Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 2,565,278 +A pseudogene (61/1349 nt) CYL77_12135 ← trigger factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0255332,565,2751.A98.2% 449.8 / ‑0.4 111pseudogene (64/1349 nt)CYL77_12135trigger factor
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base A (53/56);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 119 total aligned reads displayed.

ACGAGCTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑AAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGTTCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTG  >  CP025533/2565029‑2565517
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
aCGAGCTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAATCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑aa                                                                                                                                                                                                                                                  >  1:981333/1‑249 (MQ=255)
 cGAGCTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCTGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑aaa                                                                                                                                                                                                                                                 >  1:476607/1‑249 (MQ=255)
  gAGCTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑aaaa                                                                                                                                                                                                                                                >  2:1212306/1‑248 (MQ=255)
     cTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑aaaatgg                                                                                                                                                                                                                                             >  2:1005946/1‑245 (MQ=255)
     cTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑aaaatgg                                                                                                                                                                                                                                             >  1:917050/1‑245 (MQ=255)
       cGTTGTCTTCGATCTTGGTTACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGaa                                                                                                                                                                                                                                            <  2:1132609/249‑1 (MQ=255)
       cGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGaa                                                                                                                                                                                                                                            >  1:971234/1‑249 (MQ=255)
        gTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAAc                                                                                                                                                                                                                                           <  1:1050449/249‑1 (MQ=255)
               tCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACg                                                                                                                                                                                                                                    <  1:965458/249‑1 (MQ=255)
                cGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACgg                                                                                                                                                                                                                                   <  2:260655/249‑1 (MQ=255)
                    cTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGAt                                                                                                                                                                                                                               >  1:1047769/1‑249 (MQ=255)
                     ttGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATc                                                                                                                                                                                                                              >  2:604282/1‑249 (MQ=255)
                      tGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCt                                                                                                                                                                                                                             <  2:635839/249‑1 (MQ=255)
                      tGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCt                                                                                                                                                                                                                             <  1:359918/249‑1 (MQ=255)
                      tGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCt                                                                                                                                                                                                                             >  1:903182/1‑249 (MQ=255)
                           aCCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGaa                                                                                                                                                                                                                        <  2:31707/249‑1 (MQ=255)
                                naCGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGgt                                                                                                                                                                                                                   <  2:1189367/248‑1 (MQ=255)
                                     tAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGc                                                                                                                                                                                                              >  2:808618/1‑249 (MQ=255)
                                           ggCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCt                                                                                                                                                                                                        <  1:1324890/249‑1 (MQ=255)
                                               gATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTtctc                                                                                                                                                                                                    <  2:375468/249‑1 (MQ=255)
                                                      ntGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGaa                                                                                                                                                                                             <  1:831618/248‑1 (MQ=255)
                                                           gCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCtt                                                                                                                                                                                        >  2:350143/1‑249 (MQ=255)
                                                           gCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCtt                                                                                                                                                                                        >  2:902752/1‑249 (MQ=255)
                                                             ccTCAGCTTCGATTGCGTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCa                                                                                                                                                                                      >  1:521976/1‑249 (MQ=255)
                                                             ccTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCa                                                                                                                                                                                      >  1:98499/1‑249 (MQ=255)
                                                             ccTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCa                                                                                                                                                                                      >  2:1130649/1‑249 (MQ=255)
                                                             ccTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCa                                                                                                                                                                                      <  1:1141225/249‑1 (MQ=255)
                                                              cTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCAc                                                                                                                                                                                     >  2:916427/1‑249 (MQ=255)
                                                              cTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCAc                                                                                                                                                                                     >  1:786662/1‑249 (MQ=255)
                                                                      cGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCGTCACGAGTAAAt                                                                                                                                                                             >  2:402225/1‑249 (MQ=255)
                                                                              ngtgCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGga                                                                                                                                                                     <  2:1107779/248‑1 (MQ=255)
                                                                                    tAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCg                                                                                                                                                               <  2:903182/249‑1 (MQ=255)
                                                                                    tAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCg                                                                                                                                                               >  1:80214/1‑249 (MQ=255)
                                                                                        ggTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCgggaa                                                                                                                                                           >  2:511694/1‑249 (MQ=255)
                                                                                           tAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCgggaaagg                                                                                                                                                        <  1:159762/249‑1 (MQ=255)
                                                                                           tAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCgggaaagg                                                                                                                                                        <  2:242483/249‑1 (MQ=255)
                                                                                            aGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCgggaaaggg                                                                                                                                                       >  1:815058/1‑249 (MQ=255)
                                                                                              gAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCgggaaagggaa                                                                                                                                                     <  2:58104/249‑1 (MQ=255)
                                                                                                  cATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGtt                                                                                                                                                 <  2:851966/249‑1 (MQ=255)
                                                                                                    tGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTc                                                                                                                                               <  1:399783/249‑1 (MQ=255)
                                                                                                    tGTCGTTCACAACCTGCTCCAGAACCGCACAGCGGCCGAAGCGTGCGTAGATAAGCTGACGCAGTGCCTTGCCCTTACGGAAATCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTc                                                                                                                                               <  2:36384/249‑1 (MQ=255)
                                                                                                     gTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCa                                                                                                                                              <  2:1139622/249‑1 (MQ=255)
                                                                                                        gTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGat                                                                                                                                           >  1:340459/1‑249 (MQ=255)
                                                                                                            aCAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTa                                                                                                                                       <  2:467127/249‑1 (MQ=255)
                                                                                                              aaCCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACa                                                                                                                                     >  2:833655/1‑249 (MQ=255)
                                                                                                                   gCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAAc                                                                                                                                <  2:645091/249‑1 (MQ=255)
                                                                                                                   gCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAAc                                                                                                                                >  1:20955/1‑249 (MQ=255)
                                                                                                                   gCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAAc                                                                                                                                <  2:940835/249‑1 (MQ=255)
                                                                                                                      ccAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAg                                                                                                                             <  1:605732/249‑1 (MQ=255)
                                                                                                                                 ccacGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCcg                                                                                                                  <  2:1224245/249‑1 (MQ=255)
                                                                                                                                   acGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCcgac                                                                                                                <  1:773070/249‑1 (MQ=255)
                                                                                                                                    cGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCcgacg                                                                                                               <  1:902752/249‑1 (MQ=255)
                                                                                                                                         gAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGTAAACCAGCGATAGGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGATTCAGTTCAGAAAATGGAACCTCAGCGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTTGCCAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTaa                                                                                                          <  1:580484/249‑1 (MQ=255)
                                                                                                                                          aaGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAAt                                                                                                         <  2:983149/249‑1 (MQ=255)
                                                                                                                                             cgtgcgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCg                                                                                                      >  2:436757/1‑249 (MQ=255)
                                                                                                                                              gtgcgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGa                                                                                                     <  1:1005946/249‑1 (MQ=255)
                                                                                                                                              gtgcgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGa                                                                                                     >  2:1082767/1‑249 (MQ=255)
                                                                                                                                                gcgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGAtt                                                                                                   <  2:494173/249‑1 (MQ=255)
                                                                                                                                                gcgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGAtt                                                                                                   >  2:920618/1‑249 (MQ=255)
                                                                                                                                                 cgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGGTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGAttt                                                                                                  <  1:178940/249‑1 (MQ=255)
                                                                                                                                                 cgtCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGAttt                                                                                                  <  2:949397/249‑1 (MQ=255)
                                                                                                                                                           cTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGtcgt                                                                                        >  2:350843/1‑246 (MQ=255)
                                                                                                                                                              aCGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCg                                                                                    <  1:119384/249‑1 (MQ=255)
                                                                                                                                                              aCGCGGTGCCTTGCCCTTACGGAAACCAGGGATATGGACTTGCTGCGCTAGAGCGGCGTCTGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTCATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTGCAGATATTACAAAAACCAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCg                                                                                    <  1:588747/249‑1 (MQ=255)
                                                                                                                                                                gcgGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGaa                                                                                  >  1:962753/1‑249 (MQ=255)
                                                                                                                                                                 cgGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGaaa                                                                                 <  2:675462/249‑1 (MQ=255)
                                                                                                                                                                 cgGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGaaa                                                                                 <  2:395049/249‑1 (MQ=255)
                                                                                                                                                                  ggTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAAc                                                                                >  1:458051/1‑249 (MQ=255)
                                                                                                                                                                     gCCTTGCCCTTACGGAAACCAGGCATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTTATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGa                                                                             <  1:566798/249‑1 (MQ=255)
                                                                                                                                                                       cTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGc                                                                           <  1:681084/249‑1 (MQ=255)
                                                                                                                                                                        ntGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCTGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCt                                                                          <  2:1311076/248‑1 (MQ=255)
                                                                                                                                                                           cccTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGt                                                                       <  2:1130355/249‑1 (MQ=255)
                                                                                                                                                                             cTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTcc                                                                     <  1:1344715/249‑1 (MQ=255)
                                                                                                                                                                                 cGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCaa                                                                 <  1:185498/249‑1 (MQ=255)
                                                                                                                                                                                   gAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGt                                                               >  1:1034480/1‑249 (MQ=255)
                                                                                                                                                                                   gAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCATCAAGt                                                               >  2:78225/1‑249 (MQ=255)
                                                                                                                                                                                      aCCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCgg                                                            <  2:798399/249‑1 (MQ=255)
                                                                                                                                                                                         aGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGc                                                         >  2:75005/1‑249 (MQ=255)
                                                                                                                                                                                           ggATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGa                                                       >  2:1081998/1‑249 (MQ=255)
                                                                                                                                                                                             nnnnggACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACa                                                     <  1:482258/245‑1 (MQ=255)
                                                                                                                                                                                             nnnnggACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCAAAACCGAGCTCGTCCTCAAGTCGGGGCGACa                                                     <  2:1270762/245‑1 (MQ=255)
                                                                                                                                                                                                   aCTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGAttt                                               >  2:278819/1‑249 (MQ=255)
                                                                                                                                                                                                      tgctgcGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGaa                                            >  2:314889/1‑249 (MQ=255)
                                                                                                                                                                                                        ctgcGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAAcc                                          >  2:1294336/1‑249 (MQ=255)
                                                                                                                                                                                                         tgcGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCt                                         >  1:474701/1‑249 (MQ=255)
                                                                                                                                                                                                               agagCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGAcc                                   >  1:826448/1‑249 (MQ=255)
                                                                                                                                                                                                                  gcggagTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGAccccc                                <  2:1193326/249‑1 (MQ=255)
                                                                                                                                                                                                                   cggcgTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCt                               >  2:1052252/1‑249 (MQ=255)
                                                                                                                                                                                                                       gTATGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTc                           >  1:363223/1‑249 (MQ=255)
                                                                                                                                                                                                                         aTGCCTGGTCGATCTCTGGCTTCAGTTCAGAAAATGAAACCTCAACGGGGATCTTTGAACGGGTGTCGCTCAGCTTCTCTACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATCTCTCGCG‑TCGACCGAAACCGAGATCGTCCTCAAGTAGGGTCGACAGGATTTGAATCTGCGACCCCCTGCTccc                         >  2:1082904/1‑249 (MQ=255)
                                                                                                                                                                                                                             cTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAg                     >  2:505149/1‑249 (MQ=255)
                                                                                                                                                                                                                             cTGGTCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACAAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAg                     >  1:341430/1‑249 (MQ=255)
                                                                                                                                                                                                                                 tCGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAgg                 >  2:760243/1‑249 (MQ=255)
                                                                                                                                                                                                                                  cGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGt                >  2:456390/1‑249 (MQ=255)
                                                                                                                                                                                                                                  cGATCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGt                >  2:396296/1‑249 (MQ=255)
                                                                                                                                                                                                                                    aTCTCTGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGAG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGCGGACAGGATTTGAACCTGGGACCCCCTTCTCCCAAAGCAGGGgc              >  2:46318/1‑249 (MQ=255)
                                                                                                                                                                                                                                      ctctGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGACTAATGCGATTTCTCGCG‑TCGTCCAAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATCTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTgcgc            >  2:1175041/1‑249 (MQ=255)
                                                                                                                                                                                                                                       nctGGCTTCAGTTCAGAAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCt           <  2:1139715/248‑1 (MQ=255)
                                                                                                                                                                                                                                             ttcagttcagaAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAg     >  2:730743/1‑249 (MQ=255)
                                                                                                                                                                                                                                                agttcagaAAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCG‑TCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTg  >  1:623224/1‑249 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
ACGAGCTCGTTGTCTTCGATCTTGGTGACCTCTACGTTAGGCTGGCCGATTGCCTTGATGCCCTCAGCTTCGATTGCCTGTGCGTAGCGGTTAGGAAGCATGTCGTTGACAACCTGCTCCAGAACCGCACCACGGCCGAAGCGTGCGTCGATAAGCTGACGCGGTGCCTTGCCCTTACGGAAACCAGGGATCTGGACTTGCTGCGCTAGAGCGGCGTATGCCTGGTCGATCTCTGGCTTCAGTTCAG‑AAATGGAACCTCAACGGTGATCTTTGAACGGGTGTCGCTCAGCTTCTCGACAGAACTCTTCACGAGTAAATCTCCTGGATGATCGGGAAAGGGAAAGTTTCAGATATTACAAAAACAAGCCCGGTTTCCGACGATTAATGCGATTTCTCGCGTTCGTCCGAAACCGAGCTCGTCCTCAAGTCGGGGCGACAGGATTTGAACCTGCGACCCCCTGCTCCCAAAGCAGGTGCGCTACCAAGCTG  >  CP025533/2565029‑2565517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑