Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	951,073	+G	intergenic (‑29/+230)	h16_B0838 ← / ← h16_B0839	conserved hypothetical protein/transcriptional regulator, CRP‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	951,073	1	.	G	100.0%	86.8 / NA	23	intergenic (‑29/+230)	h16_B0838/h16_B0839	conserved hypothetical protein/transcriptional regulator, CRP‑family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/12);  total (11/12)

CGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCAC  >  AM260480/950958‑951187                                                                                                                     |                                                                                                                   cgcTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAg                                                                                                        <  1:1062491/129‑1 (MQ=255) cgcTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAg                                                                                                        >  2:1062491/1‑129 (MQ=255)                              gggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGAc                                                                       >  1:1181445/1‑133 (MQ=255)                               ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGAc                                                                       >  2:697793/1‑132 (MQ=255)                                                          catcggctgcatcggctgCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTc                                          <  1:449237/134‑1 (MQ=255)                                                           atcggctgcatcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCTATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCg                                         >  2:56516/1‑134 (MQ=255)                                                            tcggctgcatcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCCCGACCAGGGATTTCGCCAGATGTTGCTTCATTCgt                                        >  2:210509/1‑134 (MQ=255)                                                                     tcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcct                               <  1:697793/134‑1 (MQ=255)                                                                            cGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACg                         <  1:486892/133‑1 (MQ=255)                                                                             gTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGcc                       >  2:70433/1‑134 (MQ=255)                                                                                aTTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGc                        <  2:834401/130‑1 (MQ=255)                                                                                aTTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGc                        >  1:834401/1‑130 (MQ=255)                                                                                 ttGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACa                   <  2:1181445/134‑1 (MQ=255)                                                                                     cATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCa               <  1:961643/134‑1 (MQ=255)                                                                                      aTCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGc                >  2:777300/1‑132 (MQ=255)                                                                                      aTCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCaa              <  1:70433/134‑1 (MQ=255)                                                                                      aTCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCaa              <  1:210509/134‑1 (MQ=255)                                                                                          gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCAtt                                           >  1:712489/1‑101 (MQ=255)                                                                                          gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCAtt                                           <  2:712489/101‑1 (MQ=255)                                                                                           ctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGaa         <  1:977283/134‑1 (MQ=255)                                                                                            tggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGAt                                                      <  2:521304/88‑1 (MQ=255)                                                                                            tggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGAt                                                      >  1:521304/1‑88 (MQ=255)                                                                                                  ggCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCAc  >  2:76962/1‑134 (MQ=255)                                                                                                                     |                                                                                                                   CGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCAC  >  AM260480/950958‑951187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑