Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	342,407	T→C	L180L (TTG→CTG)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	342,407	0	T	C	100.0%	122.7 / NA	32	L180L (TTG→CTG)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

TGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAATTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCT  >  AM260479/342283‑342530                                                                                                                             |                                                                                                                            tGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                        <  1:813960/130‑1 (MQ=255)   gCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACtt                                                                                                                   <  1:1175100/133‑1 (MQ=255)           tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                            >  1:394790/1‑132 (MQ=255)                cTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                        >  2:274660/1‑115 (MQ=255)                cTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                        <  1:274660/115‑1 (MQ=255)                 ttCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTgcgc                                                                                                    >  2:559369/1‑134 (MQ=255)                                               accgaccgGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGatcat                                                                      >  1:1003158/1‑134 (MQ=255)                                                  gaccgGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCgg                                                                   >  1:66481/1‑134 (MQ=255)                                                       gCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATc                                                                                       <  2:224888/109‑1 (MQ=255)                                                       gCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATc                                                                                       >  1:224888/1‑109 (MQ=255)                                                                 aaGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                    <  2:762703/134‑1 (MQ=255)                                                                 aaGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                    <  1:664839/134‑1 (MQ=255)                                                                 aaGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                    <  1:276300/134‑1 (MQ=255)                                                                         gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGt                                            >  2:1008036/1‑134 (MQ=255)                                                                         gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGt                                            >  2:205038/1‑134 (MQ=255)                                                                         gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGt                                            >  2:541691/1‑134 (MQ=255)                                                                           cGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTtc                                          <  1:559369/134‑1 (MQ=255)                                                                           cGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTtc                                          <  2:593151/134‑1 (MQ=255)                                                                                    ccGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTg                                 >  2:1024912/1‑134 (MQ=255)                                                                                           cgccACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGgtgt                          >  2:559356/1‑134 (MQ=255)                                                                                                      gCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCtg               <  2:394790/134‑1 (MQ=255)                                                                                                       cAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCtgt              <  1:541691/134‑1 (MQ=255)                                                                                                       cAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCtgt              <  2:1003158/134‑1 (MQ=255)                                                                                                             cGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCa        <  1:205038/134‑1 (MQ=255)                                                                                                             cGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCa        <  1:1008036/134‑1 (MQ=255)                                                                                                               tggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCa        <  2:1149557/132‑1 (MQ=255)                                                                                                               tggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCa        >  1:1149557/1‑132 (MQ=255)                                                                                                               tggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCAcc      >  2:967939/1‑134 (MQ=255)                                                                                                                 gtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCtt    <  1:559356/134‑1 (MQ=255)                                                                                                                   ggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTtct  >  1:829520/1‑134 (MQ=255)                                                                                                                         ggAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                            >  2:7273/1‑38 (MQ=255)                                                                                                                         ggAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                            <  1:7273/38‑1 (MQ=255)                                                                                                                             |                                                                                                                            TGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAATTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCT  >  AM260479/342283‑342530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑