Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 947,066 (T)6→7 coding (306/1755 nt) CYL77_04560 → BCCT family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP025533947,0601.T99.1% 451.4 / ‑2.9 110coding (300/1755 nt)CYL77_04560BCCT family transporter
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (52/57);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 115 total aligned reads displayed.

CTTGAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGC  >  CP025533/946815‑947301
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
cttgAGAATCCAACTAACCTGGAAGGGAAACTGGCTGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑t                                                                                                                                                                                                                                                <  1:212640/249‑1 (MQ=255)
 ttgAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑tt                                                                                                                                                                                                                                               >  2:101409/1‑249 (MQ=255)
   gagaATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑tttt                                                                                                                                                                                                                                             <  2:505144/249‑1 (MQ=255)
       aTCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑tt‑tttttatc                                                                                                                                                                                                                                         <  1:42194/249‑5 (MQ=255)
                   tGGAAGGGAAACTGGCCGACGCAGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCTGCAGTAGTTGACAATCTCGGCTGGGCCTTCAGTTTGTTGGGCTCAGTCTTTGTATTT‑TTTTATCGTTGTTATcgcc                                                                                                                                                                                                                              >  2:1171578/1‑249 (MQ=255)
                    ggAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATcgccg                                                                                                                                                                                                                             <  1:45069/249‑1 (MQ=255)
                    ggAAGGGAAACTGGCCGACGACGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATcgccg                                                                                                                                                                                                                             <  1:185671/249‑1 (MQ=255)
                              cTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTAGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAAtt                                                                                                                                                                                                                   >  2:1209652/1‑249 (MQ=255)
                               nggCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTc                                                                                                                                                                                                                  <  2:507046/248‑1 (MQ=255)
                               nggCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTc                                                                                                                                                                                                                  <  2:1280121/248‑1 (MQ=255)
                                ggCCGACGCCGAAGAGGAAATTATCCCCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCg                                                                                                                                                                                                                 <  2:12339/249‑1 (MQ=255)
                                  ccGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGc                                                                                                                                                                                                               <  1:595749/249‑1 (MQ=255)
                                  ccGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGc                                                                                                                                                                                                               <  1:314030/249‑1 (MQ=255)
                                     aCGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACg                                                                                                                                                                                                            >  2:615048/1‑249 (MQ=255)
                                      cGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGa                                                                                                                                                                                                           >  2:1172254/1‑249 (MQ=255)
                                        ccGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGAtt                                                                                                                                                                                                         <  1:307989/249‑1 (MQ=255)
                                         cGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTc                                                                                                                                                                                                        <  1:786803/249‑1 (MQ=255)
                                                gacaTTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAgg                                                                                                                                                                                                 <  2:644962/246‑1 (MQ=255)
                                                  aaTTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGcc                                                                                                                                                                                               >  2:855654/1‑249 (MQ=255)
                                                    ttATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGGTTCGCTTAGGCCGc                                                                                                                                                                                             >  1:1142725/1‑249 (MQ=255)
                                                    ttATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGc                                                                                                                                                                                             >  1:351213/1‑249 (MQ=255)
                                                          ncGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgat                                                                                                                                                                                       <  1:1141251/248‑1 (MQ=255)
                                                           cGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATtgatg                                                                                                                                                                                      <  1:185952/249‑1 (MQ=255)
                                                              aGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAg                                                                                                                                                                                   >  2:808651/1‑249 (MQ=255)
                                                                  gAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCAcc                                                                                                                                                                               >  2:539470/1‑249 (MQ=255)
                                                                    aGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCag                                                                                                                                                                             <  2:680629/249‑1 (MQ=255)
                                                                      acacCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCagag                                                                                                                                                                           >  1:424154/1‑249 (MQ=255)
                                                                      acacCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TTATTTTATCGTTGTTATCGCAGCTAATAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCagag                                                                                                                                                                           >  2:531678/1‑249 (MQ=255)
                                                                       cacCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGt                                                                                                                                                                          >  2:535971/1‑249 (MQ=255)
                                                                         cccAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGttt                                                                                                                                                                        <  1:1106370/249‑1 (MQ=255)
                                                                           cAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCg                                                                                                                                                                      >  2:956652/1‑249 (MQ=255)
                                                                               ccTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACg                                                                                                                                                                  <  1:963887/249‑1 (MQ=255)
                                                                                   aCTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGgtgt                                                                                                                                                              >  2:1027617/1‑249 (MQ=255)
                                                                                     ntAACTGGTCAGTCATCGTTCCAGCCCGAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCa                                                                                                                                                            <  2:213602/248‑1 (MQ=255)
                                                                                          nggTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGAt                                                                                                                                                       <  1:656571/248‑1 (MQ=255)
                                                                                           ggTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGAtt                                                                                                                                                      <  2:1073150/249‑1 (MQ=255)
                                                                                               agtcaTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCa                                                                                                                                                  <  1:398558/249‑1 (MQ=255)
                                                                                                  caTCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCatga                                                                                                                                               <  1:263919/249‑1 (MQ=255)
                                                                                                     cGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGt                                                                                                                                            >  1:923351/1‑249 (MQ=255)
                                                                                                              ccTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAg                                                                                                                                   >  2:752566/1‑249 (MQ=255)
                                                                                                              ccTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAg                                                                                                                                   >  1:157910/1‑249 (MQ=255)
                                                                                                               cTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAgg                                                                                                                                  <  2:371262/249‑1 (MQ=255)
                                                                                                                 aGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTa                                                                                                                                <  2:621477/249‑1 (MQ=255)
                                                                                                                     aTTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATggg                                                                                                                            >  2:640543/1‑249 (MQ=255)
                                                                                                                       tGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTa                                                                                                                          >  2:746461/1‑249 (MQ=255)
                                                                                                                         tCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTAtt                                                                                                                        <  2:938527/249‑1 (MQ=255)
                                                                                                                         tCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTAtt                                                                                                                        <  1:701483/249‑1 (MQ=255)
                                                                                                                           nnaGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTgg                                                                                                                      <  1:874491/247‑1 (MQ=255)
                                                                                                                           cTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTgg                                                                                                                      >  1:926797/1‑249 (MQ=255)
                                                                                                                                gACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGa                                                                                                                 >  2:444417/1‑249 (MQ=255)
                                                                                                                                      ggtgTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCt                                                                                                           <  1:773883/249‑1 (MQ=255)
                                                                                                                                        tgtgGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTAc                                                                                                         <  1:615048/249‑1 (MQ=255)
                                                                                                                                         gtgGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACg                                                                                                        <  2:437505/249‑1 (MQ=255)
                                                                                                                                           ggggTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGa                                                                                                      <  1:115306/249‑1 (MQ=255)
                                                                                                                                            gggTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGaa                                                                                                     >  2:1094757/1‑249 (MQ=255)
                                                                                                                                              gTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAAcc                                                                                                   <  2:745958/249‑1 (MQ=255)
                                                                                                                                              gTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAAcc                                                                                                   <  2:172128/249‑1 (MQ=255)
                                                                                                                                                 tCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACcaca                                                                                                <  1:101409/249‑1 (MQ=255)
                                                                                                                                                     aTTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAAc                                                                                            >  1:781294/1‑249 (MQ=255)
                                                                                                                                                     aTTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAAc                                                                                            >  1:552443/1‑249 (MQ=255)
                                                                                                                                                      ttCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAAcc                                                                                           >  2:737546/1‑249 (MQ=255)
                                                                                                                                                        cAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCtt                                                                                         <  2:980454/249‑1 (MQ=255)
                                                                                                                                                        cAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCtt                                                                                         >  1:1310803/1‑249 (MQ=255)
                                                                                                                                                         aaaGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCttt                                                                                        <  1:820244/249‑1 (MQ=255)
                                                                                                                                                            gATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAAc                                                                                     >  1:28305/1‑249 (MQ=255)
                                                                                                                                                                gCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTc                                                                                 >  2:395343/1‑249 (MQ=255)
                                                                                                                                                                 cTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCt                                                                                >  1:923843/1‑249 (MQ=255)
                                                                                                                                                                     aCCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCg                                                                            <  1:1318898/249‑1 (MQ=255)
                                                                                                                                                                       cAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCa                                                                          >  1:1096114/1‑249 (MQ=255)
                                                                                                                                                                        aaCTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCaa                                                                         >  1:327498/1‑249 (MQ=255)
                                                                                                                                                                          cTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAAt                                                                        >  2:942994/1‑248 (MQ=255)
                                                                                                                                                                          cTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAAt                                                                        <  1:942994/248‑1 (MQ=255)
                                                                                                                                                                           nttGCTAGTTCTGCGTTGTCCGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATgg                                                                      <  2:798164/248‑1 (MQ=255)
                                                                                                                                                                             tGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGgtg                                                                    >  2:710518/1‑249 (MQ=255)
                                                                                                                                                                               tcagtgtggctttGTCGTCAGTAGTTGCCAAGCTCGCTTGGTCCTGCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTACCTCCGCTAGTAAATTCGCCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTa                                                                  <  2:480923/238‑1 (MQ=255)
                                                                                                                                                                               cTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTa                                                                  <  1:997274/249‑1 (MQ=255)
                                                                                                                                                                                  gTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCt                                                               >  2:1019876/1‑249 (MQ=255)
                                                                                                                                                                                   ttCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTg                                                              <  1:872515/249‑1 (MQ=255)
                                                                                                                                                                                    tCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTgg                                                             <  1:463572/249‑1 (MQ=255)
                                                                                                                                                                                    tCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTgg                                                             >  1:427253/1‑249 (MQ=255)
                                                                                                                                                                                       gCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCCTGGATTTCCATGATGTTTGCTGCAGGTCTGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAAACTTCTACCGCAATGGTGTACCTGGACa                                                          >  2:978831/1‑249 (MQ=255)
                                                                                                                                                                                         gTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCTCAATGGTGTACCTGTACatg                                                        >  1:990951/1‑249 (MQ=255)
                                                                                                                                                                                          ttGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACatga                                                       <  1:657892/249‑1 (MQ=255)
                                                                                                                                                                                           tGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACatgat                                                      <  1:590796/249‑1 (MQ=255)
                                                                                                                                                                                           tGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTAAGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACatgat                                                      >  2:249519/1‑249 (MQ=255)
                                                                                                                                                                                                   gtagtTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACaa                                              >  2:232167/1‑249 (MQ=255)
                                                                                                                                                                                                          aCAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGc                                       <  1:798753/249‑1 (MQ=255)
                                                                                                                                                                                                             aTCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGtt                                    >  2:210149/1‑249 (MQ=255)
                                                                                                                                                                                                               ctcGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGc                                  >  2:33325/1‑249 (MQ=255)
                                                                                                                                                                                                                 cGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTa                                >  2:961943/1‑249 (MQ=255)
                                                                                                                                                                                                                  ggCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTAt                               >  2:1153618/1‑249 (MQ=255)
                                                                                                                                                                                                                  ggCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTACTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTAt                               >  2:439366/1‑249 (MQ=255)
                                                                                                                                                                                                                     tGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATg                              <  2:356675/247‑1 (MQ=255)
                                                                                                                                                                                                                     tGGGCCTTCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATg                              >  1:356675/1‑247 (MQ=255)
                                                                                                                                                                                                                            tCATTTTGTTTGGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACa                     <  1:588763/249‑1 (MQ=255)
                                                                                                                                                                                                                                  tgtttgGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTc               >  2:859362/1‑249 (MQ=255)
                                                                                                                                                                                                                                      tgGCACAGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACt           <  1:961943/249‑1 (MQ=255)
                                                                                                                                                                                                                                            aGTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCt     <  1:86634/249‑1 (MQ=255)
                                                                                                                                                                                                                                             gTCTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCtt    <  1:1114503/249‑1 (MQ=255)
                                                                                                                                                                                                                                               cTTTGTATTT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGc  >  1:822346/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
CTTGAGAATCCAACTAACCTGGAAGGGAAACTGGCCGACGCCGAAGAGGAAATTATCCTCGAAGGCGAAGACACCCAGGCCTCACTTAACTGGTCAGTCATCGTTCCAGCCCTAGTCATTGTCCTAGCGACAGTGGTGTGGGGTATCGGATTCAAAGATAGCTTTACCAACTTTGCTAGTTCTGCGTTGTCAGCAGTAGTTGACAATCTCGGCTGGGCCTTCATTTTGTTTGGCACAGTCTTTGTA‑TT‑TTTTATCGTTGTTATCGCCGCTAGTAAATTCGGCACGATTCGCTTAGGCCGCATTGATGAAGCACCAGAGTTTCGCACGGTGTCATGGATTTCCATGATGTTTGCTGCAGGTATGGGTATTGGTTTGATGTTCTACGGAACCACAGAACCTTTAACCTTCTACCGCAATGGTGTACCTGGACATGATGAACACAATGTTGGCGTTGCTATGTCCACGACAATGTTCCACTGGACCTTGC  >  CP025533/946815‑947301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑