Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	628,642	Δ1 bp	coding (756/1206 nt)	h16_A0595 ←	Uncharacterized Fe‑S protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	628,641	0	A	.	100.0%	44.8 / NA	11	coding (757/1206 nt)	h16_A0595	Uncharacterized Fe‑S protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (3/8);  total (3/8)

CACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGG  >  AM260479/628513‑628753                                                                                                                                 |                                                                                                                  cacaTGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑Agcggc                                                                                                             >  1:334090/1‑134 (MQ=255) cacaTGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑Agcggc                                                                                                             >  1:456481/1‑134 (MQ=255) cacaTGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑Agcggc                                                                                                             >  1:521360/1‑134 (MQ=255) cacaTGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑Agcggc                                                                                                             >  1:816835/1‑134 (MQ=255) cacaTGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑Agcggc                                                                                                             >  2:227618/1‑134 (MQ=255)             cTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑ccagcc                                                                                                >  1:783205/1‑129 (MQ=255)             cTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑ccagcc                                                                                                <  1:907157/134‑6 (MQ=255)                gTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCg                                                                                                <  2:334090/132‑1 (MQ=255)                 ttCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGt                                                                                              <  2:816835/133‑1 (MQ=255)                          cAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGAAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCAc                                                                                    >  2:652966/1‑134 (MQ=255)                                      cAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGAAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGt                                                                        <  1:652966/134‑1 (MQ=255)                                        gtcgtcGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTgg                                                                      <  1:227618/134‑1 (MQ=255)                                                  ccGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTc                                                            <  2:783205/134‑1 (MQ=255)                                                          gcgGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGc                                                    <  2:196032/134‑1 (MQ=255)                                                                               gAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGc                               >  1:355852/1‑134 (MQ=255)                                                                                                             gATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCggg  <  2:355852/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                  CACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGG  >  AM260479/628513‑628753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑