| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 724,768 | +C | coding (82/669 nt) | lysE → | lysine efflux permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 724,766 | 1 | . | C | 100.0% | 117.8 / NA | 31 | R27P (CGC→CCC) | lysE | lysine efflux permease |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (14/17); total (14/17) | |||||||||||
AGGTAGTCCTGCGGCAGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCG‑CCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTCTGGGTCTGCCTGGTGTG > AM260479/724660‑724880 | aGGTAGTCCTGCGGCAGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGcc > 1:196480/1‑115 (MQ=255)aGGTAGTCCTGCGGCAGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGcc < 2:196480/115‑1 (MQ=255) gTAGTCCTGCGGCAGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTcc > 2:644714/1‑134 (MQ=255) aGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTtct > 2:758233/1‑107 (MQ=255) aGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTtct < 1:758233/107‑1 (MQ=255) gcgggcATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGc > 1:1034659/1‑134 (MQ=255) cgggcATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCAt > 2:568867/1‑118 (MQ=255) cgggcATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCAt < 1:568867/118‑1 (MQ=255) cggcTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGtgctg < 2:1093529/134‑1 (MQ=255) ataataGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGcgcg < 2:378247/134‑1 (MQ=255) ccACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCAtgt > 1:268903/1‑134 (MQ=255) ccACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCAtgt < 1:644714/134‑1 (MQ=255) cACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGcgcg < 1:1115315/126‑1 (MQ=255) cACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGcgcg > 2:1115315/1‑126 (MQ=255) cccAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTg > 1:940423/1‑117 (MQ=255) cccAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTg < 2:940423/117‑1 (MQ=255) ccAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTc < 2:191690/134‑1 (MQ=255) ccAAAAAAACAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTc < 2:23446/134‑1 (MQ=255) aaaaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTc < 2:48584/54‑1 (MQ=39) aaaaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTc > 1:48584/1‑54 (MQ=39) aaaaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCg < 2:859294/55‑1 (MQ=255) aaaaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCg > 1:859294/1‑55 (MQ=255) aaaaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGa < 2:252566/122‑1 (MQ=255) aaaaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGa > 1:252566/1‑122 (MQ=255) aaaaaCAGCCGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTtct > 1:972935/1‑47 (MQ=38) aaaaaCAGCAGTTCCACATCCAATGCACGCCGCCCTGGCCGGCTtct < 2:972935/47‑1 (MQ=37) gTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTc > 1:738560/1‑120 (MQ=255) gTTCCACATCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTc < 2:738560/120‑1 (MQ=255) cacaTCCAATGCACGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTCTGGGTCTGCCTGGtgtg < 2:620054/133‑1 (MQ=255) aCGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGgcgcta > 2:431357/1‑92 (MQ=255) aCGCCGCCCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGgcgcta < 1:431357/94‑3 (MQ=255) | AGGTAGTCCTGCGGCAGGAATGGGAAGATGGGCGGGCATGGCGGCTGCCCCCGGCGTGCAATAATAGCCACCCAAAAAAACAGCCGTTCCACATCCAATGCACGCCG‑CCTGGCCGGCTTCTCCCTCGGTCTTTCCCTGATTCTTGCCATCGGTTCCCAGAATGCTTTCGTGCTGCGACAAGGGTTGCGGCGCGAGCATGTGTTCTGGGTCTGCCTGGTGTG > AM260479/724660‑724880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |