| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 2,616,505 | C→G | A45G (GCC→GGC) | h16_A2404 → | predicted esterase of the alpha‑beta hydrolase superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 2,616,505 | 0 | C | G | 95.0% | 69.1 / ‑3.0 | 20 | coding (134/921 nt) | h16_A2404 | predicted esterase of the alpha‑beta hydrolase superfamily |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (7/12); minor base . (0/1); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
CGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCG > AM260479/2616381‑2616630 | cgTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGgcggcgc > 2:561970/1‑134 (MQ=255) tCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGgcggcgcgg < 1:611430/134‑1 (MQ=255) ccTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc < 1:79907/133‑1 (MQ=255) ccTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc < 2:24945/133‑1 (MQ=255) cTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgcgg < 1:561970/134‑1 (MQ=255) ggTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCt < 2:548133/133‑1 (MQ=255) cgcgGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCata > 2:795803/1‑134 (MQ=255) gctgGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCCCCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc > 1:593260/1‑109 (MQ=255) gctgGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc < 2:593260/109‑1 (MQ=255) ctCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGa < 1:795803/132‑1 (MQ=255) ctCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGG‑CGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc < 2:5979/134‑1 (MQ=255) tCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc > 1:307433/1‑134 (MQ=255) gTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc < 2:307433/132‑1 (MQ=255) ccGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgcg > 1:398097/1‑88 (MQ=255) ccGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgcg < 2:398097/88‑1 (MQ=255) ccGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGc < 1:836543/133‑1 (MQ=255) ccGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGc < 1:8072/133‑1 (MQ=255) aGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc > 2:680877/1‑73 (MQ=255) aGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc < 1:680877/73‑1 (MQ=255) ggCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCg > 1:953858/1‑134 (MQ=255) | CGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCG > AM260479/2616381‑2616630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |