Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	1,540,787	T→C	K465R (AAG→AGG)	coxL6 ←	Aerobic‑type carbon monoxide dehydrogenase homolog, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	1,540,787	0	T	C	100.0%	116.9 / NA	31	K465R (AAG→AGG)	coxL6	Aerobic‑type carbon monoxide dehydrogenase homolog, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

GAGCGCGATCGGGAACGGATAAGGACTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCTTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCCGCGCACGTCGTCTTCGCGCGTC  >  AM260480/1540660‑1540885                                                                                                                                |                                                                                                   gAGCGCGATCGGGAACGGATAAGGACTGCCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAg                                                                                               <  2:896670/133‑1 (MQ=255)  aGCGCGATCGGGAACGGATAAGGACTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGc                                                                                             >  2:1059712/1‑134 (MQ=255)    cgcgATCGGGAACGGATAAGGACTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGc                                                                                             >  1:432359/1‑132 (MQ=255)            ggAACGGATAAGGACTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCAccttg                                                                                   >  1:954406/1‑134 (MQ=255)            ggAACGGATAAGGACTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCAccttg                                                                                   >  2:742971/1‑134 (MQ=255)                         aCTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGt                                                                      <  1:919401/134‑1 (MQ=255)                          cTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTg                                                                     >  1:819785/1‑134 (MQ=255)                               gCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGa                                                                >  2:775601/1‑134 (MQ=255)                                 tCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATg                                                              >  2:48975/1‑134 (MQ=255)                                   aCGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTc                                                            <  1:775601/134‑1 (MQ=255)                                           gACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCggt                                                    >  2:559267/1‑134 (MQ=255)                                                   tcgagtcgaCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAg                                                                                          <  1:706059/88‑1 (MQ=255)                                                   tcgagtcgaCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAg                                                                                          >  2:706059/1‑88 (MQ=255)                                                     gagtcgaCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCAt                                          >  2:608812/1‑134 (MQ=255)                                                     gagtcgaCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCAt                                          <  1:608812/134‑1 (MQ=255)                                                     gagtcgaCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCAt                                          <  2:954406/134‑1 (MQ=255)                                                       gtcgaCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCg                                        <  1:1059712/134‑1 (MQ=255)                                                           aCCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCg                                    >  1:1146302/1‑134 (MQ=255)                                                                          gTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGt                                                                      <  1:822894/85‑1 (MQ=255)                                                                          gTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGt                                                                      >  2:822894/1‑85 (MQ=255)                                                                          gTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCcgcg                     <  1:559267/134‑1 (MQ=255)                                                                                     gAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCCGCGCACgtcgtc            <  2:1146302/132‑1 (MQ=255)                                                                                       aCGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCcgcg                     <  1:897060/121‑1 (MQ=255)                                                                                       aCGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCcgcg                     >  2:897060/1‑121 (MQ=255)                                                                                       aCGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCCGCGCACGTCGTCTTcg        <  1:742971/134‑1 (MQ=255)                                                                                         ggCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCa                                                                                                >  1:852092/1‑44 (MQ=255)                                                                                         ggCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCa                                                                                                <  2:852092/44‑1 (MQ=255)                                                                                         ggCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCCGCGCACGTCGTCTTcgcg      >  2:832927/1‑134 (MQ=255)                                                                                                 gCTGGTGATGGACTGGCCGACGATCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCCGCGCACGTCGTCTTCGCGCGTc  >  1:996992/1‑130 (MQ=255)                                                                                                                    acgaTCACGTGCCTCCAGGCCAGCCCCTTGCCCTTGCCATCGTGGCCg                                                                 <  1:819742/48‑1 (MQ=38)                                                                                                                    acgaTCACGTGCCTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCg                                                                 >  2:819742/1‑48 (MQ=255)                                                                                                                                |                                                                                                   GAGCGCGATCGGGAACGGATAAGGACTGTCGCTCACGCCCTCGACCATGGTCGAGTCGACCCCGTTCTTCACGGTGAAGGACTCGAACGGCGTGCCGCTGGTGATGGACTGGCCGACGATCACGTGCTTCCAGGCCAGCACCTTGCCCTTGCCATCGTGGCCGATGTCAACGCGGTGCAGGTGCATCGGGCGGTAGTAGCCGCCGCGCACGTCGTCTTCGCGCGTC  >  AM260480/1540660‑1540885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑