BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP025533	2,084,510	0	T	A	49.8%	‑1.7 / inf	253	intergenic (‑12/+189)	dapA/CYL77_09945	4‑hydroxy‑tetrahydrodipicolinate synthase/thymidylate synthase (FAD)
Reads supporting (aligned to +/- strand): ref base T (59/68); new base A (59/67); total (118/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

AACTTCCTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGCCAAAGTTTCAA  >  CP025533/2084262‑2084757

aaCTTCCTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTTAGCTCATAGAGTTCAAGGt                                                                                                                                                                                                                                                           >  2:1015891/1‑249 (MQ=255)

 aCTTCCTCACGAACGGCCTCGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCAACAGTGGTGCGCGCGAGAACCAAAGAATCCAAGCCCTTATAAACAAAATAAGCAGCGACTTCGCGGCCAGCAGCGATACCGATGTCGCCGGATTCCGTGAAGGGAGTAACCATTGCTAATCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTAATAGAGTTCAAGGtt                                                                                                                                                                                                                                                          <  2:740784/249‑1 (MQ=255)

     ccTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTACTCATAGAGTTCAAGGTTACct                                                                                                                                                                                                                                                      >  2:324521/1‑249 (MQ=255)

           gAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTcc                                                                                                                                                                                                                                                >  2:1174679/1‑249 (MQ=255)

           gAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTcc                                                                                                                                                                                                                                                >  1:319783/1‑249 (MQ=255)

             aCGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCt                                                                                                                                                                                                                                              <  2:274979/249‑1 (MQ=255)

              cGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTc                                                                                                                                                                                                                                             <  1:645558/249‑1 (MQ=255)

                  cTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑ttt                                                                                                                                                                                                                                        >  1:289560/1‑249 (MQ=255)

                           ttCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTa                                                                                                                                                                                                                               <  2:75377/249‑1 (MQ=255)

                           ttCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTaa                                                                                                                                                                                                                              >  1:854780/1‑249 (MQ=255)

                                 tttttCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAAcc                                                                                                                                                                                                                        <  2:1318070/249‑1 (MQ=255)

                                       aGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCa                                                                                                                                                                                                                   <  1:404718/249‑1 (MQ=255)

                                          ggcggTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCaaaa                                                                                                                                                                                                                <  2:212670/249‑1 (MQ=255)

                                          ggcggTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCaaaa                                                                                                                                                                                                                >  1:1141154/1‑249 (MQ=255)

                                          ggcggTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCaaaaa                                                                                                                                                                                                               <  1:958472/249‑1 (MQ=255)

                                            cggTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCaaaaaa                                                                                                                                                                                                              <  1:411888/249‑1 (MQ=255)

                                             ggTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAAc                                                                                                                                                                                                             >  2:492379/1‑249 (MQ=255)

                                                 gTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTaac                                                                                                                                                                                                        <  1:528755/249‑1 (MQ=255)

                                                   cGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTaaca                                                                                                                                                                                                       >  1:1095886/1‑249 (MQ=255)

                                                      tGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACtt                                                                                                                                                                                                   >  2:146505/1‑249 (MQ=255)

                                                             tCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATa                                                                                                                                                                                            <  1:322095/249‑1 (MQ=255)

                                                                acAGTCGTTCCCGCCAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAAc                                                                                                                                                                                          <  2:1033152/248‑1 (MQ=255)

                                                                 cAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACaa                                                                                                                                                                                        <  2:384295/249‑1 (MQ=255)

                                                                  aGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACaa                                                                                                                                                                                        <  2:111843/249‑1 (MQ=255)

                                                                  aGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAAt                                                                                                                                                                                       >  2:1006993/1‑249 (MQ=255)

                                                                    tggtgCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATc                                                                                                                                                                                      <  2:365394/249‑1 (MQ=255)

                                                                    tggtgCCCGCCAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCg                                                                                                                                                                                     <  1:516616/249‑1 (MQ=255)

                                                                           cgcgAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAg                                                                                                                                                                               >  1:735024/1‑249 (MQ=255)

                                                                            gcgAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGc                                                                                                                                                                              >  2:1261076/1‑249 (MQ=255)

                                                                               agaACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCTACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCcac                                                                                                                                                                           <  2:817501/249‑1 (MQ=255)

                                                                                  aCCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACAtt                                                                                                                                                                        >  2:845094/1‑249 (MQ=255)

                                                                                   ccAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTc                                                                                                                                                                       <  2:469447/249‑1 (MQ=255)

                                                                                    cAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTcc                                                                                                                                                                      <  2:237393/249‑1 (MQ=255)

                                                                                      aaGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGa                                                                                                                                                                    >  1:381930/1‑249 (MQ=255)

                                                                                         aaTCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATaa                                                                                                                                                                 <  2:925801/249‑1 (MQ=255)

                                                                                                 cccTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATACAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTg                                                                                                                                                        <  1:491240/249‑1 (MQ=255)

                                                                                                  ccTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTg                                                                                                                                                        <  1:674088/249‑1 (MQ=255)

                                                                                                         aaCCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAg                                                                                                                                                 <  1:30065/249‑1 (MQ=255)

                                                                                                           ccAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCan                                                                                                                                              >  1:1067672/1‑248 (MQ=255)

                                                                                                            cAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCann                                                                                                                                             >  1:21113/1‑247 (MQ=255)

                                                                                                              aaTCAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCATTTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAaga                                                                                                                                            <  2:90335/249‑1 (MQ=255)

                                                                                                                  aGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCGCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACCTTTGCATAACAATCGCCCAAGCCACCTTCAGATAAGAACACTTGACACCAGCAAGAGGaa                                                                                                                                        <  2:615461‑M2/249‑3 (MQ=255)

                                                                                                                  aGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGttt                                                                                                                                       >  1:480299/1‑249 (MQ=255)

                                                                                                                     cgcgACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattc                                                                                                                                     <  1:348604‑M2/249‑6 (MQ=255)

                                                                                                                     cgcgACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattc                                                                                                                                     >  1:1246999‑M2/1‑244 (MQ=255)

                                                                                                                     cgcgACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTa                                                                                                                                    <  1:130689/249‑1 (MQ=255)

                                                                                                                          cTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCt                                                                                                                               >  2:949635/1‑249 (MQ=255)

                                                                                                                            tCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGt                                                                                                                             >  1:587551/1‑249 (MQ=255)

                                                                                                                               cgGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTATACAACCCCGCAAAAAACCTAACAACTTTTGCATAACCATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggca                                                                                                                           >  2:1210619‑M2/1‑234 (MQ=255)

                                                                                                                               cgGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggca                                                                                                                           <  1:1128129‑M2/249‑16 (MQ=255)

                                                                                                                                    agcagcGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTAcn                                                                                                                     >  1:1260687/1‑248 (MQ=255)

                                                                                                                                     gcagcGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAg                                                                                                                    <  1:779949/249‑1 (MQ=255)

                                                                                                                                     gcagcGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTACCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgaca                                                                                                                     >  2:268750‑M2/1‑228 (MQ=255)

                                                                                                                                      cagcGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGc                                                                                                                   >  1:265474/1‑249 (MQ=255)

                                                                                                                                          gATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttg                                                                                                                >  1:717873‑M2/1‑223 (MQ=255)

                                                                                                                                                   gTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTc                                                                                                      <  2:640371/249‑1 (MQ=255)

                                                                                                                                                       ccGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTCACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTaa                                                                                                  <  2:554674/249‑1 (MQ=255)

                                                                                                                                                        cGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAAc                                                                                                 >  1:832260/1‑249 (MQ=255)

                                                                                                                                                          gATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACta                                                                                               >  1:692411/1‑249 (MQ=255)

                                                                                                                                                           aTTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgat                                                                                               >  1:807828‑M2/1‑206 (MQ=255)

                                                                                                                                                               cGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctg                                                                                           <  1:766879‑M2/249‑48 (MQ=255)

                                                                                                                                                                 tGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAt                                                                                        >  1:1021651/1‑249 (MQ=255)

                                                                                                                                                                 tGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAt                                                                                        <  1:1150016/249‑1 (MQ=255)

                                                                                                                                                                  gAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgaga                                                                                        <  1:1189949‑M2/249‑51 (MQ=255)

                                                                                                                                                                  gAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAAtt                                                                                       <  1:369277/249‑1 (MQ=255)

                                                                                                                                                                    aTGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaag                                                                                      >  1:630154‑M2/1‑197 (MQ=255)

                                                                                                                                                                      ggAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcactgacagtttaagacggacaaaggcgattcttagcagcc                                                                                    <  2:806098‑M2/249‑55 (MQ=255)

                                                                                                                                                                      ggAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCAGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTa                                                                                   <  2:1052709/249‑1 (MQ=255)

                                                                                                                                                                       gAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagcca                                                                                   >  1:88663‑M2/1‑194 (MQ=255)

                                                                                                                                                                         gTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccatt                                                                                 <  1:260518‑M2/249‑58 (MQ=255)

                                                                                                                                                                         gTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccatt                                                                                 >  2:171609‑M2/1‑192 (MQ=255)

                                                                                                                                                                         gTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCt                                                                                <  2:525850/249‑1 (MQ=255)

                                                                                                                                                                            aCCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttc                                                                              >  1:1082088‑M2/1‑189 (MQ=255)

                                                                                                                                                                                ttGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttg                                                                          >  1:1137670‑M2/1‑185 (MQ=255)

                                                                                                                                                                                   cTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggc                                                                       <  1:1171134‑M2/249‑68 (MQ=255)

                                                                                                                                                                                          aaCGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTg                                                               >  2:556513/1‑249 (MQ=255)

                                                                                                                                                                                             ggTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcag                                                             >  1:508352‑M2/1‑172 (MQ=255)

                                                                                                                                                                                             ggTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAAACTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGCTAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtatgagacggccacagtcgattctgcgaatccattttctttggccgccgtggcag                                                             <  1:592128‑M2/249‑78 (MQ=255)

                                                                                                                                                                                               tGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtt                                                           >  2:1290535‑M2/1‑170 (MQ=255)

                                                                                                                                                                                               tGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTa                                                          <  2:1067672/249‑1 (MQ=255)

                                                                                                                                                                                                      gTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattg                                                    >  1:875873‑M2/1‑163 (MQ=255)

                                                                                                                                                                                                        gCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCAt                                                 <  2:821220/249‑1 (MQ=255)

                                                                                                                                                                                                          nctACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGa                                               <  2:437279/248‑1 (MQ=255)

                                                                                                                                                                                                            tACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtccc                                              <  1:544343‑M2/249‑93 (MQ=255)

                                                                                                                                                                                                               tCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccacc                                           <  1:1223067‑M2/249‑96 (MQ=255)

                                                                                                                                                                                                               nccGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATAc                                          <  2:684896/248‑1 (MQ=255)

                                                                                                                                                                                                                   gTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGc                                      >  2:335911/1‑249 (MQ=255)

                                                                                                                                                                                                                      ttAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCttg                                   >  2:1190730/1‑249 (MQ=255)

                                                                                                                                                                                                                          cTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGc                               >  2:837095/1‑249 (MQ=255)

                                                                                                                                                                                                                               aaaCCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattc                           >  1:167523‑M2/1‑138 (MQ=255)

                                                                                                                                                                                                                                aaCCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcg                          >  2:713851‑M2/1‑137 (MQ=255)

                                                                                                                                                                                                                                aaCCTGTGCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcg                          >  1:561122‑M2/1‑137 (MQ=255)

                                                                                                                                                                                                                                   cTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGa                      >  1:228441/1‑249 (MQ=255)

                                                                                                                                                                                                                                     gtgCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATc                    <  1:140156/249‑1 (MQ=255)

                                                                                                                                                                                                                                      tgCTCATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcgaaccan                    <  1:176962‑M2/249‑119 (MQ=255)

                                                                                                                                                                                                                                      tgCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATcc                   <  2:42910/249‑1 (MQ=255)

                                                                                                                                                                                                                                          cATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcgaaccaaggag                >  2:916133‑M2/1‑127 (MQ=255)

                                                                                                                                                                                                                                          cATAGAGTTCAATTATACCTTCTTCCCTCA‑TTTTGGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGaattcgcgtatggcaatgacagtttgagacggccacaggcgattctgagaagccattttctttgggcgccgtggcagtttttattgggtcccaccgccgaactgcatattcgaaccaaggag                <  2:976163‑M2/249‑123 (MQ=255)

                                                                                                                                                                                                                                                 ttCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGCCAAAg        <  1:1006993/249‑1 (MQ=255)

                                                                                                                                                                                                                                                       gTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGCATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGCCAAAGTTTCaa  >  2:1294547/1‑249 (MQ=255)

AACTTCCTCACGAACGGCCTTGAGCAGTTCTAGTTTTTCAGCGGCGGTTGTCGTTGGGGATTCACCAGTGGTGCCCGCGAGAACCAAAGAATCCAAGCCCTTATCAACCAAATAAGCCGCGACTTCGCGGCCAGCAGCGATATCGATGTCTCCGGATTCCGTGAATGGAGTAACCATTGCTACTCCAACGGTGCCGAAGTGCTCTACTCCGGTCTTAGCTGTTAAACCTGTGCTCATAGAGTTCAAGGTTACCTTCTTCCCTCA‑TTT‑GGGGGTTAAACAACCCCGCAAAAAACCTAACAACTTTTGCATAACAATCGCCCAAGCCACATTCCGATAAGAACAATTGACACGAGCAAGAGGTTTTTACGGCTGTCTTTTACAGCCAAAAATGTTGTCTTAACTATATGAATTTTTAGCTCGTGGGTGTGAGCTTTGCGTTAAAAGTCCATGACATACGGGCTTGTTGCCATTTGCGATCCGTCTGCCAAAGTTTCAA  >  CP025533/2084262‑2084757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`