Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 293,407 T→A Y759N (TAC→AAC)  flhF → Flagellar GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480293,4070TA100.0% 144.8 / NA 38Y759N (TAC→AAC) flhFFlagellar GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/18);  total (20/18)

CGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCC  >  AM260480/293291‑293526
                                                                                                                    |                                                                                                                       
cgcccgccTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGc                                                                                                        <  2:817790/134‑1 (MQ=255)
                                    tGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAg                                                                    >  1:1033479/1‑134 (MQ=255)
                                          aGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                              <  1:369732/134‑1 (MQ=255)
                                                  ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                   <  2:375595/105‑1 (MQ=255)
                                                  ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                   >  1:375595/1‑105 (MQ=255)
                                                  ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGcc                                                      <  2:124564/134‑1 (MQ=255)
                                                          gggCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGAc                                                >  1:1055432/1‑132 (MQ=255)
                                                          gggCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGAc                                                >  2:261244/1‑132 (MQ=255)
                                                          gggCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGAc                                                >  2:442726/1‑132 (MQ=255)
                                                               cGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGcc                                          >  1:318374/1‑133 (MQ=255)
                                                                      cAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                   <  2:840047/85‑1 (MQ=255)
                                                                      cAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                   >  1:840047/1‑85 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                 <  2:1100229/77‑1 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                 >  1:1100229/1‑77 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                              <  1:140586/96‑1 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                              >  2:140586/1‑96 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGcc                                                            >  1:414432/1‑98 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGcc                                                            <  2:414432/98‑1 (MQ=255)
                                                                                aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                        >  2:317963/1‑134 (MQ=255)
                                                                                  gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCa                                                                     <  1:583099/87‑1 (MQ=255)
                                                                                  gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCa                                                                     >  2:583099/1‑87 (MQ=255)
                                                                                  gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                               <  2:912960/93‑1 (MQ=255)
                                                                                  gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                               >  2:943198/1‑93 (MQ=255)
                                                                                  gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                               <  1:943198/93‑1 (MQ=255)
                                                                                  gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                               >  1:912960/1‑93 (MQ=255)
                                                                                    gCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTgg                    <  1:442726/134‑1 (MQ=255)
                                                                                     ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGgtg                                                                          >  1:823928/1‑79 (MQ=255)
                                                                                     ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGgtg                                                                          <  2:823928/79‑1 (MQ=255)
                                                                                     ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGagag                                     <  1:876425/116‑1 (MQ=255)
                                                                                     ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGagag                                     >  2:876425/1‑116 (MQ=255)
                                                                                     ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGgc                   >  2:123590/1‑134 (MQ=255)
                                                                                       gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGgcgc                 <  2:318374/134‑1 (MQ=255)
                                                                                       gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGgcgc                 >  1:879367/1‑134 (MQ=255)
                                                                                                        gACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGcc  <  2:879367/132‑1 (MQ=255)
                                                                                                         aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacga        <  2:224065/125‑1 (MQ=255)
                                                                                                         aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacga        >  1:224065/1‑125 (MQ=255)
                                                                                                           gTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                              <  2:307908/69‑1 (MQ=255)
                                                                                                           gTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                              >  1:307908/1‑69 (MQ=255)
                                                                                                                    |                                                                                                                       
CGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCC  >  AM260480/293291‑293526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑