BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	753,133	T→C	K37E (AAG→GAG)	h16_B0675 ←	Acyl‑CoA dehydrogenase,short‑chain specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	753,133	0	T	C	100.0%	148.4 / NA	38	K37E (AAG→GAG)	h16_B0675	Acyl‑CoA dehydrogenase,short‑chain specific
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/19); total (19/19)

CGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAAC  >  AM260480/753013‑753262

cgcgCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcgcgcgcg                                                                                                                      >  2:642880/1‑134 (MQ=255)

       caccacCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTg                                                                                                                              <  1:683063/119‑1 (MQ=255)

       caccacCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTg                                                                                                                              >  2:683063/1‑119 (MQ=255)

       caccacCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCtt                                                                                                                 <  1:642880/132‑1 (MQ=255)

                ctggctggCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCt                                                                                                      <  1:297260/134‑1 (MQ=255)

                   gctggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCg                                                                                                    >  1:391573/1‑133 (MQ=255)

                   gctggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCg                                                                                                    >  2:119376/1‑133 (MQ=255)

                     tggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGc                                                                                                        >  1:160004/1‑127 (MQ=255)

                     tggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGc                                                                                                        <  2:160004/127‑1 (MQ=255)

                        cGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg                                                                                                                            <  2:680121/104‑1 (MQ=255)

                        cGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg                                                                                                                            >  1:680121/1‑104 (MQ=255)

                                     gAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCCCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGa                                                                                 <  2:299093/134‑1 (MQ=255)

                                                                           cGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGtt                                                <  2:604610/129‑1 (MQ=255)

                                                                           cGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGtt                                                >  1:604610/1‑129 (MQ=255)

                                                                             cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGc                                                                                                        >  1:307173/1‑71 (MQ=255)

                                                                             cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGc                                                                                                        <  2:307173/71‑1 (MQ=255)

                                                                             cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTc                                         >  2:318504/1‑134 (MQ=255)

                                                                                 tttCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTg                                     >  2:604891/1‑134 (MQ=255)

                                                                                           cTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCa                                                      <  1:826572/107‑1 (MQ=255)

                                                                                           cTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCa                                                      >  2:826572/1‑107 (MQ=255)

                                                                                              gTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTc                          <  2:391573/132‑1 (MQ=255)

                                                                                                tCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATg                      >  2:1043534/1‑134 (MQ=255)

                                                                                                  cacaCGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGt                     >  1:388989/1‑133 (MQ=255)

                                                                                                  cacaCGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGt                     <  1:312818/133‑1 (MQ=255)

                                                                                                  cacaCGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTc                    <  1:604891/134‑1 (MQ=255)

                                                                                                   acacGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCg                                        >  1:659401/1‑113 (MQ=255)

                                                                                                   acacGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCg                                        <  2:659401/113‑1 (MQ=255)

                                                                                                   acacGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGt                     >  2:30834/1‑132 (MQ=255)

                                                                                                        ccgcGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTAt              >  1:810226/1‑134 (MQ=255)

                                                                                                         cgcgATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGATGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCg                                        <  1:310272/107‑1 (MQ=255)

                                                                                                         cgcgATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGATGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCg                                        >  2:310272/1‑107 (MQ=255)

                                                                                                            gATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCt          <  1:119376/134‑1 (MQ=255)

                                                                                                             aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGt                                <  1:703778/111‑1 (MQ=255)

                                                                                                             aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGt                                >  2:703778/1‑111 (MQ=255)

                                                                                                             aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCtt         <  2:810226/134‑1 (MQ=255)

                                                                                                             aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCtt         >  1:1058177/1‑134 (MQ=255)

                                                                                                                cAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAg      <  1:318504/134‑1 (MQ=255)

                                                                                                                    ctctCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAAc  <  1:30834/134‑1 (MQ=255)

CGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAAC  >  AM260480/753013‑753262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`