New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260480	772858 =	28 (0.920)	6 (0.210)	6/198	1.8	17.7%	coding (1260/1389 nt)	rtxD2	RTX toxin membrane fusion protein
	?	AM260480	772887 =	29 (1.000)					coding (1289/1389 nt)	rtxD2	RTX toxin membrane fusion protein
Rejected: Frequency below/above cutoff threshold.

GCCGATACGCTGACACCGGAGCGCCCGGGCGAGCGCCCGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAACGATAAGAGCGGGCGGTTTGCCCACGACGACAAC  >  AM260480/772766‑773015                                                                                                                          |                                                                                                                                 gcCGATACGCTGACACCGGAGCGCCCGGGCGAGCGCCCGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGt                                                                                                                      >  2:817723/1‑134 (MQ=255)                       gcCCGGGCGAGCGCCAGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGa                                                                                                           >  2:863398/1‑123 (MQ=255)                       gcCCGGGCGAGCGCCAGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGa                                                                                                           <  1:863398/123‑1 (MQ=255)                         ccGGGCGAGCGCCCGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGAc                                                                                              >  1:315623/1‑134 (MQ=255)                             atgggcttgagcaggtaatggagcaccgtcttctgcccggtcagcacatcgaccgtcgccaccatgccgggcaggatgggcacctgcacggcatggtgg                                                                                                                             >  2:863840‑M2/94‑99 (MQ=255)                             atgggcttgagcaggtaatggagcaccgtcttctgcccggtcagcacatcgaccgtcgccaccatgccgggcaggatgggcacctgcacggcatggtgg                                                                                                                             <  1:863840‑M2/6‑1 (MQ=255)                                  cgcCCGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCa                                                                                     <  1:905792/134‑1 (MQ=255)                                        caggtaatggagcaccgtcttctgcccggtcagcacatcgaccgtcgccaccaTGCCGTGCAGGTGCCCATCCTGCCCGGCAtggtgg                                                                                                                             <  2:967338‑M1/35‑1 (MQ=255)                                        caggtaatggagcaccgtcttctgcccggtcagcacatcgaccgtcgccaccaTGCCGTGCAGGTGCCCATCCTGCCCGGCAtggtgg                                                                                                                             >  1:967338‑M1/54‑88 (MQ=255)                                                   ccTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCca                                                                    <  2:150345/134‑1 (MQ=255)                                                    cTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCcat                                                                   <  2:1104054/134‑1 (MQ=255)                                                    cTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCACATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCcat                                                                   <  2:363120/134‑1 (MQ=255)                                                     tGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCa                                                                                     <  2:306187/115‑1 (MQ=255)                                                     tGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCa                                                                                     >  1:306187/1‑115 (MQ=255)                                                                cgcACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAg                                                       >  1:62949/1‑134 (MQ=255)                                                                cgcACGCGCGACAACCGGCCAGGCGGCAGTCCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCAtcatca                                                              >  1:941535/1‑127 (MQ=255)                                                                cgcACGCGCGACAACCGGCCAGGCGGCAGTCCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCAtcatca                                                              <  2:941535/127‑1 (MQ=255)                                                                      cgcgACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCag                                                                                                   >  1:634760/1‑84 (MQ=255)                                                                      cgcgACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCag                                                                                                   <  2:634760/84‑1 (MQ=255)                                                                             aCCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTc                                          <  2:442240/134‑1 (MQ=255)                                                                                    aGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAAc                                   <  1:817723/134‑1 (MQ=255)                                                                                       ccaccatgccgggcaggatgggcacctgcacggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTg                                                                              >  1:967338‑M2/36‑88 (MQ=255)                                                                                       ccaccatgccgggcaggatgggcacctgcacggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTg                                                                              <  2:967338‑M2/53‑1 (MQ=255)                                                                                       ccaccaTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCcat                                                                   <  1:863840‑M1/93‑1 (MQ=255)                                                                                       ccaccaTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCcat                                                                   >  2:863840‑M1/7‑99 (MQ=255)                                                                                         gcAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCaa                                                                         <  1:725174/91‑1 (MQ=255)                                                                                         gcAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCaa                                                                         >  2:725174/1‑91 (MQ=255)                                                                                          cAGTGCCGTGCAGGTGCCCATCCTGCCCGGCGTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAACGATAag                             <  2:1018292/134‑1 (MQ=255)                                                                                                  ggcaggatgggcacctgcacggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCagaaga                                                                                               >  2:1110332‑M2/25‑60 (MQ=255)                                                                                                  ggcaggatgggcacctgcacggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCagaaga                                                                                               <  1:1110332‑M2/36‑1 (MQ=255)                                                                                                      ggTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTg                                                                                          <  1:918964/61‑1 (MQ=255)                                                                                                      ggTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTg                                                                                          >  2:918964/1‑61 (MQ=255)                                                                                                        tGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAACGATAAGAGCGGGCGGTTTGc               >  2:173860/1‑134 (MQ=255)                                                                                                           ccATCCTGCCCGGCATGGTGGCGACGGTCGTTGTGCTGACCGGGCagaaga                                                                                               <  1:941558/51‑1 (MQ=255)                                                                                                           ccATCCTGCCCGGCATGGTGGCGACGGTCGTTGTGCTGACCGGGCagaaga                                                                                               >  2:941558/1‑51 (MQ=255)                                                                                                              tCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAg                                                            >  2:200719/1‑83 (MQ=255)                                                                                                              tCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAg                                                            <  1:200719/83‑1 (MQ=255)                                                                                                                  gCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACg                                                                                             <  2:855518/46‑1 (MQ=255)                                                                                                                  gCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACg                                                                                             >  1:855518/1‑46 (MQ=255)                                                                                                                     cggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAACGATAAGAGCGGGCGGTTTGCCCACGACGacaac  <  1:173860‑M2/129‑1 (MQ=255)                                                                                                                     cggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAACGATAAGAGCGGGCGGTTTGCCCACGACGacaa   >  1:683286‑M2/6‑133 (MQ=255)                                                                                                                      ggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAAc                                   >  2:1160313‑M2/5‑100 (MQ=255)                                                                                                                      ggcaTGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAAc                                   <  1:1160313‑M2/96‑1 (MQ=255)                                                                                                                          |                                                                                                                                 GCCGATACGCTGACACCGGAGCGCCCGGGCGAGCGCCCGGAAAGCTACTACCTGGTGCGCGTGCGCACGCGCGACAACCGGCCAGGCGGCAGTGCCGTGCAGGTGCCCATCCTGCCCGGCATGGTGGCGACGGTCGATGTGCTGACCGGGCAGAAGACGGTGCTCCATTACCTGCTCAAGCCCATCATCAAGACCAGGGAACTGGCGTTCCGGGAACGATAAGAGCGGGCGGTTTGCCCACGACGACAAC  >  AM260480/772766‑773015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.