Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	208,953	A→C	V315V (GTT→GTG)	h16_A0195 ←	Putative GTPase (G3E family)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	208,953	0	A	C	100.0%	77.1 / NA	21	V315V (GTT→GTG)	h16_A0195	Putative GTPase (G3E family)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/10);  total (11/10)

GGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGA  >  AM260479/208826‑209078                                                                                                                                |                                                                                                                              ggATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCg                                                                                                                         >  1:887257/1‑134 (MQ=255)  gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                                                                          <  1:749090/132‑1 (MQ=255)  gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                                                                          >  2:749090/1‑132 (MQ=255)           gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat                                                                                                                <  1:523914/133‑1 (MQ=255)           gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat                                                                                                                <  2:245750/133‑1 (MQ=255)                   ggTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGccc                                                                                                       >  1:819251/1‑134 (MQ=255)                                  acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGt                                                                                        >  2:915049/1‑134 (MQ=255)                                          cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                                                                          <  1:476007/92‑1 (MQ=255)                                          cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                                                                          >  2:476007/1‑92 (MQ=255)                                          cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTccc                                                                                 <  1:915049/133‑1 (MQ=255)                                                cccGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGtata                                                                           <  1:467968/133‑1 (MQ=255)                                                                             gATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                                                                       <  1:215041/60‑1 (MQ=255)                                                                             gATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                                                                       >  2:215041/1‑60 (MQ=255)                                                                                             aGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGtata                                                                           <  1:678404/88‑1 (MQ=255)                                                                                             aGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGtata                                                                           >  2:678404/1‑88 (MQ=255)                                                                                                             ggAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCg              >  1:368017/1‑133 (MQ=255)                                                                                                                 caccacTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGc                                                               <  2:695235/80‑1 (MQ=255)                                                                                                                 caccacTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGc                                                               >  1:695235/1‑80 (MQ=255)                                                                                                                        ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAg                                                                    <  1:935898/68‑1 (MQ=255)                                                                                                                        ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAg                                                                    >  2:935898/1‑68 (MQ=255)                                                                                                                        ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGacgaacga  >  2:354040/1‑134 (MQ=255)                                                                                                                                |                                                                                                                              GGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGA  >  AM260479/208826‑209078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑