| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 3,302,920 | +C | coding (1199/1251 nt) | dfp → | phosphopantothenoylcysteine synthetase/decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 3,302,918 | 1 | . | C | 94.1% | 57.3 / ‑2.9 | 17 | coding (1197/1251 nt) | dfp | phosphopantothenoylcysteine synthetase/decarboxylase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (7/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
GGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCC > AM260479/3302795‑3303046 | ggACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATggc > 2:914052/1‑134 (MQ=255) aaCGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGgcgcg < 2:965383/134‑1 (MQ=255) cTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCa < 2:53624/134‑1 (MQ=255) gTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATg > 2:339349/1‑134 (MQ=255) gACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc < 1:914052/133‑1 (MQ=255) gACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc < 1:339349/133‑1 (MQ=255) aCGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCgg > 2:593337/1‑134 (MQ=255) cgccgcCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGc < 1:593337/134‑1 (MQ=255) cATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTa > 1:120001/1‑134 (MQ=255) cGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTcccc < 1:1144363/134‑1 (MQ=255) aaGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAg > 1:1111471/1‑133 (MQ=255) ccAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACAGC‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGc > 1:387474/1‑133 (MQ=255) gATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATggcg > 1:918482/1‑42 (MQ=37) gATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATggcg < 2:918482/42‑1 (MQ=37) ccAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAAt < 1:579725/134‑1 (MQ=255) acac‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATgcgcgc > 1:31300/1‑134 (MQ=255) cac‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGcc < 2:120001/134‑1 (MQ=255) | GGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCC > AM260479/3302795‑3303046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |