Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 153,864 C→T S181S (TCC→TCT)  CYL77_00770 → permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP025533153,8640CT99.4% 675.2 / NA 167S181S (TCC→TCT) CYL77_00770permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (90/76);  minor base G (0/1);  total (90/77)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TCTCATTGACAAACAAATCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCCGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGTAGA  >  CP025533/153618‑154105
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
    aTTGACAAACAAATCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTTGCCTGAACGTGGTGTTCAAACCAAACTTCGGAATCGGCGAAAGCAAAGCCATGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACCCATCGCAACCTACGACGGCATCTTCGGACCCGGAACATGCATGATTCTCATCATGTCATTCACCACACTGCTCTCTAAAAATTTCGGTTCCTTTGCAGcc                                                                                                                                                                                                                                             >  2:521246/1‑249 (MQ=255)
       gACAAACAAATCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAACTTTCCTGTCCTCTGCAGCCATg                                                                                                                                                                                                                                          <  2:655057/249‑1 (MQ=255)
       gACAAACAAATCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATg                                                                                                                                                                                                                                          >  2:769064/1‑249 (MQ=255)
             caaaTCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCACTGCCCACCGGATGGAAACGCTGGGCCGACATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCGCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAg                                                                                                                                                                                                                                    >  2:1135897/1‑249 (MQ=255)
                aTCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTCCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTc                                                                                                                                                                                                                                 <  2:1216032/249‑1 (MQ=255)
                  cATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGt                                                                                                                                                                                                                               >  2:1067139/1‑249 (MQ=255)
                   aTGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTa                                                                                                                                                                                                                              >  2:683228/1‑249 (MQ=255)
                   aTGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTa                                                                                                                                                                                                                              >  2:942196/1‑249 (MQ=255)
                   aTGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTa                                                                                                                                                                                                                              <  1:278830/249‑1 (MQ=255)
                     gCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTaaa                                                                                                                                                                                                                            <  1:837931/249‑1 (MQ=255)
                       gACAGCTGATCATCGTGTTGTTGCTGTTCGTTGGCCTGTTCGTGTTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGCCTCATCGCAGCCTAGGACGGCATCTTCGGACCGGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAaca                                                                                                                                                                                                                          <  2:1016619/249‑1 (MQ=255)
                         ccGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACAcc                                                                                                                                                                                                                        <  1:10852/249‑1 (MQ=255)
                           gCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGc                                                                                                                                                                                                                      <  2:1494160/249‑1 (MQ=255)
                              gATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGcaac                                                                                                                                                                                                                   >  2:553513/1‑249 (MQ=255)
                               atcatcGGTTAGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGcaaca                                                                                                                                                                                                                  <  2:25304/249‑1 (MQ=255)
                                   tcGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGATCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAGTTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAAcc                                                                                                                                                                                                              <  1:1003381/249‑1 (MQ=255)
                                    cGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCt                                                                                                                                                                                                             <  2:238659/249‑1 (MQ=255)
                                    cGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCt                                                                                                                                                                                                             >  2:1077949/1‑249 (MQ=255)
                                     gtgtTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTg                                                                                                                                                                                                            <  2:927081/249‑1 (MQ=255)
                                      tgtTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTgg                                                                                                                                                                                                           >  2:1175662/1‑249 (MQ=255)
                                      tgtTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTgg                                                                                                                                                                                                           >  2:347191/1‑249 (MQ=255)
                                         tGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTg                                                                                                                                                                                                        >  2:1287450/1‑249 (MQ=255)
                                             gCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCt                                                                                                                                                                                                    <  1:175150/249‑1 (MQ=255)
                                               tGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTaa                                                                                                                                                                                                  >  1:1398057/1‑249 (MQ=255)
                                               tGGTCGTTGGCCTGATCGTGGTGTTCAAAACAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCCGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATTGCGGTCACCGCACTGCTCTCCCAAAATTTTCTCTCCTCTGCCGCCATGGCGAAGGTCGTAAACACAGCCACAAACCTGGGTCCGCCaa                                                                                                                                                                                                  >  2:1266859/1‑249 (MQ=255)
                                                ggTCGTTGGCCTGCTCGTGGTGTTCAAAGCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCTTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCATCAAACCTGGGTGCGCTAAt                                                                                                                                                                                                 <  2:1258719/249‑1 (MQ=255)
                                                  tCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAAtag                                                                                                                                                                                               >  2:513851/1‑247 (MQ=255)
                                                          cTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATtcatc                                                                                                                                                                                       >  2:42848/1‑249 (MQ=255)
                                                                 tggtgTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATcggcg                                                                                                                                                                                >  2:970858/1‑249 (MQ=255)
                                                                          aaccaaacTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGAGGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACAtgt                                                                                                                                                                       <  2:1348789/249‑1 (MQ=255)
                                                                             caaacTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATgtggt                                                                                                                                                                    <  1:1423363/249‑1 (MQ=255)
                                                                             caaacTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATgtggt                                                                                                                                                                    >  1:32585/1‑249 (MQ=255)
                                                                                 cTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGAc                                                                                                                                                                >  1:729183/1‑249 (MQ=255)
                                                                                 cTTCGGAACCGGAGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGAc                                                                                                                                                                >  2:1388710/1‑249 (MQ=255)
                                                                                       aaCCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAgg                                                                                                                                                          <  2:218548/249‑1 (MQ=255)
                                                                                        aCCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAggg                                                                                                                                                         >  2:157507/1‑248 (MQ=255)
                                                                                          cGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACt                                                                                                                                                       >  2:427043/1‑249 (MQ=255)
                                                                                          cGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACt                                                                                                                                                       <  2:1225759/249‑1 (MQ=255)
                                                                                           ggCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTc                                                                                                                                                      >  1:1069540/1‑249 (MQ=255)
                                                                                           ggCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTc                                                                                                                                                      >  1:1277585/1‑249 (MQ=255)
                                                                                             cGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCACCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGt                                                                                                                                                    <  1:794668/249‑1 (MQ=255)
                                                                                              gAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTg                                                                                                                                                   >  1:533941/1‑249 (MQ=255)
                                                                                                  gcaaagcCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTgg                                                                                                                                               <  1:1136504/249‑1 (MQ=255)
                                                                                                     aagcCCTGCCCAACGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAg                                                                                                                                            <  2:333070/249‑1 (MQ=255)
                                                                                                                  cGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGc                                                                                                                               >  2:465773/1‑249 (MQ=255)
                                                                                                                   ggATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGcn                                                                                                                              >  2:459788/1‑248 (MQ=255)
                                                                                                                    gATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAg                                                                                                                             <  1:1291102/249‑1 (MQ=255)
                                                                                                                                 ggCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCgg                                                                                                                >  1:472864/1‑249 (MQ=255)
                                                                                                                                  gccgccATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGAGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACAGCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGt                                                                                                               >  2:611632/1‑249 (MQ=255)
                                                                                                                                          cGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAAc                                                                                                       >  2:636127/1‑249 (MQ=255)
                                                                                                                                              gCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTg                                                                                                   <  1:1142588/249‑1 (MQ=255)
                                                                                                                                               cAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGc                                                                                                  <  1:942196/249‑1 (MQ=255)
                                                                                                                                                  tCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTtg                                                                                               <  2:1315360/249‑1 (MQ=255)
                                                                                                                                                    ggACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTtggt                                                                                             <  2:1223371/249‑1 (MQ=255)
                                                                                                                                                    ggACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTtggt                                                                                             >  1:64756/1‑249 (MQ=255)
                                                                                                                                                            cGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTAc                                                                                     >  1:291086/1‑249 (MQ=255)
                                                                                                                                                             gCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTAcc                                                                                    >  2:188780/1‑249 (MQ=255)
                                                                                                                                                               aGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAg                                                                                  >  2:88988/1‑249 (MQ=255)
                                                                                                                                                                gCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAgg                                                                                 <  2:287539/249‑1 (MQ=255)
                                                                                                                                                                gCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAgg                                                                                 <  2:1392775/249‑1 (MQ=255)
                                                                                                                                                                 ccTACGCCGGCATCTTCGGACTCGGAACCGGCATGTTCCGCATCATGGCTTTCACCTCACTGCTCTCCCAAATTTTCCTGTCCTCTGCAGCCATGCCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAGTTGTATTCATCATCGGCGGCCACATGTGGTTGGCCCGAGGCCTCGTGCTGGCAGTCGCCAATGTCTGAGGCCCACACCGCGGTTCCCCACCGGTGCTGCGGGGCGGtcacgggc                                                                                >  2:726398/1‑242 (MQ=255)
                                                                                                                                                                 ccTACGCCGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGc                                                                                <  2:309989/249‑1 (MQ=255)
                                                                                                                                                                  cTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCt                                                                               <  1:85957/249‑1 (MQ=255)
                                                                                                                                                                   tACGACGGCATCTTCGGACCCGGAACCGGCATGTACCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCCGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGCTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTa                                                                              >  1:792330/1‑249 (MQ=255)
                                                                                                                                                                          gCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGAt                                                                       <  1:42848/249‑1 (MQ=255)
                                                                                                                                                                            aTCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATAc                                                                     <  1:449618/249‑1 (MQ=255)
                                                                                                                                                                              nntCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGc                                                                   <  1:683228/247‑1 (MQ=255)
                                                                                                                                                                                    aCCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGAactact                                                             <  1:857610/249‑1 (MQ=255)
                                                                                                                                                                                      ccGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTaa                                                           >  2:792549/1‑249 (MQ=255)
                                                                                                                                                                                       cGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAAc                                                          <  1:370199/249‑1 (MQ=255)
                                                                                                                                                                                         gAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAAccc                                                        <  1:740233/249‑1 (MQ=255)
                                                                                                                                                                                           aCCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTg                                                      <  2:584790/249‑1 (MQ=255)
                                                                                                                                                                                             cGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGt                                                    <  2:143721/249‑1 (MQ=255)
                                                                                                                                                                                             cGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGt                                                    >  1:1298764/1‑249 (MQ=255)
                                                                                                                                                                                             cGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGt                                                    >  1:371491/1‑249 (MQ=255)
                                                                                                                                                                                              ggCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGtt                                                   <  2:180229/249‑1 (MQ=255)
                                                                                                                                                                                                    ttCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATAGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCAGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTgtcgtc                                             >  2:663464/1‑249 (MQ=255)
                                                                                                                                                                                                     tCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCa                                            >  1:1078273/1‑249 (MQ=255)
                                                                                                                                                                                                      ccTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTTACCCTGGTTGTCGTCAt                                           >  1:458210/1‑249 (MQ=255)
                                                                                                                                                                                                      ccTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCAt                                           <  1:1407468/249‑1 (MQ=255)
                                                                                                                                                                                                      ccTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCAt                                           <  1:51597/249‑1 (MQ=255)
                                                                                                                                                                                                          atcatGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGc                                       >  1:174615/1‑249 (MQ=255)
                                                                                                                                                                                                              tGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCt                                   >  1:1147673/1‑249 (MQ=255)
                                                                                                                                                                                                                   ttCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTc                              <  2:1422843/249‑1 (MQ=255)
                                                                                                                                                                                                                               ctctCCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGcaacaa                  <  2:483466/249‑1 (MQ=255)
                                                                                                                                                                                                                                tctcCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACaaa                 >  1:985202/1‑249 (MQ=255)
                                                                                                                                                                                                                                  tcCCAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATc               >  2:1156685/1‑249 (MQ=255)
                                                                                                                                                                                                                                    ccAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCa             <  1:633822/249‑1 (MQ=255)
                                                                                                                                                                                                                                    ccAAAATTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCa             >  1:204061/1‑249 (MQ=255)
                                                                                                                                                                                                                                      aaaaTTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTCACCCTAGTTGTCGTCATGACCGGCTACCTCACCTGGCAACAAATCCAAg           >  2:440220/1‑249 (MQ=255)
                                                                                                                                                                                                                                      aaaaTTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAg           >  1:1046927/1‑249 (MQ=255)
                                                                                                                                                                                                                                      aaaaTTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAg           <  1:553513/249‑1 (MQ=255)
                                                                                                                                                                                                                                       aaaTTTCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAgg          <  1:725257/249‑1 (MQ=255)
                                                                                                                                                                                                                                        attttCCTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAACTGTATTCCTCATCGGCGGCCACATGTGGTGGACCCTAGGCCTCGCGCTGGCAGTCGCCACTGTCGCAGGCGCCCAACTCGGTGCCCGAACCGTGCTTGGTGGCGGTACCAGGCTGACTAGATACGCACTACTATCCCTGGTTGTCGTCATGCGCGTCTACCTCACCTGGCACCAAATCCAAGGa         <  2:1301676/247‑1 (MQ=255)
                                                                                                                                                                                                                                         aTTTCCTGTCCTGTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAATCCTGGTTGCGCTAATTGTATTCATCATCGGTGTCCACATGTGGTGGACCCTAGGACACGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCTGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCGCACCTGGCAACAAATCCattgaa        <  2:1233760/249‑6 (MQ=255)
                                                                                                                                                                                                                                             ccTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGtn    >  2:843604/1‑248 (MQ=255)
                                                                                                                                                                                                                                             ccTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGtn    >  1:911821/1‑248 (MQ=255)
                                                                                                                                                                                                                                              cggTCCTCGGCAGCCAAGGGGAAGGTCGTAAACACCGCAACAAACCTGTGTGCGCTAATCGTATTCAGCATCGGCGGCCACAGGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGTTGCGTGGTGGCGGTACCAGGCTAATTAGATTCGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGTAg   <  2:777382/247‑1 (MQ=255)
                                                                                                                                                                                                                                              cTGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGtnn   >  1:1361864/1‑247 (MQ=255)
                                                                                                                                                                                                                                               tGTCCTCTGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGtnnn  >  2:99902/1‑246 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
TCTCATTGACAAACAAATCATGCGACCGCTGATCATCGTGTTGATGCTGGTCGTTGGCCTGATCGTGGTGTTCAAACCAAACTTCGGAACCGGCGAAAGCAAAGCCCTGCCCACCGGATGGAAACGCTGGGCCGCCATCGTTGCAGTCGGACTCATCGCAGCCTACGACGGCATCTTCGGACCCGGAACCGGCATGTTCCTCATCATGGCGTTCACCGCACTGCTCTCCCAAAATTTCCTGTCCTCCGCAGCCATGGCGAAGGTCGTAAACACCGCAACAAACCTGGGTGCGCTAATTGTATTCATCATCGGCGGCCACATGTGGTGGACCCTAGGACTCGTGCTGGCAGTCGCCAATGTCGCAGGCGCACAACTCGGTGCCCGAACGGTGCTTGGTGGCGGTACCAGGCTAATTAGATACGCACTACTAACCCTGGTTGTCGTCATGAGCGTCTACCTCACCTGGCAACAAATCCAAGGAATGTAGA  >  CP025533/153618‑154105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑