BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP025533	1,244,414	T→C	V59A (GTT→GCT)	CYL77_05985 →	homoserine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	1,244,414	0	T	C	99.4%	636.7 / NA	158	V59A (GTT→GCT)	CYL77_05985	homoserine dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (71/86); minor base G (1/0); total (72/86)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

GGACACATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGTTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCTGT  >  CP025533/1244168‑1244659

  acacATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCt                                                                                                                                                                                                                                                   >  2:1161415/1‑249 (MQ=255)

  acacATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCt                                                                                                                                                                                                                                                   >  2:1205551/1‑249 (MQ=255)

      aTGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGata                                                                                                                                                                                                                                               <  1:438906/249‑1 (MQ=255)

      aTGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCAGCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGata                                                                                                                                                                                                                                               <  2:1151044/249‑1 (MQ=255)

        gTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATAtc                                                                                                                                                                                                                                             <  1:695985/249‑1 (MQ=255)

         tAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATAtct                                                                                                                                                                                                                                            <  1:350013/249‑1 (MQ=255)

           gggTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCa                                                                                                                                                                                                                                          >  2:753916/1‑249 (MQ=255)

                 ccGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCa                                                                                                                                                                                                                                    >  1:778840/1‑249 (MQ=255)

                 ccGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCa                                                                                                                                                                                                                                    <  2:910614/249‑1 (MQ=255)

                 ccGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCa                                                                                                                                                                                                                                    >  2:147386/1‑249 (MQ=255)

                    aaaCAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGt                                                                                                                                                                                                                                 <  1:290404/249‑1 (MQ=255)

                     aaCAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTg                                                                                                                                                                                                                                <  2:974061/249‑1 (MQ=255)

                       cAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGan                                                                                                                                                                                                                              >  2:470119/1‑248 (MQ=255)

                        aaaGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAg                                                                                                                                                                                                                             <  2:695013/249‑1 (MQ=255)

                             aaTAGGACAACAACGCTCGACTGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGGTTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGtt                                                                                                                                                                                                                        <  1:1239788/249‑1 (MQ=255)

                             aaTAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGtt                                                                                                                                                                                                                        <  1:1463725/249‑1 (MQ=255)

                             aaTAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGtt                                                                                                                                                                                                                        >  1:347299/1‑249 (MQ=255)

                              aTAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTg                                                                                                                                                                                                                       <  1:41172/249‑1 (MQ=255)

                               tAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCAGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGc                                                                                                                                                                                                                      >  2:617759/1‑249 (MQ=255)

                                    caacaaCGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTg                                                                                                                                                                                                                 >  1:13857/1‑249 (MQ=255)

                                            cTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTc                                                                                                                                                                                                         >  1:1397508/1‑249 (MQ=255)

                                             tCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCa                                                                                                                                                                                                        >  1:483285/1‑249 (MQ=255)

                                              cGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCAc                                                                                                                                                                                                       <  1:625244/249‑1 (MQ=255)

                                                aCCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTg                                                                                                                                                                                                     <  2:134672/249‑1 (MQ=255)

                                                aCCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTg                                                                                                                                                                                                     >  1:705682/1‑249 (MQ=255)

                                                       ttattTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGc                                                                                                                                                                                              >  1:3111/1‑249 (MQ=255)

                                                         attTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCTGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCtt                                                                                                                                                                                            >  1:392108/1‑249 (MQ=255)

                                                         attTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCtt                                                                                                                                                                                            >  1:95874/1‑249 (MQ=255)

                                                          tttttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCttt                                                                                                                                                                                           <  1:1068655/249‑1 (MQ=255)

                                                            tttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTg                                                                                                                                                                                         <  1:1156472/249‑1 (MQ=255)

                                                             ttGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGc                                                                                                                                                                                        >  1:72803/1‑249 (MQ=255)

                                                                     tCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCg                                                                                                                                                                                >  2:922805/1‑249 (MQ=255)

                                                                        tGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAgc                                                                                                                                                                             <  1:1176804/249‑1 (MQ=255)

                                                                         gACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAgcg                                                                                                                                                                            >  1:721987/1‑249 (MQ=255)

                                                                             tCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAg                                                                                                                                                                        >  1:496258/1‑249 (MQ=255)

                                                                                 cATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATg                                                                                                                                                                    <  2:1037576/249‑1 (MQ=255)

                                                                                  aTCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGt                                                                                                                                                                   >  1:611574/1‑249 (MQ=255)

                                                                                   nnngCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGtt                                                                                                                                                                  <  1:925757/246‑1 (MQ=255)

                                                                                   nnngCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGtt                                                                                                                                                                  <  1:1029615/246‑1 (MQ=255)

                                                                                    nngCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTg                                                                                                                                                                 <  2:1020425/247‑1 (MQ=255)

                                                                                    nngCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTg                                                                                                                                                                 <  1:206609/247‑1 (MQ=255)

                                                                                          cAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcg                                                                                                                                                           >  1:909705/1‑249 (MQ=255)

                                                                                          cAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcg                                                                                                                                                           >  1:1021905/1‑249 (MQ=255)

                                                                                            aGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcgtc                                                                                                                                                         <  2:1196099/249‑1 (MQ=255)

                                                                                             gCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACAtcgtcg                                                                                                                                                        >  1:1438353/1‑249 (MQ=255)

                                                                                               nttAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGtt                                                                                                                                                      <  2:1471406/248‑1 (MQ=255)

                                                                                                    ccccGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGt                                                                                                                                                 >  2:1288357/1‑249 (MQ=255)

                                                                                                    ccccGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGt                                                                                                                                                 <  1:331593/249‑1 (MQ=255)

                                                                                                     cccGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGtt                                                                                                                                                <  2:137289/249‑1 (MQ=255)

                                                                                                      ccGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTa                                                                                                                                               <  2:1209144/249‑1 (MQ=255)

                                                                                                        ggCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATc                                                                                                                                             >  2:625904/1‑249 (MQ=255)

                                                                                                        ggCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATc                                                                                                                                             <  1:232429/249‑1 (MQ=255)

                                                                                                               gTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGGACAGTCGGCACTGAGGTGATGCGTCTGGTGGCCGAGTACGGTGATGGACTTGCGCAACGCCTTGGGGGCCCACTGGGGGTTCGTGGCATTGCTGGTTCTGATATCTCAAAGCCACGGGGAGGCGTTGGAACTGAGCTGCTTACTTGGGGCGCTTTTTCCCTCATCGAGCGCGGGGGTGTTGGCAACGGCGTTGAGGTTATcggggggn                                                                                                                                      >  2:56943/1‑247 (MQ=255)

                                                                                                                tCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCAt                                                                                                                                     >  1:163052/1‑249 (MQ=255)

                                                                                                                tCCCGGCTCAGCAGTCGGAATTACCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCAt                                                                                                                                     <  1:545583/249‑1 (MQ=255)

                                                                                                                     gCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGt                                                                                                                                >  2:914340/1‑249 (MQ=255)

                                                                                                                        cagcagTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTAcc                                                                                                                             <  2:72803/249‑1 (MQ=255)

                                                                                                                        cagcagTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTAcc                                                                                                                             <  2:1130932/249‑1 (MQ=255)

                                                                                                                                   aTTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTa                                                                                                                  <  2:353685/249‑1 (MQ=255)

                                                                                                                                      gCCCTTTTAGGATTCGGAACATTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGtt                                                                                                               <  2:595208/249‑1 (MQ=255)

                                                                                                                                                 aTTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGctct                                                                                                    <  2:537773/249‑1 (MQ=255)

                                                                                                                                                 aTTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGctct                                                                                                    >  1:337307/1‑249 (MQ=255)

                                                                                                                                                    cGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGaa                                                                                                 <  2:808763/249‑1 (MQ=255)

                                                                                                                                                      gAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAgg                                                                                               <  2:23578/249‑1 (MQ=255)

                                                                                                                                                       aaCAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGc                                                                                              >  1:611693/1‑249 (MQ=255)

                                                                                                                                                       aaCAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGc                                                                                              <  2:1401676/249‑1 (MQ=255)

                                                                                                                                                                cACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTc                                                                                     <  1:955144/249‑1 (MQ=255)

                                                                                                                                                                    gAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCtgtt                                                                                 <  2:569626/249‑1 (MQ=255)

                                                                                                                                                                     aGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCtgttg                                                                                >  1:539288/1‑249 (MQ=255)

                                                                                                                                                                       gTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTgttgtt                                                                              <  2:169936/249‑1 (MQ=255)

                                                                                                                                                                           tGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCg                                                                          <  2:705682/249‑1 (MQ=255)

                                                                                                                                                                            gCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGc                                                                         <  1:872202/249‑1 (MQ=255)

                                                                                                                                                                              gTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCa                                                                       <  1:1288357/249‑1 (MQ=255)

                                                                                                                                                                               tCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCaa                                                                      >  1:1412337/1‑249 (MQ=255)

                                                                                                                                                                                   atgaCCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAg                                                                  <  1:79268/249‑1 (MQ=255)

                                                                                                                                                                                   atgaCCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAg                                                                  >  2:726514/1‑249 (MQ=255)

                                                                                                                                                                                    tgaCCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAgg                                                                 <  1:833255/249‑1 (MQ=255)

                                                                                                                                                                                       ccGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGctc                                                              >  1:1004225/1‑249 (MQ=255)

                                                                                                                                                                                          aGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCttg                                                           <  2:674793/249‑1 (MQ=255)

                                                                                                                                                                                             aCGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCttgttg                                                        >  1:260052/1‑249 (MQ=255)

                                                                                                                                                                                               ggTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCa                                                      <  2:1316650/249‑1 (MQ=255)

                                                                                                                                                                                                gTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAg                                                     <  2:13857/249‑1 (MQ=255)

                                                                                                                                                                                                    tgaACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCa                                                 <  2:1256723/249‑1 (MQ=255)

                                                                                                                                                                                                          tGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTctgc                                           >  2:1077729/1‑249 (MQ=255)

                                                                                                                                                                                                            cgcACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTctgctg                                         <  1:196341/249‑1 (MQ=255)

                                                                                                                                                                                                             gcACCGCATTGGTGGTCCACTGGAGGTTCGTGGCATTGCTGCTGCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGGGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGa                                        <  2:1026570/249‑1 (MQ=255)

                                                                                                                                                                                                                ccGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCt                                     >  1:1256058/1‑249 (MQ=255)

                                                                                                                                                                                                                 cGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCtt                                    >  1:475203/1‑249 (MQ=255)

                                                                                                                                                                                                                   cATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGc                                  >  1:1311602/1‑249 (MQ=255)

                                                                                                                                                                                                                    aTTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCt                                 <  2:332392/249‑1 (MQ=255)

                                                                                                                                                                                                                         tggCCCACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGc                            <  2:10638/249‑1 (MQ=255)

                                                                                                                                                                                                                            cccACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGc                         <  1:922805/249‑1 (MQ=255)

                                                                                                                                                                                                                              cACTGGAGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGCGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCgg                       >  2:546066/1‑249 (MQ=255)

                                                                                                                                                                                                                                    aGGTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCg                 >  2:1325253/1‑249 (MQ=255)

                                                                                                                                                                                                                                     ggTTCGTGGCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGc                <  1:1197431/249‑1 (MQ=255)

                                                                                                                                                                                                                                             gCATTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTg        >  1:1009463/1‑249 (MQ=255)

                                                                                                                                                                                                                                               aTTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGAc      <  1:80584/249‑1 (MQ=255)

                                                                                                                                                                                                                                               aTTGCTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGAc      <  1:1423781/249‑1 (MQ=255)

                                                                                                                                                                                                                                                 tgctgcTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCt    >  2:175008/1‑249 (MQ=255)

                                                                                                                                                                                                                                                   cTGCTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCTGt  >  1:1374164/1‑249 (MQ=255)

GGACACATGTAGGGTGGCCGAAACAAAGTAATAGGACAACAACGCTCGACCGCGATTATTTTTGGAGAATCATGACCTCAGCATCTGCCCCAAGCTTTAACCCCGGCAAGGGTCCCGGCTCAGCAGTCGGAATTGCCCTTTTAGGATTCGGAACAGTCGGCACTGAGGTGATGCGTCTGATGACCGAGTACGGTGATGAACTTGCGCACCGCATTGGTGGCCCACTGGAGGTTCGTGGCATTGCTGTTTCTGATATCTCAAAGCCACGTGAAGGCGTTGCACCTGAGCTGCTCACTGAGGACGCTTTTGCACTCATCGAGCGCGAGGATGTTGACATCGTCGTTGAGGTTATCGGCGGCATTGAGTACCCACGTGAGGTAGTTCTCGCAGCTCTGAAGGCCGGCAAGTCTGTTGTTACCGCCAATAAGGCTCTTGTTGCAGCTCACTCTGCTGAGCTTGCTGATGCAGCGGAAGCCGCAAACGTTGACCTGT  >  CP025533/1244168‑1244659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`