Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 507,425 +A coding (27/564 nt) h16_B0448 → fragment of ABC‑type transport system,periplasmic component: APC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480507,4231.A100.0% 152.6 / NA 41E9K (GAA→AAA) h16_B0448fragment of ABC‑type transport system,periplasmic component: APC family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (23/18);  total (23/18)

GTAGGGCAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATG‑AACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAT  >  AM260480/507305‑507549
                                                                                                                       |                                                                                                                              
gTAGGGCAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTg                                                                                                                  >  1:1188157/1‑134 (MQ=255)
       aaTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                           >  2:258674/1‑134 (MQ=255)
        aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcgc                                                                                                          >  2:143134/1‑134 (MQ=255)
          aCCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcgccg                                                                                                        >  1:492149/1‑134 (MQ=255)
             aCGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcgccgccg                                                                                                     >  2:269456/1‑134 (MQ=255)
                  cggcgTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctg                                                                                                >  1:1094676/1‑134 (MQ=255)
                  cggcgTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctg                                                                                                >  1:1116960/1‑134 (MQ=255)
                  cggcgTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctg                                                                                                >  1:504419/1‑134 (MQ=255)
                      gTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctggcgc                                                                                            <  1:258674/134‑1 (MQ=255)
                           ccgccTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                       <  2:492149/134‑1 (MQ=255)
                           ccgccTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                       <  1:283462/134‑1 (MQ=255)
                                  aCATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGcc                                                                                  <  2:1116960/132‑1 (MQ=255)
                                  aCATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGcc                                                                                  <  2:1094676/132‑1 (MQ=255)
                                     tGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             <  2:504419/134‑1 (MQ=255)
                                     tGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             <  2:1188157/134‑1 (MQ=255)
                                      ggggAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCt                                                                            >  2:124659/1‑134 (MQ=255)
                                       gggAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCt                                                                            <  1:269456/133‑1 (MQ=255)
                                             cGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGt                                                                     <  1:143134/134‑1 (MQ=255)
                                                tCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCt                                                                  >  1:1112896/1‑134 (MQ=255)
                                                             cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                       >  1:962630/1‑100 (MQ=255)
                                                             cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                       <  2:962630/100‑1 (MQ=255)
                                                             cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATG‑aaac                                                                                                                            >  1:691283/1‑60 (MQ=255)
                                                             cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATG‑aaac                                                                                                                            <  2:691283/62‑3 (MQ=255)
                                                                  gggCACCACATAGAGTGCCTGACGGGCAATGACCATCCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCAtt                                                >  2:990345/1‑134 (MQ=255)
                                                                                  gCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                           >  1:668329/1‑59 (MQ=255)
                                                                                  gCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                           <  2:668329/59‑1 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             <  2:1004190/85‑1 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             >  1:1004190/1‑85 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCt                                              >  2:1045298/1‑116 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCt                                              <  1:1045298/116‑1 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGcc                            >  1:735945/1‑134 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGcc                            >  2:290391/1‑134 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGcc                            >  1:325942/1‑134 (MQ=255)
                                                                                      gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGcc                            >  1:1117419/1‑134 (MQ=255)
                                                                                         gggCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTc                         >  2:419977/1‑134 (MQ=255)
                                                                                           gCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             >  2:174716/1‑80 (MQ=255)
                                                                                           gCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             <  1:174716/80‑1 (MQ=255)
                                                                                               tGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             >  2:1058226/1‑76 (MQ=255)
                                                                                               tGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                             <  1:1058226/76‑1 (MQ=255)
                                                                                                   aaTTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTc               <  2:735945/134‑1 (MQ=255)
                                                                                                         aCGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctggcgc                                                                                            >  1:513189/1‑51 (MQ=38)
                                                                                                         aCGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctggcgc                                                                                            <  2:513189/51‑1 (MQ=38)
                                                                                                                agaCATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAt  <  2:1117419/134‑1 (MQ=255)
                                                                                                                       |                                                                                                                              
GTAGGGCAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATG‑AACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAT  >  AM260480/507305‑507549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑