Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,111,023	+G	coding (563/1089 nt)	h16_A1942 →	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,111,022	1	.	G	100.0%	62.7 / NA	17	R188G (AGA→GGA)	h16_A1942	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/10);  total (7/10)

GCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGT  >  AM260479/2110899‑2111132                                                                                                                              |                                                                                                               gCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGAt                                                                                                        >  2:682053/1‑134 (MQ=255)  ccAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc                                                                                                       >  2:161468/1‑134 (MQ=255)           ttCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGAcgccgcc                                                                                              <  1:930703/134‑1 (MQ=255)           ttCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGAcgccgcc                                                                                              <  2:795894/134‑1 (MQ=255)                          cgcAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGcc                                                                               <  1:161468/134‑1 (MQ=255)                                      ggCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTc                                                                   <  1:571010/134‑1 (MQ=255)                                                cAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGccc                                                         >  2:408141/1‑134 (MQ=255)                                                            gcgcATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccgccg                                                              <  1:749328/117‑1 (MQ=255)                                                            gcgcATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccgccg                                                              >  2:749328/1‑117 (MQ=255)                                                                      gCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGcgcgccg                                    <  1:470498/133‑1 (MQ=255)                                                                          aCGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGcgccc                               >  2:307477/1‑134 (MQ=255)                                                                                                   cgacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcgc       <  1:408141/133‑1 (MQ=255)                                                                                                     acgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcg        >  2:162496/1‑130 (MQ=255)                                                                                                     acgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcg        <  1:162496/130‑1 (MQ=255)                                                                                                      cgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCgcggcg   >  2:473899/1‑134 (MQ=255)                                                                                                       gaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCgcggcgc  <  1:473899/134‑2 (MQ=255)                                                                                                       gaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGt  <  1:307477/134‑1 (MQ=255)                                                                                                                              |                                                                                                               GCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGT  >  AM260479/2110899‑2111132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑