Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 1,324,992 C→T P290S (CCA→TCA)  CYL77_06355 → iron‑siderophore ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0255331,324,9920CT99.3% 550.8 / NA 137P290S (CCA→TCA) CYL77_06355iron‑siderophore ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (71/65);  total (72/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 137 total aligned reads displayed.

CTGAGAAGACCGCGGCTGCTGTATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTCCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTGGGAA  >  CP025533/1324747‑1325236
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
cTGAGAAGACCGCGGCTGCTGTATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCCGAGCAGGTTTCAt                                                                                                                                                                                                                                                   >  2:1130212/1‑249 (MQ=255)
           gcgGCTGCTGTATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTg                                                                                                                                                                                                                                        <  1:468259/249‑1 (MQ=255)
           gcgGCTGCTGTATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTg                                                                                                                                                                                                                                        <  2:133031/249‑1 (MQ=255)
               ctgctgTATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCg                                                                                                                                                                                                                                    <  1:563497/249‑1 (MQ=255)
                     tATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTc                                                                                                                                                                                                                              >  2:582834/1‑249 (MQ=255)
                     tATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTc                                                                                                                                                                                                                              >  1:739883/1‑249 (MQ=255)
                        cTGCAACGGCAACCAGCTGGGGTGCATACAGCAAGGGCTCCAACCGTGTAGTTACTTTGCTGGACCTGGGCTTCCAGGAAAACACTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGAAGAGGAGGTTTCATGGCAGATGTTGACCGCCGCTCGTg                                                                                                                                                                                                                           >  2:343485/1‑249 (MQ=255)
                          gCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGAc                                                                                                                                                                                                                         >  2:147759/1‑249 (MQ=255)
                            aaCGGCAACCAGCTGAGGTGAATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACgg                                                                                                                                                                                                                       <  1:712597/249‑1 (MQ=255)
                             aCGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGc                                                                                                                                                                                                                      >  1:1271663/1‑249 (MQ=255)
                                gCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGt                                                                                                                                                                                                                   <  1:745402/249‑1 (MQ=255)
                                  aaCCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTc                                                                                                                                                                                                                 >  1:1253340/1‑249 (MQ=255)
                                             gTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGc                                                                                                                                                                                                      >  2:1265746/1‑249 (MQ=255)
                                                  tACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGCATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCATTGTGATGCCCCGt                                                                                                                                                                                                 >  1:270987/1‑249 (MQ=255)
                                                   aCATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTg                                                                                                                                                                                                <  2:493482/249‑1 (MQ=255)
                                                    cATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTga                                                                                                                                                                                               <  1:974016/249‑1 (MQ=255)
                                                     aTCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTgag                                                                                                                                                                                              >  2:1030796/1‑249 (MQ=255)
                                                      tCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTgaga                                                                                                                                                                                             <  2:878754/249‑1 (MQ=255)
                                                       cAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGAt                                                                                                                                                                                            <  2:385211/249‑1 (MQ=255)
                                                       cAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGAt                                                                                                                                                                                            >  2:940908/1‑249 (MQ=255)
                                                             cTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCaa                                                                                                                                                                                      <  1:9696/249‑1 (MQ=255)
                                                              tCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAAt                                                                                                                                                                                     >  1:1042014/1‑249 (MQ=255)
                                                                cAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGc                                                                                                                                                                                   >  2:1043438/1‑249 (MQ=255)
                                                                cAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGc                                                                                                                                                                                   <  2:1286778/249‑1 (MQ=255)
                                                                  aCCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGt                                                                                                                                                                                 <  2:352915/249‑1 (MQ=255)
                                                                   ccGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGtt                                                                                                                                                                                >  1:33815/1‑249 (MQ=255)
                                                                     gtgtAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGtttt                                                                                                                                                                              <  1:1122008/249‑1 (MQ=255)
                                                                     gtgtAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAAACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGCCTCCTGGGGAAATGGCAGGGCAGGTTCCATGGCAGATGTTGCGCGCCACTCGTGACGGCCGTTCCTTTTTGATGCCCCGTCAGATTTCCAATGcttatt                                                                                                                                                                              >  1:586625/1‑244 (MQ=255)
                                                                      tgtAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTc                                                                                                                                                                             >  2:349766/1‑249 (MQ=255)
                                                                           aTACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTgg                                                                                                                                                                        <  2:603667/249‑1 (MQ=255)
                                                                           aTACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTgg                                                                                                                                                                        >  2:474343/1‑249 (MQ=255)
                                                                                  gCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTcccc                                                                                                                                                                 >  2:631157/1‑249 (MQ=255)
                                                                                   cTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCg                                                                                                                                                                >  1:356104/1‑249 (MQ=255)
                                                                                         cTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTcc                                                                                                                                                          >  1:1215178/1‑249 (MQ=255)
                                                                                            ggCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACt                                                                                                                                                       <  2:1205007/249‑1 (MQ=255)
                                                                                                        aaCCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGCGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTa                                                                                                                                           <  1:638345/249‑1 (MQ=255)
                                                                                                                     cTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGc                                                                                                                              >  1:420069/1‑249 (MQ=255)
                                                                                                                                ccTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTggag                                                                                                                   <  2:1001386/249‑1 (MQ=255)
                                                                                                                                 cTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTggagg                                                                                                                  >  1:107815/1‑249 (MQ=255)
                                                                                                                                   ggCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGgaggag                                                                                                                >  2:777577/1‑249 (MQ=255)
                                                                                                                                     cGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCa                                                                                                              >  2:1179217/1‑249 (MQ=255)
                                                                                                                                      gATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCAt                                                                                                             >  2:10405/1‑249 (MQ=255)
                                                                                                                                              tttCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAggggn                                                                                                     >  2:405701/1‑248 (MQ=255)
                                                                                                                                              tttCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATCGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGa                                                                                                     <  1:878723/249‑1 (MQ=255)
                                                                                                                                               ttCTCCATTGAATTCAGTTAGGAGACTGTCGGCGTTGTGGATTCCGACCTGGTTGTCTGGTTTGCCGTCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCGGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGag                                                                                                    <  2:16174/249‑1 (MQ=255)
                                                                                                                                                tctcCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGTCAGCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGagt                                                                                                   <  2:909803/249‑1 (MQ=255)
                                                                                                                                                 nnccATCAGTTTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGGCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGagta                                                                                                  <  1:1255776/247‑1 (MQ=255)
                                                                                                                                                 ctcAATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGagtn                                                                                                  >  2:1269106/1‑248 (MQ=255)
                                                                                                                                                   ccATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGagtagt                                                                                                >  2:1203878/1‑249 (MQ=255)
                                                                                                                                                   ccATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGagtagt                                                                                                <  1:400263/249‑1 (MQ=255)
                                                                                                                                                      tCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGt                                                                                             <  2:1042014/249‑1 (MQ=255)
                                                                                                                                                          aTTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCgg                                                                                         >  1:464517/1‑249 (MQ=255)
                                                                                                                                                          aTTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCgg                                                                                         <  2:796499/249‑1 (MQ=255)
                                                                                                                                                             cAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCggtgg                                                                                      >  1:1042310/1‑249 (MQ=255)
                                                                                                                                                             cAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCggtgg                                                                                      >  2:405635/1‑249 (MQ=255)
                                                                                                                                                               gTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGgtggtg                                                                                    >  2:248467/1‑249 (MQ=255)
                                                                                                                                                                tGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGc                                                                                   >  2:1136569/1‑249 (MQ=255)
                                                                                                                                                                  aaGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCgg                                                                                 <  2:245656/249‑1 (MQ=255)
                                                                                                                                                                   agagACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCggg                                                                                <  2:136475/249‑1 (MQ=255)
                                                                                                                                                                      gaCTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAg                                                                             <  2:739883/249‑1 (MQ=255)
                                                                                                                                                                      gaCTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAg                                                                             <  1:582834/249‑1 (MQ=255)
                                                                                                                                                                       aCTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGGAGTGGTCCGGTGGTGcgggcggg                                                                            >  2:720898/1‑249 (MQ=255)
                                                                                                                                                                         tttCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGGCTTGGTGCCACTTCTGGAGGAGCATGCGAGGGAGTAGTGGTCCGGTGGTGGGGGCCgggg                                                                          >  1:660143/1‑249 (MQ=255)
                                                                                                                                                                             ggCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAg                                                                      >  2:159210/1‑249 (MQ=255)
                                                                                                                                                                             ggCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAg                                                                      <  2:1093636/249‑1 (MQ=255)
                                                                                                                                                                              gCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGc                                                                     <  1:147759/249‑1 (MQ=255)
                                                                                                                                                                               cGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGTCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGGGGGCAGGGGGTAGCg                                                                    <  1:1101773/249‑1 (MQ=255)
                                                                                                                                                                                    tgGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGa                                                               >  1:1267839/1‑249 (MQ=255)
                                                                                                                                                                                             aCCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGc                                                      <  2:625278/249‑1 (MQ=255)
                                                                                                                                                                                              ccTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGcc                                                     >  2:811491/1‑249 (MQ=255)
                                                                                                                                                                                                tGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCtg                                                   >  1:545145/1‑249 (MQ=255)
                                                                                                                                                                                                  gTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCtgtg                                                 <  1:1265746/249‑1 (MQ=255)
                                                                                                                                                                                                     gTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGc                                              >  2:453171/1‑249 (MQ=255)
                                                                                                                                                                                                     gTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGc                                              <  1:10405/249‑1 (MQ=255)
                                                                                                                                                                                                       cGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCAt                                            >  2:150645/1‑249 (MQ=255)
                                                                                                                                                                                                         gCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATaa                                          <  1:1343912/249‑1 (MQ=255)
                                                                                                                                                                                                            ttGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGCGTAGTGGTCCGGTGGTGCCGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAAttt                                       >  2:902542/1‑249 (MQ=255)
                                                                                                                                                                                                             tGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAAtttt                                      >  2:1328965/1‑249 (MQ=255)
                                                                                                                                                                                                                 aTCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTgaga                                  >  1:818967/1‑249 (MQ=255)
                                                                                                                                                                                                                     gTATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACaaa                              >  2:481949/1‑249 (MQ=255)
                                                                                                                                                                                                                      taggACTCCTGAGGAAATGGCAGAGCAGGATTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGAGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACaaaa                             <  2:1122271/246‑1 (MQ=255)
                                                                                                                                                                                                                      tATGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACaaaa                             <  1:482702/249‑1 (MQ=255)
                                                                                                                                                                                                                       aTGACTCCTGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAaat                            <  1:1191323/249‑1 (MQ=255)
                                                                                                                                                                                                                               tGAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCGCTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGaaa                    >  2:955489/1‑249 (MQ=255)
                                                                                                                                                                                                                               ngAGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGaaa                    <  1:946435/248‑1 (MQ=255)
                                                                                                                                                                                                                                 aGGAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACg                  <  2:107815/249‑1 (MQ=255)
                                                                                                                                                                                                                                  ggAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGt                 >  2:579355/1‑249 (MQ=255)
                                                                                                                                                                                                                                   gAAATGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGtc                >  1:1345373/1‑249 (MQ=255)
                                                                                                                                                                                                                                    aaaTGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGtct               >  1:643583/1‑249 (MQ=255)
                                                                                                                                                                                                                                     aaTGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGtctc              <  2:542856/249‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGCAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGtctcnn            >  1:832045/1‑247 (MQ=255)
                                                                                                                                                                                                                                          cAGAGCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATg         >  1:716979/1‑249 (MQ=255)
                                                                                                                                                                                                                                            gagCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGtt       <  1:592716/249‑1 (MQ=255)
                                                                                                                                                                                                                                             agCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTg      <  1:16656/249‑1 (MQ=255)
                                                                                                                                                                                                                                             agCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTg      >  1:1213885/1‑249 (MQ=255)
                                                                                                                                                                                                                                             agCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTg      >  1:983544/1‑249 (MQ=255)
                                                                                                                                                                                                                                              gCAGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTgg     <  2:588817/249‑1 (MQ=255)
                                                                                                                                                                                                                                                aGGTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTGGGa   >  1:422379/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 ggTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTGGGan  >  2:1358367/1‑248 (MQ=255)
                                                                                                                                                                                                                                                 ggTTTCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTGGGaa  <  1:174047/249‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
CTGAGAAGACCGCGGCTGCTGTATCTGCAACGGCAACCAGCTGGGGTGCATACATCAAGGGCTCCAACCGTGTAGATACTTTGCTGGACCTGGGCTTCCAGGAAAACCCTGAGCTGGCTAAACAGCAACCTGGCGATACGGGTTTCTCCATCAAATTCAGTGAAGAGACTTTCGGCGTTGTGGATTCCGACCTGGTTGTCGGCTTTGCCATCGGTATGACTCCTGAGGAAATGGCAGAGCAGGTTCCATGGCAGATGTTGACCGCCACTCGTGACGGCCGTTCCTTTGTGATGCCCCGTGAGATTTCCAATGCGTTTTCTTTGGGTTCCCCGCAGTCCACTCGGTTCGCGTTAGACGCCTTGGTGCCACTTCTGGAGGAGCATGCAGGGGAGTAGTGGTCCGGTGGTGCGGGCAGGGGGTAGCGGTCGAATTATTTGCCTGTGGGCATAATTTTGAGACAAAATAATAGAAACGTCTCAGATGTTGGGAA  >  CP025533/1324747‑1325236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑