| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 1,354,645 | +C | intergenic (+98/‑15) | h16_A1248 → / → h16_A1249 | predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 1,354,643 | 1 | . | C | 94.3% | 125.6 / 0.5 | 35 | intergenic (+96/‑17) | h16_A1248/h16_A1249 | predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (18/15); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
ATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAA > AM260479/1354536‑1354760 | aTGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGcc < 2:779806/134‑1 (MQ=255) gTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACttt < 1:1021043/97‑1 (MQ=255) gTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACttt > 2:1021043/1‑97 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAAt > 2:406756/1‑117 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAAt < 1:406756/117‑1 (MQ=255) aGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAg < 2:632810/77‑1 (MQ=255) aGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAg > 1:632810/1‑77 (MQ=255) aGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGc > 1:1013270/1‑108 (MQ=255) aGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGc < 2:1013270/108‑1 (MQ=255) aTCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCgg > 2:158136/1‑134 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATgcgc < 2:1109641/89‑1 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATgcgc > 1:1109641/1‑89 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt > 1:1101723/1‑108 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt < 2:1101723/108‑1 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑ccc < 1:807784/41‑2 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑ccc > 2:807784/1‑40 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAg > 1:924224/1‑57 (MQ=39) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAg < 2:924224/57‑1 (MQ=39) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCg < 1:535914/87‑1 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCg > 2:535914/1‑87 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt > 1:600244/1‑98 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt < 2:600244/98‑1 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAg > 1:101953/1‑105 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAg < 2:101953/105‑1 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTc > 1:254326/1‑132 (MQ=255) tCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAg < 2:240281/50‑1 (MQ=38) tCATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAg > 1:240281/1‑50 (MQ=38) cATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAg < 1:949392/61‑1 (MQ=255) cATGTGGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAg > 2:949392/1‑61 (MQ=255) tgtgGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCGGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 2:660544/1‑133 (MQ=255) tgtgGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 1:676871/1‑133 (MQ=255) tgtgGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 1:360835/1‑133 (MQ=255) tgGCAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGaa < 2:360835/134‑1 (MQ=255) cAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGgcggcg > 2:1146727/1‑129 (MQ=255) cAGGCCATCGACGCCC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGgcggcg < 1:1146727/129‑1 (MQ=255) gCCATCGACG‑CCATGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTAcc > 1:835331/1‑103 (MQ=255) gCCATCGACG‑CCATGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTAcc < 2:835331/103‑1 (MQ=255) | ATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CC‑TGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAA > AM260479/1354536‑1354760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |