BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP025533	1,611,226	(C)_7→8	noncoding (80/85 nt)	CYL77_07745 →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	1,611,219	1	.	C	90.2%	357.9 / 30.0	112	noncoding (73/85 nt)	CYL77_07745	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (1/10); new base C (54/47); total (55/57)
Fisher's exact test for biased strand distribution p-value = 8.28e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTT  >  CP025533/1610971‑1611460

aTCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑t                                                                                                                                                                                                                                                       <  2:440054/249‑1 (MQ=255)

       ggAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑c‑ccc‑cc‑c                                                                                                                                                                                                                                            >  1:132327/1‑249 (MQ=255)

         aaTTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑c‑ccc‑cc‑ccg                                                                                                                                                                                                                                          <  2:1115134/249‑3 (MQ=255)

           ttCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCC‑CC‑Ccggg                                                                                                                                                                                                                                        <  1:415387/249‑2 (MQ=255)

           ttCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCC‑CC‑Ccggg                                                                                                                                                                                                                                        <  2:460083/249‑2 (MQ=255)

            tCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCC‑CC‑Ccgggc                                                                                                                                                                                                                                       <  1:1253862/249‑3 (MQ=255)

                 tGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGcacaaa                                                                                                                                                                                                                                   >  2:1203976/1‑247 (MQ=255)

                 tGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACt                                                                                                                                                                                                                                   <  1:912067/249‑1 (MQ=255)

                     aTGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTgtgt                                                                                                                                                                                                                               <  2:1040715/249‑1 (MQ=255)

                     aTGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTgtgt                                                                                                                                                                                                                               <  2:264126/249‑1 (MQ=255)

                      tGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGcacaaattagt                                                                                                                                                                                                                              >  2:963436/1‑242 (MQ=255)

                       ggCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTttgttg                                                                                                                                                                                                                             >  2:1004882/1‑249 (MQ=255)

                       ggCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTg                                                                                                                                                                                                                             <  2:363562/249‑1 (MQ=255)

                         cGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTgag                                                                                                                                                                                                                           <  1:392507/249‑1 (MQ=255)

                              acacTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTTTGTTGAGACAGt                                                                                                                                                                                                                      >  2:166972/1‑249 (MQ=255)

                                    ccGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTaa                                                                                                                                                                                                                <  2:1205774/249‑1 (MQ=255)

                                     cGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTann                                                                                                                                                                                                               >  2:417958/1‑247 (MQ=255)

                                           nttATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTg                                                                                                                                                                                                         <  2:1035757/248‑1 (MQ=255)

                                             tataCGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGGTGAGACAGTATTTAAGACTCTGaa                                                                                                                                                                                                       >  2:1072633/1‑249 (MQ=255)

                                               taCGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACt                                                                                                                                                                                                     <  1:157564/249‑1 (MQ=255)

                                                  ggACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTa                                                                                                                                                                                                  <  1:391127/249‑1 (MQ=255)

                                                   gACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTATTTAAATCATCTTTTAGACTCTTAACtattt                                                                                                                                                                                                 >  2:692317/1‑245 (MQ=25)

                                                      aGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCa                                                                                                                                                                                              <  2:372489/249‑1 (MQ=255)

                                                      aGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAAATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGGGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTATGACTATGAACTTTATTCa                                                                                                                                                                                              >  1:591611/1‑249 (MQ=255)

                                                            tACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACAGTAGTTAAACAGTATTTAACAATCTGAACTTTATTCAAGtgcn                                                                                                                                                                                        >  2:1285024/1‑246 (MQ=37)

                                                                aaGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAAATTTAGTAAAGTGCAGAGt                                                                                                                                                                                    >  2:1270176/1‑249 (MQ=255)

                                                                  gCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCt                                                                                                                                                                                  >  1:1181473/1‑249 (MQ=255)

                                                                   cTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCtt                                                                                                                                                                                 <  1:683996/249‑1 (MQ=255)

                                                                      tAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTTTATTTAGATCGCATTGTAGACTCTGAATTTTTTTACAGTACAGTGTCTTaat                                                                                                                                                                              >  2:142634/1‑247 (MQ=25)

                                                                       aaGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcct                                                                                                                                                                             <  2:1352607/249‑1 (MQ=255)

                                                                       aaGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcct                                                                                                                                                                             <  1:75538/249‑1 (MQ=255)

                                                                        aGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAcctt                                                                                                                                                                            <  1:539284/249‑1 (MQ=255)

                                                                              aCTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgcat                                                                                                                                                                      >  2:577647/1‑247 (MQ=255)

                                                                                 ttGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGATAGTATTTAAGACTCTTAACTTTATTAAAGTCCAGAGTTTTAtcttatgccttat                                                                                                                                                                   >  2:1168750/1‑249 (MQ=255)

                                                                                  tGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTAccttatgccttatg                                                                                                                                                                  >  1:599662/1‑249 (MQ=255)

                                                                                   gTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGt                                                                                                                                                                 <  1:1071670/249‑1 (MQ=255)

                                                                                    tCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGtt                                                                                                                                                                >  1:555276/1‑249 (MQ=255)

                                                                                      tttttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGTGTCTTACCTTATGCCTTATGtttt                                                                                                                                                              >  1:122731/1‑249 (MQ=255)

                                                                                       ttttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTa                                                                                                                                                             <  2:736466/249‑1 (MQ=255)

                                                                                       ttttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTa                                                                                                                                                             <  2:1189921/249‑1 (MQ=255)

                                                                                        tttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTAc                                                                                                                                                            <  1:311552/249‑1 (MQ=255)

                                                                                        tttAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTAc                                                                                                                                                            <  1:641361/249‑1 (MQ=255)

                                                                                          tAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACGCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACta                                                                                                                                                          >  2:832320/1‑248 (MQ=255)

                                                                                            gCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTTAGACAGTACTTAAGAATCTGAACTCTATTCAAGTCCAAAGTCTTACCTTATGCATTGTTGTTTACTcac                                                                                                                                                        >  2:476908/1‑249 (MQ=37)

                                                                                              tAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGTGCACACTGTGTTGAGACAGTATTTAAGACTATGAACTCTATTCAAGTCGAGAGTCTTACCTTATTACTTATGtttttataatac                                                                                                                                                      >  2:1228815/1‑241 (MQ=37)

                                                                                               aaTGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTcacaca                                                                                                                                                     >  1:737737/1‑249 (MQ=255)

                                                                                                aTGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCacacac                                                                                                                                                    <  2:254527/249‑1 (MQ=255)

                                                                                                   aCCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACAcccn                                                                                                                                                 >  1:723623/1‑248 (MQ=255)

                                                                                                    ccTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGGTGAAACAGTCTTTAAGAATCTTAACTCTTTTCAAGTCGGGAGTCTTACCTTATGCCTTATGTTTTACTCAAACACCCAg                                                                                                                                                >  2:373778/1‑249 (MQ=37)

                                                                                                      ngCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGcc                                                                                                                                              <  1:606032/248‑1 (MQ=255)

                                                                                                         aGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCgg                                                                                                                                           <  2:954772/249‑1 (MQ=255)

                                                                                                                 gtTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGa                                                                                                                                   <  2:1171645/249‑1 (MQ=255)

                                                                                                                    cAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATg                                                                                                                                >  1:1283320/1‑249 (MQ=255)

                                                                                                                     aaGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATgg                                                                                                                               <  1:831707/249‑1 (MQ=255)

                                                                                                                      aGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGc                                                                                                                              <  2:307704/249‑1 (MQ=255)

                                                                                                                      aGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTAATCACACATCCAACACGGGTAGCAGAATAGc                                                                                                                              >  2:467295/1‑249 (MQ=37)

                                                                                                                            gATTTGCGCTATTGTTTATCTCGTGCCAAGGGTAATACGCCGTAAACGTCCAGCCCTGGTGCCGGAGTGACAGACGCGCTAGCTTGAGGGGTTAGTGTCCTATTAACGGACGTGGGGGTACAAG‑C‑C‑CCC‑CCTCGGGGACACTGTGTTAAGACAGTATTTTAGACTCTGAAGTTTATTCAAGTCCAGAGTCTTACCTTCTGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGAcgc                                                                                                                        <  2:127889/249‑1 (MQ=255)

                                                                                                                                cGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTTAGACAGTATTTAAGACTCTTAACTTTATTCAAGTCCAGAGTCTTACTTTATGCCTTATTGTTTACTCACACACCCAGTCCGGGTGCCGGAATGGCAGACGCGCtt                                                                                                                    >  2:724201/1‑248 (MQ=37)

                                                                                                                                  ggCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGc                                                                                                                  >  1:647004/1‑249 (MQ=255)

                                                                                                                                   gCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCt                                                                                                                 >  2:245794/1‑249 (MQ=255)

                                                                                                                                      aTTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGa                                                                                                              <  1:1156229/249‑1 (MQ=255)

                                                                                                                                         gtctATCTCGTGCCACGGGAAATAAGTCCTAACCAACCCGGCCGGGTGGCGGAATGGCAGACACGCTAGCTTGAGGTGCTAGTGCCCTATTAACTGACGTGGGGGTTAAAG‑TCC‑CCC‑CC‑CGTGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGt                                                                                                           <  2:267730/246‑1 (MQ=255)

                                                                                                                                         gTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGt                                                                                                           <  1:182906/249‑1 (MQ=255)

                                                                                                                                                ncGTGCCAAGGGTAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAgtg                                                                                                    <  1:759950/248‑1 (MQ=255)

                                                                                                                                                  gTGGCAAAGGCAATGAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGAGGCGCTAGCTTGAGGGGCTTGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCCTCC‑CGGGTACAGTGTGTTGAGACAGTATTTAAGACTCTGGACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTc                                                                                                  <  2:187024/249‑1 (MQ=38)

                                                                                                                                                    gCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTCTTTAAGACGCTGAACTTTATTCAAGTGCAGAGTCTTACCTTATGTCTTATGTTTTACTCACACATCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGCTTCGAGAGTCCt                                                                                                >  2:272835/1‑249 (MQ=37)

                                                                                                                                                     ccAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTa                                                                                               >  1:1162449/1‑249 (MQ=255)

                                                                                                                                                       aaGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTTAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCACAGTCTTACCTTATCCCTTATGTTTTACTAACACACCCAACCCGGGTGGCGTAATGGCAGTCTCGCTCGCTTTAGGTTCTCGTATCCTAtt                                                                                             >  2:476860/1‑249 (MQ=25)

                                                                                                                                                            cAATCAGCCCTAAACACTCAGCCCGCGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTCGTGTCCTATTATCGGTCGTGGGCGTTCATGCG‑C‑TCT‑CC‑TGTGCTTAGTGTGTTGAGACCGGATTTGTGTCCCTGGACTGAATCGAAGTCTAGATTCTTTCGTGACGCCGTATGTTTTCCTTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACgg                                                                                        <  2:599662/249‑1 (MQ=16)

                                                                                                                                                            cAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACgg                                                                                        <  2:1162449/249‑1 (MQ=255)

                                                                                                                                                                   cccTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTTAGACAGTATTTTAGAATCTTAACTTTATTCAAGTCCGGAGTTTTGTCTTTTGTCTTGTGGTTTAATCACACACTCTGTCCGGGTGGCGGTATGGCAGTCGCGATAGCTTGATTTTCTAGTGTCCTGTTCACGTActtcgt                                                                                 >  2:1036748/1‑244 (MQ=18)

                                                                                                                                                                      tAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAATATTTAAGACTCTTACCTTTATTCCAGTCTGAAGTTTTGCCTTATGCCTTACTGCTTACTAATCCACCCAATCCTGCGGCCGCAATGGCAGACTTGCTTGCTTGGGTTTCGAGTATCCAATTAActtacttcttgtt                                                                              >  2:1098701/1‑237 (MQ=12)

                                                                                                                                                                       aaaCACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAATGGACGTGGGGGtt                                                                             <  1:614608/249‑1 (MQ=255)

                                                                                                                                                                           acCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACTGACGTGGGGGTTCAAg                                                                         >  1:8485/1‑249 (MQ=255)

                                                                                                                                                                                  ccGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTTAGACAGTATTTTAGACTCTGAACTTTATTCAAGGCCAGAGTCTTACCTTATCCCTTATGTTTTACTCACACATCCAGCCCGGGGGGCGGAATGGCTGACTTGCTTGCTTGAGGTGCTAGTGTCCTATTAACGTACTTTGGGTTTCAAGTcccccc                                                                  >  2:428566/1‑249 (MQ=25)

                                                                                                                                                                                   cGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACCGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTACGTTTTACTCACACACCCAGCCCGGCTGCCGGAATGGCAGACGCGCCAGCTTTAGGTGCTAGTGTCCTGTTAACTGACTTCTGGGTTCACGcccccccc                                                                 >  2:620437/1‑249 (MQ=37)

                                                                                                                                                                                       ttgCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCggg                                                             <  1:1213605/247‑1 (MQ=255)

                                                                                                                                                                                        ggCGTAGTGGTAGCCGCAGTAGGTTGGCGTGCTTGTATGCTATTAACGGACGTGGGGGTTCATG‑T‑C‑CCCTTG‑CCGGCACACTGTGTTGAGAGAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTTACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGc                                                            <  2:625225/249‑1 (MQ=25)

                                                                                                                                                                                        ggCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTTTGTTTAGACAGTACTTAAGACTCTGAACTTTTTTCAAGTCCAGAGTCTTACTTTATGCCTTATGTTTCACTCACACACCCAGTCCTGCGGGCAGTATGGCTGATGTGCTAGCTTTATGTTCTGATATCCTATTATCGTATGTCGTGGTTGACGTCTACCCCCggtc                                                            >  2:518127/1‑247 (MQ=16)

                                                                                                                                                                                             aaTGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACaaa                                                       <  1:606543/249‑1 (MQ=255)

                                                                                                                                                                                                      cgcgCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTaaa                                              <  1:669585/249‑1 (MQ=255)

                                                                                                                                                                                                        cgcTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATc                                            >  1:772962/1‑249 (MQ=255)

                                                                                                                                                                                                             gCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCt                                       >  1:41343/1‑249 (MQ=255)

                                                                                                                                                                                                               ttGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACAGTGTTGAGACAGTATTTAACACTCTGACCTTTATTCAAGTCCAGAGTCTTACCTTATGCATTATGTTTTACTAATACACCCAGCCCGGGTGGCAAAATGGCAGACGCGCTAGCTTGAGTTGCTATTGTCCTGTTAACGCACGTGGTGATTCAAGTCCCCCCCCGGGCACATATTTGTTATAACACACttt                                     >  2:482141/1‑247 (MQ=37)

                                                                                                                                                                                                                      gCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCCACC‑CGGGCACACAATGGTGAGATCCCACTTAAGACTATGAACTCTATTCAAGGCACAAGTCATGCCTTATATATCATAATTTAATAATACACCCAGCACAGCGAGCGGAATGCCAGATGCGCCTGCTTAGGGTGATAGAGTCCTATCAACTGATGTGGTGGTTCAAGTCTCCCATTGGGCACATATTACTTTAAGCACCCGCTACAAGAt                              >  2:848982/1‑249 (MQ=255)

                                                                                                                                                                                                                         aGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTTAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTTAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTACTTTAATCACACTGTACAAGATTAc                           >  2:1046756/1‑249 (MQ=255)

                                                                                                                                                                                                                         aGTGTCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTAc                           <  1:1177804/249‑1 (MQ=255)

                                                                                                                                                                                                                             tCCTATTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTActtct                       <  1:1291599/249‑1 (MQ=255)

                                                                                                                                                                                                                                 aTTAACTGACGTGGGTGTTCATG‑T‑CACTC‑TC‑TGGTCTCAGTGTGTGGCGACAGTATTTGAGTTCCAGGACTGTATTCAGCTCCAGAGTCTTACGTGATGACTGATATTTTCCTTACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTa                   <  2:258588/249‑1 (MQ=18)

                                                                                                                                                                                                                                 aTTAACGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTa                   <  1:373778/249‑1 (MQ=255)

                                                                                                                                                                                                                                    aaCGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGTTGCTAGTGTCCTATTAACGTACGTGGTGGTTTAAGTCCCCCCCCGGGCCCAAATTAGTTAAATCCCACTGTACAATATTACTTCTTGTAAAg                >  2:1232380/1‑249 (MQ=38)

                                                                                                                                                                                                                                    aaCGGACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAg                >  1:355823/1‑249 (MQ=255)

                                                                                                                                                                                                                                       ggACGTGGGGGTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGt             <  1:959967/249‑1 (MQ=255)

                                                                                                                                                                                                                                            tGGGGGATCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGGGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCCATTAACGGACGTCGGGCTTCAAGTCCCCCCCCGGGCACAAATTACTTAAATCACCCTGTACAAGTTTTCTTCCTGTACAGGAGGAttt        >  2:943529/1‑249 (MQ=37)

                                                                                                                                                                                                                                             gggggTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtttt       <  1:832320/249‑1 (MQ=255)

                                                                                                                                                                                                                                             gggcgTTCAAG‑TCC‑CCC‑CC‑CGGGCATACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGAtttt       <  1:367377/249‑1 (MQ=255)

                                                                                                                                                                                                                                               gggTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCt     <  2:1181473/249‑1 (MQ=255)

                                                                                                                                                                                                                                                ggTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTg    <  1:1129825/249‑1 (MQ=255)

                                                                                                                                                                                                                                                ggTTCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTg    >  1:945348/1‑249 (MQ=255)

                                                                                                                                                                                                                                                  ttCAAG‑TCC‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGtt  <  1:1004882/249‑1 (MQ=255)

ATCCTGGGGAATTCATTTGTCATGGCGAAAACACTACCGCAGCTTTATACGGACAGTACTTACAAAGCTGTAAGTACAACTTTGTCTTTTTAGCTAATGACCTGCAGATTTGTGTTCAAGGAAAGATTCGGGCTATTGTTTATCTCGTGCCAAGGGCAATAAGCCCTAAACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAG‑T‑C‑CCC‑CC‑CGGGCACACTGTGTTGAGACAGTATTTAAGACTCTGAACTTTATTCAAGTCCAGAGTCTTACCTTATGCCTTATGTTTTACTCACACACCCAGCCCGGGTGGCGGAATGGCAGACGCGCTAGCTTGAGGTGCTAGTGTCCTATTAACGGACGTGGGGGTTCAAGTCCCCCCCCGGGCACAAATTAGTTAAATCACCCTGTACAAGATTACTTCTTGTACAGGGTGATTTTCTGTT  >  CP025533/1610971‑1611460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`