Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	547,818	+C	intergenic (‑10/+27)	glyS ← / ← h16_A0522	Glycyl‑tRNA synthetase beta subunit/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	547,817	1	.	C	100.0%	117.5 / NA	31	intergenic (‑9/+28)	glyS/h16_A0522	Glycyl‑tRNA synthetase beta subunit/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (14/17);  total (14/17)

AGGCCGGCGAACAGGCCCTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTAT‑CAACAGGTTGTAGCGCGCGCCCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGAGCAGCAGCCACAGGCCGTTCTGTTGCCGGAATTGCAGCGT  >  AM260479/547696‑547919                                                                                                                           |                                                                                                       aGGCCGGCGAACAGGCCCTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGt                                                                                             <  2:621069/134‑1 (MQ=255)       gCGAACAGGCCCTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAgcgcg                                                                                       >  2:562825/1‑134 (MQ=255)        cGAACAGGCCCTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAgcgcgc                                                                                      <  1:187381/134‑1 (MQ=255)        cGAACAGGCCCTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAgcgcgc                                                                                      <  1:773385/134‑1 (MQ=255)                 ccTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGt                                                                            >  2:305273/1‑134 (MQ=255)                           ggCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGcgcg                                                                  <  1:562825/134‑1 (MQ=255)                             cGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCgcggc                                                                >  1:339313/1‑134 (MQ=255)                                 gCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGaaa                                                            <  1:305273/134‑1 (MQ=255)                                   ccAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGa                                                              >  2:554480/1‑130 (MQ=255)                                                        gTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCggacagc                                     <  2:322582/134‑7 (MQ=255)                                                                         aaCAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTg                    <  2:751709/134‑1 (MQ=255)                                                                             gTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACt                                              >  1:1030137/1‑104 (MQ=255)                                                                             gTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACt                                              <  2:1030137/104‑1 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCgg                                          <  2:885178/103‑2 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCgg                                          >  1:885178/1‑102 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGc                           <  2:197077/118‑1 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGc                           >  1:197077/1‑118 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGc                           >  1:372238/1‑118 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGc                           <  2:372238/118‑1 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTGTTGCCGGaa           >  1:709022/1‑134 (MQ=255)                                                                                  aTCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTGTTGCCGGaa           >  1:599297/1‑134 (MQ=255)                                                                                     agcagcGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCg                         >  1:1017672/1‑117 (MQ=255)                                                                                     agcagcGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCg                         <  2:1017672/117‑1 (MQ=255)                                                                                      gcagcGAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTGTTGCCGGAATTGc       <  2:339313/134‑1 (MQ=255)                                                                                           gAGTCGGTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTGTTGCCGGAATTGCAGCgt  <  2:599297/134‑1 (MQ=255)                                                                                                ggTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTGTTGCCGGAATTg        >  2:431692/1‑123 (MQ=255)                                                                                                ggTCATGGGTTGCGACATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCCACAGGCCGTTCTGTTGCCGGAATTg        <  1:431692/123‑1 (MQ=255)                                                                                                               cATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCca                                <  1:687458/84‑1 (MQ=255)                                                                                                               cATGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGGACAGCAGCca                                >  2:687458/1‑84 (MQ=255)                                                                                                                aTGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAgcgc                                                                   >  1:288449/1‑48 (MQ=255)                                                                                                                aTGAATCGTATCCAACAGGTTGTAGCGCGCG‑CCGCTTGTCAGCAgcgc                                                                   <  2:288449/48‑1 (MQ=37)                                                                                                                           |                                                                                                       AGGCCGGCGAACAGGCCCTGCGCGAAGGCGTCGCCCAGGCGCGCCAGCGCCTTGGGTGGCAGCTCTTCGGTGAACAGTTCGATCAGCAGCGAGTCGGTCATGGGTTGCGACATGAATCGTAT‑CAACAGGTTGTAGCGCGCGCCCGCTTGTCAGCAGCGCGGCGAAAGATTAGGTTCCACTCCGAGCAGCAGCCACAGGCCGTTCTGTTGCCGGAATTGCAGCGT  >  AM260479/547696‑547919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑