| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 2,580,039 | A→G | R155R (CGT→CGC) | h16_A2375 ← | Protein‑L‑isoaspartate carboxylmethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 2,580,039 | 0 | A | G | 100.0% | 35.9 / NA | 11 | R155R (CGT→CGC) | h16_A2375 | Protein‑L‑isoaspartate carboxylmethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/5); total (6/5) | |||||||||||
CGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGG > AM260479/2579920‑2580159 | cggATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt < 2:43848/134‑1 (MQ=255) gATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCa > 1:375282/1‑134 (MQ=255) gaCCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACg > 1:1029321/1‑134 (MQ=255) ccTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCgg > 1:158719/1‑133 (MQ=255) gTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAg < 2:923640/132‑1 (MQ=255) tAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAgcgc < 2:1029321/134‑1 (MQ=255) ccGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCt > 1:992382/1‑133 (MQ=255) ccGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCt > 2:475198/1‑133 (MQ=255) gAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCg < 1:475198/131‑1 (MQ=255) cTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCg < 2:375282/134‑1 (MQ=255) cggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTgg > 1:813852/1‑134 (MQ=255) | CGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGG > AM260479/2579920‑2580159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |