATTAAGACAGGATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTa                              <  1:950296/248‑1 (MQ=255)

                                                                                                                                                                                                                         aatatgcTATGAACGGACGTGGGGGTTT‑GAG‑T‑CAC‑CC‑C‑CC‑GGGCACACTGTGTTGAGAGAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGTGTCTTACCTTATGCCTTAAGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTAc                             <  2:834445/243‑1 (MQ=37)

                                                                                                                                                                                                                           tgtCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTActt                           >  1:739842/1‑249 (MQ=255)

                                                                                                                                                                                                                            gtCCTATTAACGGCAGTGGGGGTTC‑CAG‑T‑C‑CTCC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGTCTATGAACTTAATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTActtc                          <  2:316376/249‑1 (MQ=37)

                                                                                                                                                                                                                            gtCCTATTAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTActtc                          >  1:1000879/1‑249 (MQ=255)

                                                                                                                                                                                                                               cTATTAACGGACGTGGGGGTTC‑AAG‑T‑CAC‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTg                       <  2:883677/249‑1 (MQ=255)

                                                                                                                                                                                                                                  ttAACGGACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTAc                    >  1:606250/1‑249 (MQ=255)

                                                                                                                                                                                                                                   tAACGTACGTGGGTGTTC‑AAA‑C‑CAC‑TT‑C‑CC‑GGTCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACa                   <  2:383468/249‑1 (MQ=37)

                                                                                                                                                                                                                                        gACGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTg              <  1:331454/249‑1 (MQ=255)

                                                                                                                                                                                                                                         aCGTGGGGGTTA‑AAG‑T‑C‑C‑CC‑C‑CCTGGGTATACTTTGTTGAGACAGTATTTAAGACTCTGAACTTTATCCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGa             <  2:601499/249‑1 (MQ=255)

                                                                                                                                                                                                                                          cGTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAt            >  1:1389336/1‑249 (MQ=255)

                                                                                                                                                                                                                                           gTGGGGGTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtt           >  2:247130/1‑249 (MQ=255)

                                                                                                                                                                                                                                             gggggTTCTAGG‑C‑C‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTGTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGGGGGGGAATGGCAGACGCGCTAGGTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtttt         <  1:887321/249‑1 (MQ=255)

                                                                                                                                                                                                                                              ggggTTC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGTGGGTTCAAGTCCCCCCCCGGGCCCAAATTAGTTAAATCACACTGTACAGTATTACTTCTTGTACAGGGTGCttttt        >  2:1257837/1‑248 (MQ=38)

                                                                                                                                                                                                                                                   tC‑AAG‑TCC‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGttt   <  1:439825/249‑1 (MQ=255)

                                                                                                                                                                                                                                                    c‑AAG‑T‑C‑C‑CC‑CTCC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTTtc  <  1:1330623/249‑1 (MQ=255)

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTC‑AAG‑T‑C‑C‑CC‑C‑CC‑GGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTTTC  >  CP025533/1610971‑1611462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`