Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,256,779	G→A	P94S (CCG→TCG)	h16_B1998 ←	transcriptional regulator, XRE‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,256,779	0	G	A	100.0%	147.7 / NA	39	P94S (CCG→TCG)	h16_B1998	transcriptional regulator, XRE‑family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/19);  total (20/19)

TGGCCATTGGCTGGTCTTGTGCCAGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGGAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTACCGATGGTCGGCGACGACAGGCCG  >  AM260480/2256650‑2256909                                                                                                                                  |                                                                                                                                   tggcCATTGGCTGGTCTTGTGCCAGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAAcc                                                                                                                                >  2:1051098/1‑134 (MQ=255) tggcCATTGGCTGGTCTTGTGCCAGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAAcc                                                                                                                                >  2:819098/1‑134 (MQ=255)                   gtgCCAGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGa                                                                                                                <  1:819098/132‑1 (MQ=255)                       cAGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACcagca                                                                                                          <  1:1054276/134‑1 (MQ=255)                        aGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGcc                                                                                                                    >  1:156324/1‑123 (MQ=255)                        aGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGcc                                                                                                                    <  2:156324/123‑1 (MQ=255)                                                 aGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTg                                                                                >  1:35843/1‑134 (MQ=255)                                                     cTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCt                                                                                                                   <  2:782170/95‑1 (MQ=255)                                                     cTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCt                                                                                                                   >  1:782170/1‑95 (MQ=255)                                                     cTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGa                                                                            >  2:676291/1‑134 (MQ=255)                                                      tGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGAt                                                                           >  1:949386/1‑134 (MQ=255)                                                       gCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGa                                                                                                                <  2:1148687/96‑1 (MQ=255)                                                       gCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGa                                                                                                                >  1:1148687/1‑96 (MQ=255)                                                             ccTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTgg                                                                    <  2:35843/134‑1 (MQ=255)                                                             ccTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTgg                                                                    <  1:676291/134‑1 (MQ=255)                                                                           tCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGc                                                                                      <  1:146067/102‑1 (MQ=255)                                                                           tCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGc                                                                                      >  2:146067/1‑102 (MQ=255)                                                                                ggCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGAt                                                                           >  2:693454/1‑108 (MQ=255)                                                                                ggCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGAt                                                                           <  1:693454/108‑1 (MQ=255)                                                                                  cAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGcc                                               <  2:949386/134‑1 (MQ=255)                                                                                    aTGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTc                                             <  2:735266/134‑1 (MQ=255)                                                                                         ttCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGc                                          >  2:304388/1‑132 (MQ=255)                                                                                               ggTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTg                                   <  1:1051098/133‑1 (MQ=255)                                                                                                      cccTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGcc                                               >  2:552447/1‑114 (MQ=255)                                                                                                      cccTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGcc                                               <  1:552447/114‑1 (MQ=255)                                                                                                       ccTGGCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTa                          >  1:963563/1‑134 (MQ=255)                                                                                                          ggCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTAcc                        >  2:1104579/1‑133 (MQ=255)                                                                                                          ggCGCTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTAcc                        >  1:488356/1‑133 (MQ=255)                                                                                                           gcgcTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTACCg                       <  1:1104579/133‑1 (MQ=255)                                                                                                              cTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGc                                                                                      <  1:656378/67‑1 (MQ=255)                                                                                                              cTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGc                                                                                      >  2:656378/1‑67 (MQ=255)                                                                                                              cTCGGCGGACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTACCGATgg                   >  1:193948/1‑134 (MQ=255)                                                                                                                     gACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCt                                     <  2:1152695/109‑1 (MQ=255)                                                                                                                     gACGGGCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCt                                     >  1:1152695/1‑109 (MQ=255)                                                                                                                         ggCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAgg                                                                                                        >  2:404041/1‑38 (MQ=255)                                                                                                                         ggCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAgg                                                                                                        <  1:404041/38‑1 (MQ=255)                                                                                                                         ggCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAgg                                                                                                        >  2:810542/1‑38 (MQ=255)                                                                                                                         ggCTGAACGAAACCCGGTCCGCGGCCTTGACCAGCAgg                                                                                                        <  1:810542/38‑1 (MQ=255)                                                                                                                               aCGAAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTACCGATGGTCGGCGACGACAGGCcg  <  2:193948/134‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   TGGCCATTGGCTGGTCTTGTGCCAGCGTGAACGGCGGCACCATGACGAAGGGCTGCATCGCCTTGTCCACGCGCTCCGTGGCAATGGCTTCGTAGGTATAGCCCTGGCGCTCGGCGGACGGGCTGAACGGAACCCGGTCCGCGGCCTTGACCAGCAGGATCTCATTGCCGCGCTGCGGCTTGGCGATCAGCTGGTCGACGGTCACGTCGAGGGCCTCGGCCAGCTTGAGCACCGTACCGATGGTCGGCGACGACAGGCCG  >  AM260480/2256650‑2256909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑