Predicted mutation
evidence seq id position mutation annotation gene description
RA CP025533 576,540 A→C K65T (AAG→ACG)  CYL77_02885 → 30S ribosomal protein S4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP025533576,5400AC99.3% 528.4 / NA 135K65T (AAG→ACG) CYL77_0288530S ribosomal protein S4
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (76/58);  total (76/59)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 135 total aligned reads displayed.

CCGTCCACCAAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAAAGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCAGAAG  >  CP025533/576296‑576784
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
ccGTCCACCAAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAg                                                                                                                                                                                                                                                  >  2:652583/1‑249 (MQ=255)
  gTCCACCAAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGtt                                                                                                                                                                                                                                                <  1:606822/249‑1 (MQ=255)
   tCCACCAAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTc                                                                                                                                                                                                                                               >  1:199286/1‑249 (MQ=255)
    ccaccaAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTcc                                                                                                                                                                                                                                              <  2:555885/249‑1 (MQ=255)
     caccaAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCcg                                                                                                                                                                                                                                             <  1:785267/249‑1 (MQ=255)
       ccaAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCACCCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGACTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCAGGGAAACGCCGTTCcgtc                                                                                                                                                                                                                                           >  2:890256/1‑249 (MQ=255)
         aaaGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCcgtcgt                                                                                                                                                                                                                                         >  2:576073/1‑249 (MQ=255)
          aaGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGtt                                                                                                                                                                                                                                        >  1:1171087/1‑249 (MQ=255)
          aaGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGtt                                                                                                                                                                                                                                        >  2:646155/1‑249 (MQ=255)
          aaGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGtt                                                                                                                                                                                                                                        <  1:156105/249‑1 (MQ=255)
               tcgtcgCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTtactac                                                                                                                                                                                                                                   <  2:1138891/249‑1 (MQ=255)
                cgtcgCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACg                                                                                                                                                                                                                                  >  2:763511/1‑249 (MQ=255)
                 gtcgCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGc                                                                                                                                                                                                                                 >  1:1221502/1‑249 (MQ=255)
                  tcgCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGcc                                                                                                                                                                                                                                <  2:954084/249‑1 (MQ=255)
                  tcgCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGcc                                                                                                                                                                                                                                <  2:704962/249‑1 (MQ=255)
                             aGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATGCTGTCGTCTGCGCGCCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCACCAAGGAGTCCGAGTACGTGCTGCAGCTCCAGGAGAAGCAGAAGGCCCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCg                                                                                                                                                                                                                     >  2:1061896/1‑249 (MQ=255)
                             aGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCg                                                                                                                                                                                                                     <  1:474228/249‑1 (MQ=255)
                               ggaaggaaAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTc                                                                                                                                                                                                                   >  1:503442/1‑249 (MQ=255)
                                gaaggaaAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTcg                                                                                                                                                                                                                  >  2:698360/1‑249 (MQ=255)
                                  aggaaAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTcgcg                                                                                                                                                                                                                >  2:51466/1‑249 (MQ=255)
                                     aaaGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAg                                                                                                                                                                                                             >  1:662315/1‑249 (MQ=255)
                                     aaaGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAg                                                                                                                                                                                                             >  1:1240076/1‑249 (MQ=255)
                                       aGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGc                                                                                                                                                                                                           >  2:672523/1‑249 (MQ=255)
                                       aGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGc                                                                                                                                                                                                           >  1:953386/1‑249 (MQ=255)
                                         gTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCan                                                                                                                                                                                                         >  1:1268262/1‑248 (MQ=255)
                                            aTACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGAc                                                                                                                                                                                                      >  1:414492/1‑249 (MQ=255)
                                              aCAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCGGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCg                                                                                                                                                                                                    >  1:670483/1‑249 (MQ=255)
                                                aaaaTGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGt                                                                                                                                                                                                  >  2:904748/1‑249 (MQ=255)
                                                 aaaTGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTg                                                                                                                                                                                                 >  1:199512/1‑249 (MQ=255)
                                                  aaTGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGATCGGTgn                                                                                                                                                                                                >  1:1149127/1‑248 (MQ=255)
                                                   aTGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTGTGCGCGTCGACCTTGTAGGTGGAGACATGGCGGTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGATTCATCTACGGCGTCATGCAAACGCAGTTACGTCGTTACTACGCAGCGGCTAACCGTCGCGCAGGCAAGACCGGTgnn                                                                                                                                                                                               >  1:569626/1‑247 (MQ=255)
                                                      gCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAAc                                                                                                                                                                                            <  2:1171087/249‑1 (MQ=255)
                                                        tCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCt                                                                                                                                                                                          >  2:285634/1‑249 (MQ=255)
                                                        ncGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCt                                                                                                                                                                                          <  2:840997/248‑1 (MQ=255)
                                                          gTTATAACGGCCCAGCAACCAGTAGACCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCGCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTgg                                                                                                                                                                                        <  2:101102/249‑1 (MQ=255)
                                                                 cGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCt                                                                                                                                                                                 <  2:553931/249‑1 (MQ=255)
                                                                  ggCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTg                                                                                                                                                                                >  2:445991/1‑249 (MQ=255)
                                                                     ccAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTc                                                                                                                                                                             >  2:1287574/1‑249 (MQ=255)
                                                                       aGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGa                                                                                                                                                                           <  1:797633/249‑1 (MQ=255)
                                                                         cAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAACCGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGt                                                                                                                                                                         >  2:648831/1‑249 (MQ=255)
                                                                           aCCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTcc                                                                                                                                                                       >  2:615219/1‑249 (MQ=255)
                                                                                    tCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGAc                                                                                                                                                              <  1:94187/249‑1 (MQ=255)
                                                                                     cccGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACa                                                                                                                                                             >  1:941611/1‑249 (MQ=255)
                                                                                      ccGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACaa                                                                                                                                                            >  2:1187821/1‑249 (MQ=255)
                                                                                       cgtcgtCTGCGCGTCGCCCTTGTTGGTGGAGACAGGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAAc                                                                                                                                                           <  1:666597/249‑1 (MQ=255)
                                                                                         tcgtcTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAAcgt                                                                                                                                                         >  2:1037309/1‑249 (MQ=255)
                                                                                                       aCCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAg                                                                                                                                           >  1:974250/1‑249 (MQ=255)
                                                                                                         cTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGt                                                                                                                                         >  2:112889/1‑249 (MQ=255)
                                                                                                            gttgGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTg                                                                                                                                      <  1:1260217/249‑1 (MQ=255)
                                                                                                                gtggAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCaa                                                                                                                                  >  1:214327/1‑249 (MQ=255)
                                                                                                                     gaCATGGCGTTTGAGCGCCGACCTTACCCTTCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACAcc                                                                                                                             <  1:487726/249‑1 (MQ=255)
                                                                                                                             gTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGccn                                                                                                                     >  2:492540/1‑248 (MQ=255)
                                                                                                                                 gAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCt                                                                                                                 >  2:698614/1‑249 (MQ=255)
                                                                                                                                           cTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTg                                                                                                       <  1:889627/249‑1 (MQ=255)
                                                                                                                                           cTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCAACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTg                                                                                                       >  1:560726/1‑249 (MQ=255)
                                                                                                                                                  tCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCa                                                                                                <  2:1088840/249‑1 (MQ=255)
                                                                                                                                                          aggcaggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACt                                                                                        >  2:1221894/1‑249 (MQ=255)
                                                                                                                                                          aggcaggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACt                                                                                        <  1:376357/249‑1 (MQ=255)
                                                                                                                                                            gcaggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTc                                                                                      <  1:604237/249‑1 (MQ=255)
                                                                                                                                                             caggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCa                                                                                     >  2:279779/1‑249 (MQ=255)
                                                                                                                                                              aggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCAc                                                                                    >  1:322007/1‑249 (MQ=255)
                                                                                                                                                              aggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCAc                                                                                    <  1:342982/249‑1 (MQ=255)
                                                                                                                                                               ggcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCAcc                                                                                   >  1:1025949/1‑249 (MQ=255)
                                                                                                                                                                gcCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGCTTCCCACGGTCACTTCACCg                                                                                  <  1:576073/249‑1 (MQ=255)
                                                                                                                                                                    tGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGaa                                                                              <  2:970881/249‑1 (MQ=255)
                                                                                                                                                                         gCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACggca                                                                         <  2:566185/249‑1 (MQ=255)
                                                                                                                                                                           aTCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACggcaag                                                                       >  2:148518/1‑249 (MQ=255)
                                                                                                                                                                            tCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACggcaagg                                                                      >  2:1055514/1‑249 (MQ=255)
                                                                                                                                                                            tCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACggcaagg                                                                      >  2:598605/1‑249 (MQ=255)
                                                                                                                                                                            tCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACggcaagg                                                                      >  1:1178317/1‑249 (MQ=255)
                                                                                                                                                                                ggAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAAt                                                                  >  2:739915/1‑249 (MQ=255)
                                                                                                                                                                                     ccGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACg                                                             >  2:811400/1‑249 (MQ=255)
                                                                                                                                                                                        aGTACCTGCTGCAGCTCCAGGAGAATGAGAAGGCTCGTTTCATCGACGGCGTCATGGAAAAGCAGTTCGGTCGTTACTATGCCGAGGCGAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCGCGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTc                                                          <  2:114610/249‑1 (MQ=255)
                                                                                                                                                                                        aGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTc                                                          <  1:1093179/249‑1 (MQ=255)
                                                                                                                                                                                              tgctgcAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCtt                                                    >  1:617747/1‑249 (MQ=255)
                                                                                                                                                                                                 ngcAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTcc                                                 <  2:453903/248‑1 (MQ=255)
                                                                                                                                                                                                  gcAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCcg                                                <  1:763511/249‑1 (MQ=255)
                                                                                                                                                                                                   cAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCcgc                                               <  2:36371/249‑1 (MQ=255)
                                                                                                                                                                                                       nccAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGttt                                           <  2:570904/248‑1 (MQ=255)
                                                                                                                                                                                                       nccAGGAGAAGCAGAAGCCTCGTTCCATCTACGGCGTAATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGttt                                           <  2:1137701/248‑1 (MQ=255)
                                                                                                                                                                                                            gagaAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAg                                      <  2:251922/249‑1 (MQ=255)
                                                                                                                                                                                                             agaAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGt                                     <  2:6784/249‑1 (MQ=255)
                                                                                                                                                                                                                  cAGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGAc                                >  1:336728/1‑249 (MQ=255)
                                                                                                                                                                                                                   aGAAGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGAca                               >  1:1046129/1‑249 (MQ=255)
                                                                                                                                                                                                                      aGGCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGAcatca                            >  1:425310/1‑249 (MQ=255)
                                                                                                                                                                                                                        gCTCGTTTCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACatcatc                          <  2:978235/249‑1 (MQ=255)
                                                                                                                                                                                                                             tttCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGt                     <  2:1223377/249‑1 (MQ=255)
                                                                                                                                                                                                                              ttCATCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGtt                    >  2:1253718/1‑249 (MQ=255)
                                                                                                                                                                                                                                 aTCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGACCGGCAAGGCACTCGACGTCCCACCTTTCCGCGTTTCTCAGTCCGACATCATCAATGTTCGt                 >  2:832929/1‑249 (MQ=255)
                                                                                                                                                                                                                                 aTCTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGt                 <  1:433935/249‑1 (MQ=255)
                                                                                                                                                                                                                                   cTACGGCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTgn               >  2:167847/1‑248 (MQ=255)
                                                                                                                                                                                                                                       ggCGTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAg           >  1:1283003/1‑249 (MQ=255)
                                                                                                                                                                                                                                        gCGTCATGGAAGCGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGt          >  2:353605/1‑249 (MQ=255)
                                                                                                                                                                                                                                          gTCATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTcc        <  2:662315/249‑1 (MQ=255)
                                                                                                                                                                                                                                            cATGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTcccn      >  2:395747/1‑248 (MQ=255)
                                                                                                                                                                                                                                            cATCGAAACGCAGTTCCGGCGTTACCAGGTCGAGTCTAACCGTCGCGCAGGCAAGACCGGTGAGTACCTGGTCGTCCTGCTCGAGTGCCGTCTCGACAACGTTGTGGACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGGCGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCa      <  2:1157433/249‑1 (MQ=255)
                                                                                                                                                                                                                                             aTGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCT‑GCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCaga    <  2:753902/249‑1 (MQ=255)
                                                                                                                                                                                                                                              tGGAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCaga    <  1:445991/249‑1 (MQ=255)
                                                                                                                                                                                                                                                gAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCCCGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCagaag  <  1:858134/249‑1 (MQ=255)
                                                                                                                                                                                                                                                gAAACGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCagaag  <  2:555868/249‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
CCGTCCACCAAAGCGTCGTCGCGTTTAATAGGGAAGGAAAGGTAATACAAAATGGCTCGTTATACCGGCCCAGCAACCCGTAAATCCCGTCGTCTGCGCGTCGACCTTGTTGGTGGAGACATGGCGTTTGAGCGCCGTCCTTACCCTCCAGGACAGGCAGGCCGTGCACGCATCAAGGAGTCCGAGTACCTGCTGCAGCTCCAGGAGAAGCAGAAGGCTCGTTTCATCTACGGCGTCATGGAAAAGCAGTTCCGTCGTTACTACGCCGAGGCTAACCGTCGCGCAGGCAAGACCGGTGAGAACCTGGTCGTCCTGCTCGAGTCCCGCCTCGACAACGTCGTGTACCGCGCAGGTCTGGCAAACACCCGTCGCCAGGCTCGTCAGCTTGTTTCCCACGGTCACTTCACCGTGAACGGCAAGGCAATCGACGTTCCATCTTTCCGCGTTTCTCAGTACGACATCATCAATGTTCGTGAGAAGTCCCAGAAG  >  CP025533/576296‑576784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑