| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 3,515,314 | Δ1 bp | intergenic (+253/‑570) | tRNA‑Pro (CGG) → / → pilB | tRNA‑Pro/Type IV pilus assembly protein PilB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 3,515,314 | 0 | G | . | 100.0% | 127.3 / NA | 29 | intergenic (+253/‑570) | tRNA‑Pro (CGG)/pilB | tRNA‑Pro/Type IV pilus assembly protein PilB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (17/12); total (17/12) | |||||||||||
GCTGGCTACGACCCACTACTGCATGGGGCGCGAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCTT > AM260479/3515199‑3515430 | gCTGGCTACGACCCACTACTGCATGGGGCGCGAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAg < 2:1023322/134‑1 (MQ=255) ctGCATGGGGCGCGAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAg > 2:921613/1‑134 (MQ=255) cATGGGGCGCGAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTc < 1:921613/133‑1 (MQ=255) cgcgAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCt > 1:916534/1‑134 (MQ=255) gcgAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCtt < 1:933135/134‑1 (MQ=255) cgAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGt > 1:743809/1‑96 (MQ=255) cgAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCtt > 2:800198/1‑133 (MQ=255) cgAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCtt > 2:933135/1‑133 (MQ=255) cgAGCTTGCCGTCGCGCACCAGCCGCCGGAAGCTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGt < 2:743809/96‑1 (MQ=255) aGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTTGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGa > 1:111876/1‑133 (MQ=255) aGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCcg > 1:679850/1‑88 (MQ=255) aGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCcg < 2:679850/88‑1 (MQ=255) aGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGa > 2:196404/1‑133 (MQ=255) gTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCcgcg < 1:65537/82‑1 (MQ=255) gTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCcgcg > 2:65537/1‑82 (MQ=255) ccgGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTgg < 1:679820/73‑1 (MQ=255) ccgGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTgg > 2:679820/1‑73 (MQ=255) ccgGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTcc < 1:880550/100‑1 (MQ=255) ccgGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTcc > 2:880550/1‑100 (MQ=255) cTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACgg > 2:1040932/1‑134 (MQ=255) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTg > 1:300074/1‑48 (MQ=39) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTg < 2:300074/48‑1 (MQ=39) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGc < 2:965653/49‑1 (MQ=39) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGc > 1:965653/1‑49 (MQ=39) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACtt > 2:1040113/1‑99 (MQ=255) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACtt < 1:1040113/99‑1 (MQ=255) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGa < 2:833293/128‑1 (MQ=255) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGa > 1:833293/1‑128 (MQ=255) cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGcct > 2:790619/1‑132 (MQ=255) | GCTGGCTACGACCCACTACTGCATGGGGCGCGAGCTTGCCGTCGCGCACCAGCCGCCGGAAGGTGGCAATTGAAACTTCGAGGTATTCGGCCGCCTCCGCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCTT > AM260479/3515199‑3515430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |