Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 433,189 A→C R133R (CGA→CGC)  h16_B0381 → Acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480433,1890AC100.0% 146.4 / NA 38R133R (CGA→CGC) h16_B0381Acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/21);  total (17/21)

ATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGATACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCC  >  AM260480/433061‑433316
                                                                                                                                |                                                                                                                               
aTGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACgg                                                                                                                            >  1:1021263/1‑134 (MQ=255)
         tCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCt                                                                                                                   >  1:900667/1‑134 (MQ=255)
               aTGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCa                                                                                                             >  2:458226/1‑134 (MQ=255)
                tGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCAc                                                                                                            <  2:900667/134‑1 (MQ=255)
                  aaGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACg                                                                                                           <  1:957352/133‑1 (MQ=255)
                         tGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                   <  1:31718/134‑1 (MQ=255)
                           aTGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                   <  1:1113865/132‑1 (MQ=255)
                           aTGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                   >  2:1113865/1‑132 (MQ=255)
                             gCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                             <  2:504826/104‑1 (MQ=255)
                             gCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                             >  1:504826/1‑104 (MQ=255)
                                        aCCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTAc                                                                                    <  2:905050/134‑1 (MQ=255)
                                          ctgctgCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTg                                                                                  <  2:1021263/134‑1 (MQ=255)
                                          ctgctgCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTg                                                                                  <  1:850530/134‑1 (MQ=255)
                                                 ttGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGa                                                                                              <  2:259725/115‑1 (MQ=255)
                                                 ttGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGa                                                                                              >  1:259725/1‑115 (MQ=255)
                                                      ggCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                     >  1:1031209/1‑103 (MQ=255)
                                                      ggCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                     <  2:1031209/103‑1 (MQ=255)
                                                       gCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                     >  2:276530/1‑102 (MQ=255)
                                                       gCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                     <  1:276530/102‑1 (MQ=255)
                                                       gCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTggag                                                                     >  2:406548/1‑134 (MQ=255)
                                                              cAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGcc                                                               <  1:804103/133‑1 (MQ=255)
                                                                       ggtggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                             <  1:1005439/62‑1 (MQ=255)
                                                                       ggtggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                             >  2:1005439/1‑62 (MQ=255)
                                                                        gtggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCa                                                    <  1:406548/134‑1 (MQ=255)
                                                                         tggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCAc                                                   >  1:845935/1‑134 (MQ=255)
                                                                         tggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCAc                                                   <  2:807682/134‑1 (MQ=255)
                                                                            caggcggcATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                 >  1:498856/3‑133 (MQ=255)
                                                                                cggcATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATgg                                            <  1:458226/134‑1 (MQ=255)
                                                                                                 ccAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                             <  2:706602/36‑1 (MQ=37)
                                                                                                 ccAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                             >  1:706602/1‑36 (MQ=37)
                                                                                                                        cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                 <  2:130270/41‑1 (MQ=255)
                                                                                                                        cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                 >  1:130270/1‑41 (MQ=255)
                                                                                                                        cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATggg    <  1:513047/134‑1 (MQ=255)
                                                                                                                          cgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGcc  >  2:1001973/1‑134 (MQ=255)
                                                                                                                            gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCAc                                                   <  1:308714/83‑1 (MQ=255)
                                                                                                                            gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCAc                                                   >  2:308714/1‑83 (MQ=255)
                                                                                                                            gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGa                                               >  1:742640/1‑87 (MQ=255)
                                                                                                                            gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGa                                               <  2:742640/87‑1 (MQ=255)
                                                                                                                                |                                                                                                                               
ATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGATACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCC  >  AM260480/433061‑433316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑