Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,431,093	Δ1 bp	coding (2380/2469 nt)	h16_B2148 →	membrane carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,431,092	0	T	.	100.0%	81.8 / NA	19	coding (2379/2469 nt)	h16_B2148	membrane carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/10);  total (9/10)

GGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCC  >  AM260480/2430966‑2431218                                                                                                                               |                                                                                                                               ggCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCgggggg                                                                                                                        <  2:342084/134‑1 (MQ=255)            cGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGAtt                                                                                                             >  2:1008213/1‑134 (MQ=255)                      tCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGAtcggg                                                                                                                            <  2:832614/110‑5 (MQ=255)                      tCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGAtcggg                                                                                                                            >  1:832614/1‑106 (MQ=255)                        ggccggcGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAg                                                                                                 <  1:1155341/134‑1 (MQ=255)                        ggccggcGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAg                                                                                                 >  1:173061/1‑134 (MQ=255)                        ggccggcGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAg                                                                                                 >  1:956527/1‑134 (MQ=255)                                                 cGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAgcg                                                                                       >  2:763157/1‑119 (MQ=255)                                                 cGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAgcg                                                                                       <  1:763157/119‑1 (MQ=255)                                                      tCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGgag                                                                                                                      <  1:1151914/83‑1 (MQ=255)                                                      tCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGgag                                                                                                                      >  2:1151914/1‑83 (MQ=255)                                                                       gcgGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGa                                                  <  2:956527/134‑1 (MQ=255)                                                                       gcgGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGa                                                  <  2:606220/134‑1 (MQ=255)                                                                       gcgGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGa                                                  <  2:173061/134‑1 (MQ=255)                                                                                           ggATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGa                                                  <  2:1074628/114‑1 (MQ=255)                                                                                           ggATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGa                                                  >  1:1074628/1‑114 (MQ=255)                                                                                                tCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTgc                                                                    <  1:88893/91‑1 (MQ=255)                                                                                                tCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTgc                                                                    >  2:88893/1‑91 (MQ=255)                                                                                                          tGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGa                                                                                               <  2:1076710/54‑1 (MQ=255)                                                                                                          tGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGa                                                                                               >  1:1076710/1‑54 (MQ=255)                                                                                                                       gcgcTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATccgcc  >  1:308823/1‑134 (MQ=255)                                                                                                                               |                                                                                                                               GGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCC  >  AM260480/2430966‑2431218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑