Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,615,722	+A	intergenic (+48/‑141)	prfA → / → h16_A3341	protein chain release factor A (RF‑1)/Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,615,720	1	.	A	100.0%	131.8 / NA	35	intergenic (+46/‑143)	prfA/h16_A3341	protein chain release factor A (RF‑1)/Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (16/19);  total (16/19)

GCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTA  >  AM260479/3615595‑3615830                                                                                                                                |                                                                                                               gCGACATGGATGAGCTGCTGTCGGTACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGT‑aaagtcg                                                                                                         <  1:25643/134‑6 (MQ=255) gCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGT‑aaagtcg                                                                                                         <  2:203977/134‑6 (MQ=255)    aCATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCg                                                                                                       <  2:414311/134‑1 (MQ=255)      aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGa                                                                                                      >  2:1118944/1‑133 (MQ=255)      aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGa                                                                                                      >  1:127137/1‑133 (MQ=255)            gAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCttt                                                                                               >  2:175534/1‑134 (MQ=255)                   tgTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGc                                                                                                         <  2:521870/117‑1 (MQ=255)                   tgTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGc                                                                                                         >  1:521870/1‑117 (MQ=255)                     tCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCtt                                                                                      <  2:131493/134‑1 (MQ=255)                          aCTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa                                                                                 <  1:1118944/134‑1 (MQ=255)                          aCTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa                                                                                 <  1:175534/134‑1 (MQ=255)                                  cGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTg                                                                                              <  2:842630/113‑1 (MQ=255)                                  cGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTg                                                                                              >  1:842630/1‑113 (MQ=255)                                              cTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTgg                                                               >  1:977647/1‑132 (MQ=255)                                                    aGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGt                                                        <  2:127137/133‑1 (MQ=255)                                                                          gTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGAtt                                 <  1:190583/134‑1 (MQ=255)                                                                           tCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa                                                                                 >  1:169311/1‑85 (MQ=255)                                                                           tCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa                                                                                 <  2:169311/85‑1 (MQ=255)                                                                                 gCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt                                                                            >  2:141556/1‑84 (MQ=255)                                                                                 gCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt                                                                            <  1:1048412/84‑1 (MQ=255)                                                                                 gCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt                                                                            >  2:1048412/1‑84 (MQ=255)                                                                                 gCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt                                                                            <  1:141556/84‑1 (MQ=255)                                                                                 gCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCg                          <  2:380684/134‑1 (MQ=255)                                                                                 gCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCg                          <  2:977647/134‑1 (MQ=255)                                                                                                gTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCa                       >  1:418345/1‑122 (MQ=255)                                                                                                gTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCa                       <  2:418345/122‑1 (MQ=255)                                                                                                   cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGc                                                                                                         <  1:861926/37‑1 (MQ=255)                                                                                                   cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGc                                                                                                         >  2:861926/1‑37 (MQ=255)                                                                                                   cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTa                                                                                            <  1:595523/50‑1 (MQ=255)                                                                                                   cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTa                                                                                            >  2:595523/1‑50 (MQ=255)                                                                                                   cAACCGGCCTTCCGCCCAGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGc        >  1:626239/1‑134 (MQ=255)                                                                                                     aCCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAggcgg                                                 <  1:670040/91‑1 (MQ=255)                                                                                                     aCCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAggcgg                                                 >  2:670040/1‑91 (MQ=255)                                                                                                     aCCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGAc                            >  2:1108753/1‑112 (MQ=255)                                                                                                     aCCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGAc                            <  1:1108753/112‑1 (MQ=255)                                                                                                     aCCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCg      >  1:1025081/1‑134 (MQ=255)                                                                                                         gCCTTCCGCCCAGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTa  <  2:626239/134‑1 (MQ=255)                                                                                                                                |                                                                                                               GCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTA  >  AM260479/3615595‑3615830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑