Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	406,488	G→T	L70I (CTC→ATC)	h16_A0388 ←	ABC‑type transporter, ATPase component: HAAT family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	406,488	0	G	T	100.0%	93.7 / NA	26	L70I (CTC→ATC)	h16_A0388	ABC‑type transporter, ATPase component: HAAT family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/13);  total (13/13)

TGCGGAATACCGAGGCTTCCTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGAGCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCAT  >  AM260479/406359‑406595                                                                                                                                  |                                                                                                            tGCGGAATACCGAGGCTTCCTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATg                                                                                                         >  1:697243/1‑134 (MQ=255)            gAGGCTTCCTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAggt                                                                                              >  2:702828/1‑134 (MQ=255)                      gCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGccc                                                                                    >  1:672314/1‑134 (MQ=255)                         gCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                 <  2:672314/134‑1 (MQ=255)                                          aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                 >  2:1164783/1‑117 (MQ=255)                                          aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                 <  1:1164783/117‑1 (MQ=255)                                          aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAg                                                                >  2:825240/1‑134 (MQ=255)                                           tGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAgg                                                               >  1:475882/1‑134 (MQ=255)                                               cgcgcgcTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGa                                                           >  1:48613/1‑134 (MQ=255)                                                    gcTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTg                                                                                       >  1:474666/1‑101 (MQ=255)                                                    gcTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTg                                                                                       <  2:474666/101‑1 (MQ=255)                                                                gCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTc                                          <  1:702828/134‑1 (MQ=255)                                                                    gCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGt                                      >  2:762637/1‑134 (MQ=255)                                                                               ggTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACg                           >  2:885974/1‑134 (MQ=255)                                                                                     cGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCtt                      >  2:52537/1‑133 (MQ=255)                                                                                      gTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGa                    <  1:825240/134‑1 (MQ=255)                                                                                            cACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCg              <  2:697243/134‑1 (MQ=255)                                                                                            cACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCg              <  2:475882/134‑1 (MQ=255)                                                                                            cACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCg              <  1:266423/134‑1 (MQ=255)                                                                                                     gCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAg      <  2:48613/133‑1 (MQ=255)                                                                                                       cTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCa   <  1:885974/134‑1 (MQ=255)                                                                                                        ttCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGc     >  2:213045/1‑131 (MQ=255)                                                                                                        ttCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGc     <  1:213045/131‑1 (MQ=255)                                                                                                          cGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCAt  <  2:513496/132‑1 (MQ=255)                                                                                                               aGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                 >  2:241790/1‑48 (MQ=255)                                                                                                               aGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                 <  1:241790/48‑1 (MQ=255)                                                                                                                                  |                                                                                                            TGCGGAATACCGAGGCTTCCTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGAGCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCAT  >  AM260479/406359‑406595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑