Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 637,111 +G intergenic (‑164/+218) adk ← / ← kdsB adenylate kinase/3‑Deoxy‑manno‑octulosonate cytidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260479637,1071.G100.0% 170.2 / NA 44intergenic (‑160/+222)adk/kdsBadenylate kinase/3‑Deoxy‑manno‑octulosonate cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (21/23);  total (21/23)

GGCGGGAGATCAGAAGGAGGGTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCA‑GGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGGGCGGCTCTGTATCGGCAAGCATTATGCCATGAGGCGG  >  AM260479/636978‑637232
                                                                                                                                  |                                                                                                                             
ggCGGGAGATCAGAAGGAGGGTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCA‑gggg                                                                                                                           >  1:542926/1‑134 (MQ=255)
             aaGGAGGGTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGgcggcgcgg                                                                                                               <  1:116403/134‑1 (MQ=255)
                  gggTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCacac                                                                                                          <  2:542926/134‑1 (MQ=255)
                   ggTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         <  2:444418/134‑1 (MQ=255)
                   ggTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         <  2:513687/134‑1 (MQ=255)
                        cGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACg                                                                                                    <  2:246329/134‑1 (MQ=255)
                           cGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGc                                                                                                 <  2:415280/134‑1 (MQ=255)
                           cGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGc                                                                                                 >  1:1037759/1‑134 (MQ=255)
                           cGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGc                                                                                                 >  1:94108/1‑134 (MQ=255)
                                 ttGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATc                                                                                           >  1:893066/1‑134 (MQ=255)
                                                        cTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         >  1:44699/1‑97 (MQ=255)
                                                        cTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         >  1:490722/1‑97 (MQ=255)
                                                        cTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         <  2:490722/97‑1 (MQ=255)
                                                        cTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         <  2:44699/97‑1 (MQ=255)
                                                         tGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACg                                                                                                    >  2:897833/1‑101 (MQ=255)
                                                         tGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACg                                                                                                    <  1:897833/101‑1 (MQ=255)
                                                           aTGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCg                                                                 <  1:862352/134‑1 (MQ=255)
                                                                    gACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCacac                                                                                                          >  1:637587/1‑84 (MQ=255)
                                                                    gACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCacac                                                                                                          <  2:637587/84‑1 (MQ=255)
                                                                    gACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         >  1:60660/1‑85 (MQ=255)
                                                                    gACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACAcc                                                                                                         <  2:60660/85‑1 (MQ=255)
                                                                    gACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGc                                                        >  1:667061/1‑134 (MQ=255)
                                                                    gACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGc                                                        >  2:597816/1‑134 (MQ=255)
                                                                      cTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCttt                                                                                        <  1:401640/100‑1 (MQ=255)
                                                                      cTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCttt                                                                                        >  2:401640/1‑100 (MQ=255)
                                                                         cGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATggcgg                                                                                                                    >  2:316149/1‑69 (MQ=255)
                                                                         cGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATggcgg                                                                                                                    <  1:316149/69‑1 (MQ=255)
                                                                             cAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATg                                               >  1:987380/1‑134 (MQ=255)
                                                                              aGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATgc                                              >  1:38642/1‑134 (MQ=255)
                                                                                  cgGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATgccggc                                          <  2:94108/134‑1 (MQ=255)
                                                                                   gggCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCacac                                                                                                          <  1:766885/69‑1 (MQ=255)
                                                                                   gggCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCacac                                                                                                          >  2:766885/1‑69 (MQ=255)
                                                                                       aCTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGggc                                     <  2:1037759/134‑1 (MQ=255)
                                                                                           cTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATgccggccgtatgcg                                  <  1:270023/133‑7 (MQ=255)
                                                                                              aGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGGGCGGctc                                <  2:987380/132‑1 (MQ=255)
                                                                                                         cGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATgccggc                                          >  1:489186/1‑111 (MQ=255)
                                                                                                         cGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATgccggc                                          <  2:489186/111‑1 (MQ=255)
                                                                                                         cGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGggc                                     >  2:755092/1‑116 (MQ=255)
                                                                                                         cGCATACGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGggc                                     <  1:755092/116‑1 (MQ=255)
                                                                                                              aCGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCt                                                                                          >  1:365010/1‑58 (MQ=255)
                                                                                                              aCGGCCGGCGGCGGCTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCt                                                                                          <  2:365010/58‑1 (MQ=39)
                                                                                                                    ggcggcggcTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGGGCGGCTCTGTATCGGCAAGCATTATGCCAt         >  1:355117/1‑133 (MQ=255)
                                                                                                                        gcggcTGCCAGGGGGATGGCGGCGCGGCACACCGGACg                                                                                                    <  2:544095/38‑1 (MQ=25)
                                                                                                                        gcggcTGCCAGGGGGATGGCGGCGCGGCACACCGGACg                                                                                                    >  1:544095/1‑38 (MQ=37)
                                                                                                                          ggcTGCCAGGGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCCCGCTTGCGCATGCCGGCCGGGCGGCTCTGTATCGGCAAGCATTATGCCATGAggcgg  >  2:481637/1‑134 (MQ=255)
                                                                                                                                  |                                                                                                                             
GGCGGGAGATCAGAAGGAGGGTAACGCCGTAACTTGACGCAGGACTTACTGCGGTCCTGATGCGTTCTGACTGCGTTCAGCGCGGGCACTGCTCAGGCGTACCCACGCATACGGCCGGCGGCGGCTGCCA‑GGGGATGGCGGCGCGGCACACCGGACGGGCTCTATCTTTATCTCCCCACCGAGCCTTGGCCGGAGGCACGCTTGCGCATGCCGGCCGGGCGGCTCTGTATCGGCAAGCATTATGCCATGAGGCGG  >  AM260479/636978‑637232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑