| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 1,081,436 | +C | coding (437/447 nt) | h16_A0989 → | Hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 1,081,433 | 1 | . | C | 94.1% | 55.9 / ‑2.6 | 17 | coding (434/447 nt) | h16_A0989 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (8/8); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGAGCTGGTGATTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑C‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCC > AM260479/1081304‑1081508 | aGGAGCTGGTGATTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c‑ccc < 1:508221/134‑2 (MQ=255) cTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c‑ccc > 1:300101/1‑116 (MQ=255) cTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c‑ccc < 2:300101/117‑2 (MQ=255) cgtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCgggg > 2:817744/1‑133 (MQ=255) cgtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCgggg > 2:703210/1‑133 (MQ=255) cgtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGc < 2:654/134‑1 (MQ=255) gtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c‑ccc > 1:1100312/1‑98 (MQ=255) gtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c‑ccc < 2:1100312/99‑2 (MQ=255) tcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCGATGGGGGCCATGCCGGCGTCGAAGCCTCGGTCGGCGGCACGCAAGG‑CACCTGGACCGTGATCTACGATGTGGACCTGCCGGGGCAg < 2:75383/134‑1 (MQ=255) cGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACg < 2:687667/134‑1 (MQ=255) gcgcTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTAc > 2:771762/1‑133 (MQ=255) cTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATa > 2:795706/1‑134 (MQ=255) aTGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGa < 1:281895/106‑1 (MQ=255) aTGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGa > 2:281895/1‑106 (MQ=255) cGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGc < 1:795706/134‑1 (MQ=255) gggCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGccc < 1:771762/134‑1 (MQ=255) aTGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGa > 1:869893/1‑97 (MQ=255) aTGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGa < 2:869893/97‑1 (MQ=255) ccATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAgg > 2:554668/1‑39 (MQ=255) ccATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAgg < 1:554668/39‑1 (MQ=255) tGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c > 1:951573/1‑37 (MQ=255) tGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑c < 2:951573/37‑1 (MQ=255) cccGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTAGGATACCCGCGTGCCGCTGGCCCAGcc < 1:806506/109‑1 (MQ=255) cccGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTAGGATACCCGCGTGCCGCTGGCCCAGcc > 2:806506/1‑109 (MQ=255) gAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCggg > 2:415975/1‑62 (MQ=255) gAAGCCTCGGCCGGCGGCACGCAAGGCC‑CCTGGACCGTGATCTACGATGCGGACCTGCCggg < 1:415975/62‑1 (MQ=255) | AGGAGCTGGTGATTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑C‑CCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCC > AM260479/1081304‑1081508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |