Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	157,096	+G	intergenic (‑26/+41)	h16_B0136 ← / ← h16_B0137	cysteine desulfurase/conserved hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	157,094	1	.	G	100.0%	122.9 / NA	32	intergenic (‑24/+43)	h16_B0136/h16_B0137	cysteine desulfurase/conserved hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/15);  total (17/15)

CACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCTCAT  >  AM260480/156969‑157217                                                                                                                               |                                                                                                                            cACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGAcgcg                                                                                                                      >  2:463072/1‑134 (MQ=255) cACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGAcgcg                                                                                                                      >  1:896213/1‑134 (MQ=255)     gcgTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGa                                                                                                                  >  2:125986/1‑134 (MQ=255)        tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg                                                                                                               >  2:404292/1‑134 (MQ=255)        tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg                                                                                                               >  2:280381/1‑134 (MQ=255)          gggCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              <  1:125986/133‑1 (MQ=255)          gggCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              <  1:463072/133‑1 (MQ=255)          gggCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              <  1:280381/133‑1 (MQ=255)                   cGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGt                                                                                                    <  1:404292/134‑1 (MQ=255)                          gcggcgGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTc                                                                                             <  2:896213/134‑1 (MQ=255)                                           gatGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttactt                                                                             <  1:642887/133‑1 (MQ=255)                                               gtggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              <  2:498862/96‑1 (MQ=255)                                               gtggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              >  1:498862/1‑96 (MQ=255)                                               gtggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGcccc                                                                                                         <  1:347511/101‑1 (MQ=255)                                               gtggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGcccc                                                                                                         >  2:347511/1‑101 (MQ=255)                                                 ggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGc                                                                      >  1:276646/1‑134 (MQ=255)                                                    ggCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttactt                                                                             <  1:856380/124‑1 (MQ=255)                                                    ggCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttactt                                                                             >  2:856380/1‑124 (MQ=255)                                                     gCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTgg                                                                  >  1:1401/1‑134 (MQ=255)                                                                       gcgcGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGc                                                <  2:1401/134‑1 (MQ=255)                                                                              tCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTg                                                                   <  2:255979/108‑1 (MQ=255)                                                                              tCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTg                                                                   >  1:255979/1‑108 (MQ=255)                                                                                              gATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAAt                          >  2:191471/1‑133 (MQ=255)                                                                                               atatCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCa                        >  2:798889/1‑134 (MQ=255)                                                                                                  tCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCAcgc                     <  1:798889/134‑1 (MQ=255)                                                                                                   ccATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCAcgcc                    >  1:982107/1‑134 (MQ=255)                                                                                                        gAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              >  1:992774/1‑39 (MQ=37)                                                                                                        gAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                              <  2:992774/39‑1 (MQ=37)                                                                                                         aaCGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGc              <  1:823779/134‑1 (MQ=255)                                                                                                                 gggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGgcgc      >  1:1149374/1‑134 (MQ=255)                                                                                                                 gggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGgcgc      >  1:674073/1‑134 (MQ=255)                                                                                                                      aTGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCTCAt  <  2:276646/133‑1 (MQ=255)                                                                                                                               |                                                                                                                            CACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCTCAT  >  AM260480/156969‑157217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑