Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,843,580	+G	intergenic (‑1/+68)	pip ← / ← h16_B2516	prolyl aminopeptidase/transcriptional regulator, LysR‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,843,579	1	.	G	100.0%	123.0 / NA	32	M1M (ATG→CTG) †	pip	prolyl aminopeptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/14);  total (18/14)

GCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCTCCG  >  AM260480/2843461‑2843706                                                                                                                        |                                                                                                                                gccgGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACg                                                                                                                   <  1:730987/134‑1 (MQ=255)   cgGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGga                                                                                                                 <  2:502764/134‑1 (MQ=255)    ggCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                                                >  2:254693/1‑117 (MQ=255)    ggCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                                                <  1:254693/117‑1 (MQ=255)             ccGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGc                                                                                                       <  1:221004/134‑1 (MQ=255)                 gCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACg                                                                                                                   <  2:132146/118‑1 (MQ=255)                 gCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACg                                                                                                                   >  1:132146/1‑118 (MQ=255)                              cTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAg                                                                                                        >  2:762807/1‑116 (MQ=255)                              cTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAg                                                                                                        <  1:762807/116‑1 (MQ=255)                                    gtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACg                                                                                >  2:948959/1‑134 (MQ=255)                                    gtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACg                                                                                >  2:150650/1‑134 (MQ=255)                                             aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt                                                                       <  1:948959/134‑1 (MQ=255)                                             aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt                                                                       <  2:370227/134‑1 (MQ=255)                                                 ccGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAgcc                                                                   >  1:179747/1‑134 (MQ=255)                                                   gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGga                                                                                                                 >  1:758992/1‑86 (MQ=255)                                                   gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGga                                                                                                                 <  2:758992/86‑1 (MQ=255)                                                   gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                                                >  1:175699/1‑70 (MQ=255)                                                   gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                                                <  2:175699/70‑1 (MQ=255)                                                                     gCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAaca                                               >  2:789322/1‑134 (MQ=255)                                                                        ggTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                                         >  1:200801/1‑73 (MQ=255)                                                                        ggTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                                         <  2:200801/73‑1 (MQ=255)                                                                          tttCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGcc                                          >  1:326646/1‑134 (MQ=255)                                                                                        aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTc                                       >  1:149601/1‑123 (MQ=255)                                                                                        aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTc                                       <  2:149601/123‑1 (MQ=255)                                                                                          ttCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCAcc                                    <  2:453277/124‑1 (MQ=255)                                                                                          ttCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCAcc                                    >  1:453277/1‑124 (MQ=255)                                                                                          ttCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCt                            >  1:757962/1‑132 (MQ=255)                                                                                                  agagGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCg                                                                                         <  2:503950/63‑1 (MQ=255)                                                                                                  agagGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCg                                                                                         >  1:503950/1‑63 (MQ=255)                                                                                                   gagGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACcgcg                 <  2:757962/134‑1 (MQ=255)                                                                                                          cgcAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt                                                                       <  2:180314/73‑1 (MQ=255)                                                                                                          cgcAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt                                                                       >  1:180314/1‑73 (MQ=255)                                                                                                             aaGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACg       >  2:389319/1‑134 (MQ=255)                                                                                                              aGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGc      >  1:408947/1‑134 (MQ=255)                                                                                                               ggCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCt     >  1:739905/1‑134 (MQ=255)                                                                                                                  cGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCTCcg  >  1:527198/1‑134 (MQ=255)                                                                                                                        |                                                                                                                                GCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCTCCG  >  AM260480/2843461‑2843706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑